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2022, Number 2

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Rev Cubana Plant Med 2022; 27 (2)

Evaluation of angiogenic and embryotoxic activity of Anadenanthera peregrina extract (angico-do-Cerrado)

Augusto MT, Alves GR, Silva NP, de Souza LJR, de Melo RPR, Rodrigues FMM, Silva JNJ

Language: Spanish
References: 32
Page:
PDF size: 828.37 Kb.


ABSTRACT

Introduction: The genus Anadenanthera has been reported in the literature with antioxidant, anti-inflammatory, antimicrobial effect and healing action in the treatment of wounds.
Objective: To evaluate the in vivo angiogenic and embryotoxic activities of Anadenanthera peregrina extract (angico-do-cerrado).
Methods: Angiogenesis was performed in the chorioallantoic membrane of chicken embryo egg and zebrafish embryotoxicity.
Results: With respect to angiogenesis, a statistically significant difference (p<0.001) was observed between the test and control groups for all the parameters analyzed. Anadenanthera peregrina extract at EX2 (62 mg/ml) and EX3 (124 mg/mL) concentrations were angiogenic in comparisson to inhibition and induction control. For embryotoxicity, the mortality rate increased with increasing concentration, and an increase in dose and time-dependent embryotoxicity were observed. The lethal concentration (CL50) ranged from 0.331 mg/mL over the period from 24 hpf to 0.007 mg/ml to 168 hpf (Δ%=-97.9), decreasing with increasing exposure (p-value=0.001). Heart rate decreased progressively and significantly with increased concentration at all tested exposure times. Compared to control, it had a low hatching rate and no hatching of the 0.0605 mg/ml concentration for all periods.
Conclusions: It was evidenced that Anadenanthera peregrina extract has angiogenic activity as described for popular use in wound healing. However, embryotoxic effects were observed at high concentrations.


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