Castaño I, Cormack B, De Las Peñas A

Language: Spanish
References: 22
Page: 66-69
PDF size: 86.60 Kb.

ABSTRACT

Candida glabrata is an opportunistic fungal pathogen that has become increasingly frequent in bloodstream and mucosal infections in immunocompromised patients. C. glabrata is phylogenetically more closely related to S. cerevisiae than to C. albicans, and some well identified virulence factors in C. albicans do not seem to be conserved in C. glabrata. However, other important traits are shared by both organisms, and these may play a role in the adaptation and survival in the host as opportunistic pathogens. Both species adhere tightly to host cells, and C. glabrata has a large family of subtelomeric genes encoding cell surface proteins that mediate this adherence. Expression of these genes is regulated by a chromatin-based negative regulation termed subtelomeric silencing. C. albicans also possesses several adhesins although they are not regulated by this mechanism.
C. albicans and C. glabrata have been considered asexual, but recent work has demonstrated the existence of a cryptic sexual cycle in C. albicans. The fact that C. glabrata contains all of the genes essential for mating suggests the possibility that C. glabrata might also have a tightly regulated sexual cycle. Both organisms can form biofilms and can undergo phenotypic switching which could be important for rapid adaptation to the changing environmental conditions encountered in the host as opportunistic pathogens.

REFERENCES

1. Almirante, B., Rodriguez, D., Park, B.J., Cuenca-Estrella, M.,Planes, A.M., Almela, M., Mensa, J., Sanchez, F., Ayats, J., Gimenez, M., Saballs, P., Fridkin, S.K., Morgan, J., Rodriguez-Tudela, J.L., Warnock, D.W., and Pahissa, A. (2005) Epidemiology and predictors of mortality in cases of Candida bloodstream infection: results from population-based surveillance, barcelona, Spain, from 2002 to 2003. J Clin Microbiol 43: 1829-1835.

2. Buitron Garcia, R., Romero Cabello, R., Cruz Talonia, F., Bonifaz, A., and Zarama Marquez, F. (2002) [Study on Candida no-albicans species and its relation to recurrent vulvovaginal candidiasis]. Ginecol Obstet Mex 70: 431-436.

3. Byrne, K.P., and Wolfe, K.H. (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15: 1456-1461.

4. Castaño, I., Pan, S.J., Zupancic, M., Hennequin, C., Dujon, B., and Cormack, B.P. (2005) Telomere length control and transcriptional regulation of subtelomeric adhesins in Candida glabrata. Mol Microbiol 55: 1246-1258.

5. Csank, C., and Haynes, K. (2000) Candida glabrata displays pseudohyphal growth. FEMS Microbiol Lett 189: 115-120.

6. De Las Peñas, A., Pan, S.J., Castaño, I., Alder, J., Cregg, R., and Cormack, B.P. (2003) Virulence-related surface glycoproteins in the yeast pathogen Candida glabrata are encoded in subtelomeric clusters and subject to RAP1- and SIR-dependent transcriptional silencing. Genes Dev 17: 2245-2258.

7. Domergue, R., Castano, I., De Las Penas, A., Zupancic, M., Lockatell, V., Hebel, J.R., Johnson, D., and Cormack, B.P. (2005) Nicotinic acid limitation regulates silencing of Candida adhesins during UTI. Science 308: 866-870.

8. Hull, C.M., Raisner, R.M., and Johnson, A.D. (2000) Evidence for mating of the “asexual” yeast Candida albicans in a mammalian host. Science 289: 307-310.

9. Iraqui, I., Garcia-Sanchez, S., Aubert, S., Dromer, F., Ghigo, J.M., d’Enfert, C., and Janbon, G. (2005) The Yak1p kinase controls expression of adhesins and biofilm formation in Candida glabrata in a Sir4p-dependent pathway. Mol Microbiol 55: 1259-1271.

10. Johnson, A. (2003) The biology of mating in Candida albicans. Nat Rev Microbiol 1: 106-116.

11. Kantarcioglu, A.S., and Yucel, A. (2002) Phospholipase and protease activities in clinical Candida isolates with reference to the sources of strains. Mycoses 45: 160-165.

12. Krcmery, V. (1999) Torulopsis glabrata an emerging yeast pathogen in cancer patients. Int J Antimicrob Agents 11: 1-6.

13. Lachke, S.A., Srikantha, T., Tsai, L.K., Daniels, K., and Soll, D.R. (2000) Phenotypic switching in Candida glabrata involves phase-specific regulation of the metallothionein gene MT-II and the newly discovered hemolysin gene HLP. Infect Immun 68: 884-895.

14. Lachke, S.A., Joly, S., Daniels, K., and Soll, D.R. (2002) Phenotypic switching and filamentation in Candida glabrata. Microbiology 148: 2661-2674.

15. Magee, B.B., and Magee, P.T. (2000) Induction of mating in Candida albicans by construction of MTLa and MTLalpha strains. Science 289: 310-313.

16. Manzano-Gayosso, P., Hernandez-Hernandez, F., Bazan-Mora, E., Mendez-Tovar, L.J., Gonzalez-Monroy, J., and Lopez-Martinez, R. (2000) [Identification and typing of yeast isolates from hospital patients in Mexico City]. Rev Argent Microbiol 32: 1-6.

17. Pfaller, M.A., and Diekema, D.J. (2004) Twelve years of fluconazole in clinical practice: global trends in species distribution and fluconazole susceptibility of bloodstream isolates of Candida. Clin Microbiol Infect 10 Suppl 1: 11-23.

18. Rusche, L.N., Kirchmaier, A.L., and Rine, J. (2003) The establishment, inheritance, and function of silenced chromatin in Saccharomyces cerevisiae. Annu Rev Biochem 72: 481-516.

19. Srikantha, T., Lachke, S.A., and Soll, D.R. (2003) Three mating type-like loci in Candida glabrata. Eukaryot Cell 2: 328-340.

20. Trick, W.E., Fridkin, S.K., Edwards, J.R., Hajjeh, R.A., and Gaynes, R.P. (2002) Secular trend of hospital-acquired candidemia among intensive care unit patients in the United States during 1989-1999. Clin Infect Dis 35: 627-630.

21. Wolfe, K.H. (2006) Comparative genomics and genome evolution in yeasts. Philos Trans R Soc Lond B Biol Sci 361: 403-412.

22. Wong, S., Fares, M.A., Zimmermann, W., Butler, G., and Wolfe, K.H. (2003) Evidence from comparative genomics for a complete sexual cycle in the ‘asexual’ pathogenic yeast Candida glabrata. Genome Biol 4: R10.