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2007, Number 1

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Rev Inst Nal Enf Resp Mex 2007; 20 (1)

Direct correlation between FEF25-75 and plasmatic paraoxonase activity in adult patients with asthma

Sierra VMP, Fernández VM, Torres RJD, Hicks GJJ

Language: Spanish
References: 24
Page: 9-14
PDF size: 116.26 Kb.


ABSTRACT

Introduction: Plasma myeloperoxidase activity (MPO) (EC 1.11.1.7) has been related to several chronic-degenerative diseases such as asthma, which have in common a chronic inflammatory process. Paraoxonase (PON-1) (EC 3.1.1.2), is an arylesterase enzyme that has a hydroperoxide catalytic function. This enzyme is a component of APO A-I located in HDL. PON1 is has been considered to protect lipoproteins, because it interrupts the oxidation process of LDL, conducive to the atherosclerotic process.
Objectives: To demonstrate that, a) oxidative stress in asthmatic patients correlates with the MPO activity, and b) demonstrate the role of PON1 activity as a biomarker of their pulmonary function.
Methods: The activity of both enzymes was measured in plasma of patients with asthma and compared to a control group of healthy subjects.
Results: In the group of patients with asthma, MPO activity increased 42% (57.31 ± 7.2 U vs 33.34 ± 4.7 U p‹0.05), while PON-1 activity decreased 52% (0.09 ± 0.1 nmol p-nitrophenol/mg prot vs 0.05 ± 0.01 nmol p-nitrophenol/mg prot p‹0.01). MPO activity showed an inverse correlation with FEV1 with a Spearman r of -0.57, while PON-1 activiy showed a direct correlation with FEF25-75, Spearman r of 0.64.
Conclusions: In this study we show, for the first time, that asthma patients, in whom there is a state of oxidative stress that affects the pulmonary function, PON-1 determination can be useful as a predictor of a better pulmonary function, whereas an increment in MPO activity could be associated to an acute inflammatory process, implicating cellular damage.
* U = U/mg of protein-1.


REFERENCES

  1. Salako, BL, Ajayi, SO. Bronchial asthma: a risk factor for hypertension? Afr J Med Sci 2000;29:47-50.

  2. De Catherina R, Zampolli A. From asthma to Atherosclerosis 5-Lipoxygenase, leukotrienes and inflammation. N Engl J Med 2004;350(1):4-7.

  3. Wannamethee G, Shaper AG, Ebrahim S. Respiratory function and risk of stroke. Stroke 1995;26:2004-2001.

  4. Barnes PJ. Reactive oxigen species and airway inflammation. Free Radic Biol Med 1990;9:235-43.

  5. Nadeem A, Chhabra SK, Masood A, Raj HG. Increased oxidative stress and altered levels of antioxidants in asthma. J Allergy Clin Immunol 2003;111(1):72-8.

  6. Monteseirin J, Bonilla I, Camacho J, Conde J, Sobrino F. Elevated secretion of myeloperoxidase by neutrophils from asthmatic patients: the effect of immunotherapy. J Allergy Clin Immunol 2001;107(4):623-6.

  7. Abul H, Abul A, Khan I, Mathew TC, Ayed A, Al-Athary E. Levels of IL-8 and myeloperoxidase in the lungs of pneumonia patients. Mol Cell Biochem 2001;217(1-2):107-12.

  8. Mathy-Hartert M, Damas P, Nys M, et al. Nitrated proteins in bronchoalveolar lavage fluid of patients at risk of ventilator-associated bronchopneumonia. Eur Respir J 2000;16(2):296-301.

  9. Cantin AM, North SL, Fells GA, Hubbard RC, Crital RG. Oxidant-mediated epithelial cell injury in idiopathic pulmonary fibrosis. J Clin Invest 1987;79:1665-1673.

  10. Ricciardolo FL, Caramori G, Ito K, et al. Nitrosative stress in the bronchial mucosa of severe chronic obstructive pulmonary disease. J Allergy Clin Immunol 2005;116(5):1028-35.

  11. Keatings VM, Barnes NC. Granulocyte activation markers in induced sputum: comparisons between chronic obstructive pulmonary disease, asthma, and normal subjects. Am J Respir Crit Care Med 1997; 155:449-453.

  12. Vita JA, Brennan M-L, Gokce Noyan, Mann SA, Goormastic M, Shishehbor. Serum Myeloperoxidase Levels Independently Predict Endothelial Dysfunction in Humans. Circulation 2004;110:1134-1139.

  13. Baldus S, Heeschen C, Meinertz T, Zeiher AM, Eiserich JP, Münzel T, et al. Myeloperoxidase Serum Levels Predict Risk in Patients With Acute Coronary Syndromes. Circulation 2003;108:1440-1445.

  14. National Asthma Education and Prevention Program. Expert panel report: guidelines for the diagnosis and management of asthma: update on selected topics-2002. Bethesda, Md.: U.S. Department of Health and Human Services, Public Health Service, National Institutes of Health, National Heart, Lung, and Blood Institute, 2003; NIH publication no. 02-5074.

  15. American Thoracic Sociert. Standarization of spirometry. 1994 update. Am J Respir Crit Care Med 1995; 152:1107-1136.

  16. Bozeman PC, et al. Assay of the human leukocyte enzymes myeloperoxidase and eosinophil peroxidase. Journal of Immunological Methods 1960;126:125-133.

  17. Abbott CA, Mackness MI, Kumar S, Boulton AJ, Durrington PN. Serum paraoxonase activity, concentration and phenotype distribution in diabetes mellitus and its relationship to serum lipids and lipoproteins. Arteriosclerosis, Thrombosis and Vascular Biology 1995;15:1812-1818.

  18. Tsukagoshi H, Robbins RA, Barnes PJ, Cheng KF. Role of nitric oxide and sueroxide anion in interleukin-1b-induced airway hyperresponsiveness to bradykinin. Am J Respir Crit Care Med 1994;150:1019-1025.

  19. Asano T, Hattori T, Tada T, Kajikuri J, Kamiya T, Saitoh M, Yamada Y, Itoh M, Itoh T. Role of the epithelium in opposing H2O2-induced modulation of acetylcholine-induced contractions in rabbit intrapulmonary bronchiole. Br J Pharmacol 2001;132:1271-1280.

  20. Jarjour NN, Calhoum WJ. Enhanced production of oxygen radicals in asthma. J Lab Clin Med 1994; 123: 131-137.

  21. Antczak A, Nowak D, Shariati B, Krol M, Piasecka G, Kurmanowska Z. Increased hydrogen peroxide and thiobarbituric acid-reactive products in expired breath condensate of asthmatic patients. Eur Respir J 1997; 10:1235-1241.

  22. Olopade CO, Zakker M, Swedler WI, Rubenstein I. Exhaled pentane levels in acute asthma. Chest 1997; 111:862-865.

  23. Montuschi P, Corradi M, Ciabattoni G, Nightingale J, Kharitonov SA, Barnes PJ. Increased 8 isoprostane, a maker of oxidative stress, in exhaled condensate of asthma patients. Am J Respir Crit Care Med 1999; 160:216-220.

  24. Sierra-Vargas MP, Torres-Ramos YD, Bernabé-Cabanillas RM, Blanco-Jimenez S, Ceballos G, Hicks JJ. Air pollutant material from three different places (Mexico City) increase free radicals generation in vitro by circulating leukocytes from asthmatics. Enviado para publicación. Environ Health Perspectives.




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