medigraphic.com
ISSN 1027-2852 (Electronic)
ISSN 0864-4551 (Print)

2022, Number 2

Next >>

Biotecnol Apl 2022; 39 (2)

Physiological working ranges of hematological and serum biochemical parameters in BALB/C, NMRI and C57/BL6 mice strains for preclinical experimentation in Cuba

Aldana L, Puente P, Valdés I, Martínez R, Hernández A, Castro J

Language: English
References: 39
Page: 2201-2205
PDF size: 375.79 Kb.


ABSTRACT

The availability of standardized ranges of values for physiological parameters (hematological and serum biochemical parameters) is essential for establishing the health status of animals in experimentation. They are required also to evaluate the effects of different natural and biotechnological products once applied, and, remarkably, they are influenced by a series of factors (genetics, housing, feeding and handling, among others). Unfortunately, reference values for these parameters adapted to working conditions in Cuba were unavailable. Therefore, this study was aimed to establish the physiological working range of hematological and serum biochemical parameters of BALB/C, NMRI and C57/BL6 mice lines. For this purpose, 360 animals were tested for hematological and serum biochemical parameters, 120 samples (60 male and 60 female animals) were taken for each mouse strain (BALB/C, NMRI and C57/BL6), and results were compared between both sexes in each strain, and among animals of the same sex in different strains. Relevant differences were found between males and females in BALB/C mice in 40 % of the studied parameters, while 63 % in C57/BL6 and 22 % in NMRI mice strains and all the parameters were infl uenced in both sexes. Therefore, our results bring support to researchers in assessing the health status of these mice strains in preclinical studies. More importantly, these are functional working ranges of the physiological parameters analyzed for animal experimentation in toxicology determinations in Cuba. They must be carefully considered, since the establishment of reference values could involve a higher number of animals as set for other strains as the Charles River.


REFERENCES

  1. Bernard J, Sandford T. Diagnóstico y Tratamiento Clínicos por el Laboratorio. 7th ed. Barcelona: Salvat editors; 1984.

  2. Capecchi MR. Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century. Nat Rev Genet. 2005;6:507-12.

  3. Craigen WJ. Mouse models of humangenetic disorders. In: Scriver CR, BeaudetAL, Valle D, Sly WS, Childs B, Kinzier KW,et al. (eds). The Metabolic and MolecularBases of Inherited Disease. 8th. ed. NewYork: McGraw-Hill; 2001. p. 379-416.

  4. Emes RD, Goodstadt L, Winter EE, PontingChris P. Comparison of the genomesof human and mouse lays the foundationof genome zoology. Hum Mol Genet.2003;12:701-9.

  5. Justice MJ, Siracusa LD, Stewart AF.Technical approaches for mouse models ofhuman disease. Dis Models Mechanisms.2011;4:305-10.

  6. National Research Council (US) Committeeon Recognition and Alleviation ofDistress in Laboratory Animals. Recognitionand Alleviation of Distress in LaboratoryAnimals. Washington (DC): National AcademiesPress (US); 2008 [cited 2022 Jan 16].4, Avoiding, Minimizing, and AlleviatingDistress. Available from: https://www.ncbi.nlm.nih.gov/books/NBK4039/

  7. McLaughlin C, Prowse KR. Referenceblood chemistry values throughout thelifespan of M. spretus. Contemp Top LabAnim Sci. 1999;38:70-2.

  8. Boza C, Linares J, Quesada D, FernándezN, Carmenate L, Rivero D, et al.Characterization of the normative biologyof the CAnN.Cg-Foxn1nu/Cenp mouseline. Rev Fesahancccal. 2021;7(1):10-6.

  9. Esquivel N, García Y, Lores B, GutiérrezM, Rodríguez C. Characterization ofaged male BALB/ccenp mice as a modelof dementia. Lab Anim Res. 2020;36:7.

  10. National Research Council (US) Committeefor the Update of the Guide forthe Care and Use of Laboratory Animals.Guide for the Care and Use of LaboratoryAnimals. 8th edition. Washington (DC):National Academies Press (US); 2011 [cited2022 Jan 16]. Available from: https://www.ncbi.nlm.nih.gov/books/NBK54050/

  11. Center for the State Control ofDrugs, Equipment and Medical Devices.Resolution No. 11/2013. Regulation No.64/2013. Guidelines for the Constitutionand Functioning of the Institutional Committeesfor the Care and Use of LaboratoryAnimals (CICUAL). 2013 [cited 2021 Nov04]. Available from: https://www.cecmed.cu/reglamentacion/aprobadas/resolucioncecmed-11

  12. Roe FJ. Influence of animal species,strain, age, hormonal and nutritionalstatus. In: Anderson D, Conning DM(eds.). Experimental toxicology, the Basicissues. 2nd ed. Cambridge: Royal Societyof Chemistry; 1993, p. 23-34.

  13. Balkaya M, Voyvoda H, Ünsal C, ÇelerH. Some hematological and biochemicalcharacteristics of male and femaleSprague-Dawley rats. J Fac Vet Med UnivMed Univ. 2001;27(1):37-47.

  14. Barbosa BS, Praxedes EA, Lima MA,Lustosa Pimentel MM, Araujo Santos F,Dias Brito P, et al. Perfil hematológico ebioquímico de camundongos da linhagemBalb-c. Acta Sci Vet. 2017;45:1477.

  15. Archer RK, Festing MF, Riley JW. Hematologyof conventionally maintainedLac: Pouthbred Wistar rats during the 1styear of life. Lab Anim. 1982;16:198-200.

  16. Coles EH. Veterinary clinical pathology.4th. ed. Philadelphia: W.B. SaundersCompany; 1986.

  17. Schalm OV, Jain NC. Schalm’s veterinaryhematology. 4th ed. Philadelphia: Lea& Febiger; 1986.

  18. Mays A. Baseline hematological andblood biochemical parameters of theMongolian gerbil (Meriones unguiculatus).Lab Anim Care. 1969;19:838-42.

  19. Aleman CL, Noa R, Mas I, MenéndezR, Gámez R, Hernández C. Reference datafor the principal physiological indicators inthree species of laboratory animals. LabAnimals. 2000;34:379-85.

  20. Charles Rivers. Research Models [Internet].BALB/C Mouse Hematology. NorthAmerican Colonies January 2008 - December2012. c2022 [cited 2021 Dec 12].Available in: http://animalab.eu/sites/all/pliki/produkty-dopobrania/balb_c_Mouse_clinical_pathology_data.pdf

  21. Restel TI. Avaliação ponderal, hematólogicae bioquímica em camundongosMus musculus, linhagem Swiss, machos efêmeas de diferentes idades, do biotériocentral da Universidade Federal de MatoGrosso do Sul, Campo Grande, MS. 72f.Thesis (MSc) - Programa de Pós-graduaçãoem Saúde e Desenvolvimento na RegiãoCentro-Oeste, Universidade Federal deMato Grosso do Sul; 2012.

  22. Kile BT, Mason CL, Justice MJ. Sex andstrain-related differences in the peripheralblood cell values of inbred mouse strains.Mamm Gen. 2003;14:81-5.

  23. Wira CR, Rodriguez M, Patel MV. Therole of sex hormones in immune protectionof the female reproductive tract. Nat RevImmunol. 2015;15:217-30.

  24. Mazzaccara C, Labruna G, Cito G,Scarfò M, De Felice M, Pastore L, et al. Agerelatedreference intervals of the main biochemicaland hematological parameters inC57BL/6J, 129SV/EV and C3H/HeJ mousestrains. PLoS ONE. 2008;3(11):e3772.

  25. Doeing DC, Borowicz JL, CrockettET. Gender dimorphism in differentialperipheral blood leukocyte counts in miceusing cardiac, tail, foot, and saphenousvein puncture methods. BMC Clin Pathol.2003;12:3-9.

  26. Sakazaki H, Ueno H, Nakamuro K.Estrogen receptor alpha in mouse spleniclymphocytes: possible involvement in immunity.Toxicol Lett. 2002;133(2-3):221-9.

  27. Hoffman-Goetz L. Effect of estradioland exercise on lymphocyte proliferationresponses in female mice. Physiol Behav.1999;68(1-2):169-74.

  28. Sasaki S, Nagata K, Kobayashi Y.Regulation of the estrous cycle by neutrophilinfiltration into the vagina. BiochemBiophys Res Commun. 2009;382(1):35-40.

  29. McDonald TP, Swearingen CJ, CottrellMB, Clift RE, Bryant SE, Jackson CW. Sexandstrain-related differences in megakaryocytopoiesisand platelet productionin C3H and BALB/C mice. J Lab Clin Med.1992;120(1):168-73.

  30. Barrios M, Rodríguez A, Gil A, MSalazar A, Taylor P, E Sánchez E, et al. Comparativehemostatic parameters in BALB/c,C57BL/6 and C3H/He mice. Thromb Res.2009;124:338-43.

  31. Serfilippi LM, Pallman DR, Russell B.Serum clinical chemistry and hematologyreference values in outbred stocks of albinomice from three commonly used vendorsand two inbred strains of albino mice. ContempTop Lab Anim Sci. 2003;42:46-52.

  32. Fernández I, Peña A, Del Teso N,Rodríguez Cuesta J. Clinical biochemistryparameters in C57BL/6J mice after bloodcollection from the submandibular vein andretroorbital plexus. J Am Assoc Lab Anim Sci.2010;49:202-6.

  33. Coman C, Vlase E, Dinu A. Hematologicaland plasma biochemical values in fourspecific pathogen-free mice strains producedin the animal facility at Cantacuzino Institute,Bucharest. Scientific Works. Series C. VetMed. 2018;1:115-20.

  34. Gordon PO, Rathkolb B, OestereicherMA, Lengger Ch J, Moerth C, Micklich K, etal. Clinical chemistry reference intervals forC57BL/6J, C57BL/6N, and C3HeB/FeJ mice(Mus musculus). J Am Assoc Lab Anim Sci.2016;55:375-86.

  35. Santos EW, Cunha de Oliveira D, HastreiterA, Batista da Silva G, Soares de OliveiraBeltran J, Tsujita M, et al. Hematological andbiochemical reference values for C57BL/6,Swiss Webster and BALB/c mice. Braz J VetRes Anim Sci. 2016;53:138-45.

  36. Zhou X, Hansson GK. Effect of sex andage on serum biochemical reference ranges inC57BL/6J mice. Comp Med. 2004;54:176-8.

  37. Silva A, Guimarães AC, Nascimento D,Cota UA, Lazo JE. Valores de referência deparâmetros bioquímicos no sangue de duaslinhagens de camundongos. J Bras Patol MedLab. 2008;44:429-32.

  38. Charles River Laboratories. ResearchModels. C57BL/6 mice model informationsheet. c2022 [cited 2022 Jan 16]. Availablefrom: https://www.criver.com/sites/default/files/resources/doc_a/C57BL6MouseModelInformationSheet.pdf

  39. Charles River Laboratories. BALB/cmouse mice Clinical Pathology Data. 2012[cited 2022 Jan 16]. Available from: https://www.criver.com/sites/default/files/resources/doc_a/C57BL6MouseModelInformation-Sheet.pdf




2020     |     www.medigraphic.com