medigraphic.com
ISSN 1027-2852 (Electronic)
ISSN 0864-4551 (Print)

2022, Number 4

<< Back

Biotecnol Apl 2022; 39 (4)

In vitro regeneration of Mortiño plants (Vaccinium floribundum Kunth) by induced callogenesis

Meneses MLS, Egas CFX, Vásquez CW, Morillo VLE

Language: English
References: 29
Page: 4211-4215
PDF size: 565.94 Kb.


ABSTRACT

Mortiño (Vaccinium floribundum Kunth) is an Andean fruit tree, increasingly popular due to its flavor, nutritional value, and it is regarded as a functional food. However, there are no effective methodologies for its propagation, and tissue culture technologies for its availability need to be developed despite prior studies on the micropropagation of Mortiño seedlings. Therefore, in this work, callogenesis was evaluated as an alternative for in vitro regeneration of Mortiño plants. Callus formation was induced starting from foliar tissue of Mortiño vitroplants. Explants were grown in Murashige & Skoog-MS ½ and Woody Plant Medium (WPM) culture media, supplemented with cytokinins Thidiazuron (TDZ, 1.5 and 3.0 mg/L), Zeatin (ZEA) and Trans-zeatin (TZR), both at 2.0 and 4.0 mg/L. Plant material was incubated at either 16 h light/8 h dark or full darkness. A favorable response was observed in WPM supplemented with 1.5 mg/L TDZ, with 65.0 and 81.7 % of adventitious shoots developed at 16h light/8 h dark and full darkness, respectively. These results show promising for the regeneration of Mortiño plants by indirect organogenesis.


REFERENCES

  1. Sanjinés A, Ollgaard B, Balslev H. Frutos comestibles. In: Moraes M, Ollgaard RB, Kvist LP, Borchsenius F, Balslev H, editores. Botánica Económica de los Andes Centrales. La Paz: Universidad Mayor de San Andes; 2006. p.329-46.

  2. Coba P, Coronel D, Verdugo K, Paredes M, Yugsi E, Huachi L. Estudio etnobotánico del mortiño (Vaccinium floribundum) como alimento ancestral y potencial alimento funcional. La Granja. 2012;16:5-13.

  3. Racines-Oliva MA, Hidalgo-Verdezoto MR, Vasquez-Castillo WA. Domesticación de mortiño (Vaccinium floribundum Kunth.): frutal andino con gran potencial para la industria alimenticia. Agron Colombiana. 2016;34:S51-3.

  4. Aguirre Nathalia. Evaluación in vitro de la calidad de la semilla botánica de mortiño (Vaccinium floribundum) de los Andes ecuatorianos. Tesis de pregrado Ingeniería Agroindustrial. Quito: Universidad de las Americas; 2020.

  5. Vasco C, Riihinen K, Ruales J, KamalA. Chemical composition and phenoliccompound profile of mortiño (Vacciniumfloribundum Kunth). J Agric Food Chem.2009;57:8274-81.

  6. Aguirre P. Alimentos funcionales entrelas nuevas y viejas corporalidades. RevAntropol Iberoameric. 2017;14:95-120.

  7. Fuentes-Berrio L, Acevedo-Correa D,Gelvez Ordoñez V M. Alimentos Funcionales:impacto y retos para el desarrolloy bienestar de la sociedad colombiana.Biotecnología en el sector Agropecuario yAgroindustrial. 2015;13:140-9.

  8. Meléndez-Jácome MR, Flor-Romero LE,Sandoval-Pacheco ME, Vasquez-CastilloWA, Racines-Oliva M A. Vaccinium spp.:Karyotypic and phylogenetic characteristics,nutritional composition, edaphoclimaticconditions, biotic factors and beneficialmicroorganisms in the rhizosphere. ScientiaAgrop. 2021;12:109-20.

  9. Cobo M, Gutierrez B, Torres M. Regenerationon mortiño (Vaccinium floribundumKunth) plants through axillary bud culture.In vitro Cell Dev Biol Plant. 2018;54:112-6.

  10. Meneses S, Morillo E, Vásquez W. Invitro propagation of Vaccinium floribundumKunth from seeds: promissory technology formortiño accelerated production. Can J PlantSci. 2022;102(1):216-224.

  11. Chaparro M, Becerra N. Anatomía delfruto de Vaccinium floribundum (Ericaceae).Acta Biol Colombiana. 1999;4:47-60.

  12. Martín R, Chong B, Pérez N. Organogénesisin vitro en el género Digitalis. BiotecnolVeg. 2015;15:195-206.

  13. Murashige T, Skoog F. A revised mediumfor the rapid growth and bioassaywith tobacco tissue cultures. Physiol Plant.1962;15:473-97.

  14. McCoen BH, Lloyd G. Woody plant medium(WPM) – A mineral nutrient formulationfor microculture of woody plant species.HortScience. 1981;16:453.

  15. Grupo InfoStat. InfosStat. Córdoba: FCA.Universidad Nacional de Córdoba; 2016.

  16. Ruzic D, Vujovic T, Libiakoba G, CerovicR, Gajdosova A. Micropropagation in vitro ofhighbush blueberry (Vaccinium corymbosumL.). J Berry Res. 2012;2:97-103.

  17. Martínez-Villegas YM, Andrade-RodríguezM, Colinas-León TM, Villegas-Torres OG,Castillo-Gutiérrez A, Alia-Tejacal I. Efecto delas sales inorgánicas del medio de cultivo enel crecimiento de Pascuita (Euphorbia leucocephalaLosty). Fitotec Mex. 2015;38:369-74.

  18. Rodríguez M, Chacón M, Carrillo R. Efectode la concentración de los componentes delmedio de cultivo MS sobre la germinación in vitrode Ugni molinae. Bosque. 2014;35:119-22.

  19. Azofeifa A. Problemas de oxidación yoscurecimiento de explantes cultivados in vitro.Agron Mesoameric. 2009;20(1):153-75.

  20. Brenes A, Castillo R, Gómez L. Micropropagaciónde cuatro cultivares de arándano (Vacciniumspp.) a partir de segmentos foliares de dos procedencias.Agron Costarricense. 2015;39:7-23.

  21. Cedres M, Sharry S, Adema M, AbediniW, Iannicelli J, Escandon A, et al. Plantas deprobeta: Manual para la propagación deplantas por cultivo de tejidos in vitro. La Plata:Universidad de La Plata; 2015.

  22. Das T, Mitra G. Micropropagación ofEucaliptus tereticornis Smith. Plant Cell TissueOrgan Cult. 1990;22:95-103.

  23. Marks T, Simpson E. Reduce phenolicoxidation at culture initiation in vitro followingthe exposure of field-grown stock plantsto darkness or low levels of irradiance. JHorticult Sci. 1990;65:103-11.

  24. Sánchez MC, Salaverría JL. Control dela oxidación en el cultivo in vitro de fresa(Fragaria X ananassa Duch.). Rev UDO Agrícola.2004;4:21-6.

  25. Hide A, Abdelnour A. Establecimiento invitro de arándano (Vaccinium corymbosumL). Tecnología en Marcha. 2013;26:64-71.

  26. Mok M, Mok D. The metabolism of (14C)-thidiazuron in callus tissues of Phaseoluslunatus [cotton defoliant]. Physiol Plant.1985;4:427-32.

  27. Murthy B, Murch S, Saxena P. Thidiazuron:A potent regulator of in vitro plantmorphogenesis. In vitro Cell Dev Biol Plant.1998;34:267-75.

  28. Debnath SC. Adventitious shoot regenerationin a bioreactor system and EST-PCRbased clonal fidelity in lowbush blueberry(Vaccinium angostifolium Ait). Scientia Horticult.2011;128:124-30.

  29. Scalzo J, Donno D, Miller S, Ghezzi M,Mellano MG, Cerutti AK, et al. Effect ofgenotype, medium and light on in vitro plantproliferation of Vaccinium spp. New Zealand JCrop Horticult Sci. 2016;44:231-46.




2020     |     www.medigraphic.com