Cubas-Mogollón JW, Jiménez-Sánchez SM, Ruiz-Ramírez E, Erazo-Paredes CH, Aguirre-Siancas EE

Language: English
References: 33
Page: 43-54
PDF size: 737.58 Kb.

ABSTRACT

The aim of this experimental study was to determine the effect of photobiomodulation therapy on bone repair in a rat tibia osteotomy model at 15 and 30 days. The sample consisted of 36 male Holtzman rats that were randomized into 6 equal groups. Groups A1 and A2: osteotomy + 1 J laser energy. Groups B1 and B2: osteotomy + 3 J laser energy. Groups C1 and C2 (controls): osteotomy only. The bone repair was analyzed by histological evaluation of osteoblasts and osteocytes both at 15 days (groups A1, B1, and C1) and at 30 days (groups A2, B2, and C2). Within the results, in all groups a greater number of osteoblasts was found at 15 days vs 30 days (p‹0.05), and a greater number of osteocytes in B1 and C2 vs B2 and C1, respectively (p‹0.05). When evaluating the 3 groups worked up to 15 days, more osteoblasts were found in A1 and C1 vs B1 (p‹0.001); and osteocytes predominated in A1 and B1 vs C1 (p‹0.001). At 30 days there was a greater quantity of osteoblasts in C2 vs A2 and B2 (p‹0.05) and of osteocytes in C2 vs B2 (p‹0.05). It is concluded that 1 J photobiomodulation therapy improved bone repair at 15 days; however, this improvement was not observed at 30 days because there were no differences between the irradiated groups and the control.

REFERENCES

1. Hosseinpour S., Fekrazad R., Arany P., Ye, Q.Molecular impacts of photobiomodulation onbone regeneration: A systematic review. ProgBiophysd Mol Biol. 2019; 149: 147-159

2. Nica D., Heredea E., Todea D. Alveolussoft and bone tissue regeneration after laserbiomodulation - a histological study. Rom JMorphol Embryol. 2019; 60 (4): 1269-1273.

3. Karu T.I. Multiple roles of cytochrome coxidase in mammalian cells under action ofred and IR-A radiation. IUBMB Life. 2010;62 (8): 607-610.

4. De Freitas L., Hamblin M. Proposed Mechanismsof Photobiomodulation or Low-LevelLight Therapy. IEEE J Sel Top QuantumElectron. 2016; 22 (3): 7000417.

5. Hamblin M. Mechanisms and applicationsof the anti-inflammatory effects of photobiomodulation.AIMS biophysics. 2017; 4 (3):337-361.

6. Ustaoglu G., Ercan E., Tunali, M. Low-levellaser therapy in enhancing wound healingand preserving tissue thickness at free gingivalgraft donor sites: a randomized, controlledclinical study. Photomed Laser Surg.2017; 35: 223-230.

7. Dostalova T., Kroulikova V., PodzimekS., Jelinkov H. Low-level laser therapyafter wisdom teeth surgery: evaluation ofimmunologic markers (secretory immunoglobulinand lysozyme levels) and thermographicexamination: placebo controlled study.Photomed Laser Surg. 2017; 35: 616-621.

8. Escudero J., Perez M., de Oliveira Rosso M.,Buchaim V., Pomini K., Campos L., AudiM., Buchaim R. Photobiomodulation therapy(PBMT) in bone repair: A systematic review.Injury. 2019; 50 (11): 1853-1867.

9. Hanna R., Dalvi S., Amaroli A., De AngelisN., Benedicenti S. Effects of photobiomodulationon bone defects grafted with bonesubstitutes: A systematic review of in vivoanimal studies. J Biophotonics. 2021; 14 (1):e202000267.

10. Chumpitaz Cerrate V., Franco Quino C.,Aguirre Siancas E. Influence of ambientoxygen pressure on guided bone regeneration.Rev. Clin. Periodoncia Implantol.Rehabil. Oral. 2017; 10 (2): 111-114.

11. Setiawati R., Rahardjo P. Bone Developmentand Growth. In Osteogenesis and BoneRegeneration. Londres: IntechOpen; 2018.

12. De Almeida J., De Moraes R., Gusman D.,Faleiros P., Nagata M., Garcia V., TheodoroL., Bosco A. Influence of low-level lasertherapy on the healing process of autogenousbone block grafts in the jaws of systemicallynicotine-modified rats: A histomorphometricstudy. Arch Oral Biol. 2017; 75: 21-30.

13. Zaky A., El Shenawy H., Harhsh T., ShalashM., Awad N. Can Low Level Laser TherapyBenefit Bone Regeneration in LocalizedMaxillary Cystic Defects? - A ProspectiveRandomized Control Trial. Open accessMaced J Med. 2016; 4 (4): 720-725.

14. Amaroli A., Agas D., Laus F., Cuteri V.,Hanna R., Sabbieti M., Benedicenti S. TheEffects of Photobiomodulation of 808 nmDiode Laser Therapy at Higher Fluence onthe in Vitro Osteogenic Differentiation ofBone Marrow Stromal Cells. Front Physiol.2018; 9: 123.

15. Hanna R., Agas D., Benedicenti S., FerrandoS., Laus F., Cuteri V., Lacava G., Sabbieti M.,Amaroli A. A Comparative Study Betweenthe Effectiveness of 980 nm PhotobiomodulationDelivered by Hand-Piece WithGaussian vs. Flat-Top Profiles on OsteoblastsMaturation. Front Endocrinol (Lausanne).2019; 10: 92.

16. De Paiva P., Casalechi H., Tomazoni S.,Machado C., Miranda E., Ribeiro N., PereiraA., da Costa A., Dias L., Souza B., AguiarLino M., de Carvalho P. & Leal-Junior E.Effects of photobiomodulation therapy inaerobic endurance training and detraining inhumans: Protocol for a randomized placebocontrolledtrial. Medicine (Baltimore). 2019;98 (18): e15317.

17. Jenkins P., Carroll J. How to ReportLow-Level Laser Therapy (LLLT)/PhotomedicineDose and Beam Parameters in Clinicaland Laboratory Studies. Photomed LaserSurg. 2011; 29 (12): 785-787.

18. Llapapasca Cruz C., De la Torre F., JiménezSánchez S., Mallma Medina A., Ruiz RamirezE., Valdez Jurado F. Efecto del láser terapéuticoinfrarrojo en la reparación ósea postexodonciaen ratas albinas. Rev. Estomatol.Herediana. 2017; 27 (2): 101-110.

19. Atasoy K.T., Korkmaz Y.T., Odaci E., HanciH. The efficacy of low-level 940 nm lásertherapy with different energy intensities onbone healing. Braz. Oral Res. 2017; 31: 1-9.

20. Çırak E., Özyurt A., Peker T., ÖmeroğluS., Güngör M. Comparative evaluation ofvarious low-level laser therapies on bonehealing following tooth extraction: Anexperimental animal study. J CraniomaxillofacSurg. 2018; 46 (7): 1147-1152.

21. Desai S., Mudda J., Patil V., Maharudrappa,Satish B. Effect of irradiation of 810nmlaser on bone for 10 sec: A rabbit histologicalstudy. IOSR-JDMS. 2019; 18 (3): 14-20.

22. Abdel Hamid M., Zaied A., Zayet M., AbdelmageedH., Hassan E., Amaroli A. Efficacyof Flat-Top Hand-Piece Using 980 nm DiodeLaser Photobiomodulation on Socket Healingafter Extraction: Split-Mouth ExperimentalModel in Dogs. Photochem Photobiol. 2020;97: 627-633.

23. Brito G., Macedo F., Moura J., de CarvalhoM. Effectiveness of gallium and aluminumArsenide laser in bone repair. BJHR. 2021; 4(2): 5301-5315.

24. Tim C.; Bossini P.; Kido H., Malavazi I., VonZeska Kress M.; Carazzolle M., Parizotto N.,Rennó A. Effects of low-level laser therapyon the expression of osteogenic genes duringthe initial stages of bone healing in rats: amicroarray analysis. Lasers Med Sci. 2015;30 (9): 2325-2333.

25. Carneiro V.S.M., Jr. F.dA.L., Gerbi M.E.M.,Menezes R.F.d., SantosNeto A.Pd., AraújoN.C. Diode λ830nm laser associated withhydroxyapatite and biological membranes:bone repair in rats. 2016. Proc. SPIE 9692,Lasers in Dentistry XXII.

26. Fernandes K.R., Magri A.M.P., Kido H.W.,et al. Biosilicate/PLGA osteogenic effectsmodulated by laser therapy: in vitro and invivo studies. J Photochem Photobiol B. 2017;173: 258-265.

27. Gabbai-Armelin P.R., Caliari H.M., SilvaD.F., et al. Association of Bioglass/collagen/magnesium composites and low level irradiation:effects on bone healing in a model of tibialdefect in rats. Laser Ther. 2018; 27 (4): 271-282.

28. De Oliveira G., Aroni M., Medeiros M.,Marcantonio E., Marcantonio R. Effect oflow-level laser therapy on the healing of sitesgrafted with coagulum, deproteinized bovinebone, and biphasic ceramic made of hydroxyapatiteand β-tricalcium phosphate. In vivostudy in rats. Lasers Surg. Med. 2018; 50 (6):651-660.

29. Yılmaz B., Akman A., Çetinkaya A., ColakC., Yildirim B., Yucel O., Guncu G. &Nohutcu R. In vivo efficacy of low-levellaser therapy on bone regeneration. LasersMed Sci. 2022; 37: 2209-2216

30. Chumpitaz-Cerrate V., Chávez-RimacheL., Franco-Quino C., Aguirre-Siancas E.,Caldas-Cueva V., Ruiz Ramirez, E. Effects ofNSAIDs and environmental oxygen pressureon bone regeneration. J Oral Res 2019; 8 (2):152-158.

31. Esteves J.C., de Souza Faloni A.P., MacedoP.D., Nakata P.B., Chierici MarcantonioR.A., Intini G, Marcantonio E. Effects onBone Repair of Osteotomy With Drills orWith Erbium, Chromium: Yttrium-Scandium-Gallium-Garnet Laser: Histomorphometricand Immunohistochemical Study. J Periodontol.2016, 87 (4): 452-460.

32. Chumpitaz-Cerrate V., Chávez-Rimache L.,Aguirre-Siancas E., Franco-Quino C., Ruiz-Ramirez E., Paredes-Erazo C. Biochemicaland histopathological effects of diclofenacand ketoprofen administration onbone regeneration. Braz Dent Sci. 2021; 24(3): 1-8.

33. Chumpitaz-Cerrate V., Chávez-Rimache L.,Franco-Quino C., Ruiz Ramirez, E., Aguirre-Siancas E., Paredes Erazo C. Effects ofsalbutamol, montelukast and prednisone onorthodontic tooth movement in rats. BrazDent Sci. 2021; 24 (2): 1-9.