Martínez-Ramos A, Isibasi A

Language: Spanish
References: 23
Page: 97-101
PDF size: 82.21 Kb.

ABSTRACT

Background. The lipopolysaccharide (LPS) of several Gram negative bacteria have influence on their virulence and on the normal human serum (NHS) susceptibility or resistance. Among several biological roles the LPS has showed to protect some Gram negative bacteria from being killed by NHS. Objective. Purify the LPSs of two strains of Klebsiella pneumoniae a serum susceptible one and another serum resistant one. Attempts to make an analysis of their chemical composition and the influence that the purified products have on the lethal activity of the SHN were carried out. Material and methods. The LPS of both bacterial strains were purified and their content in carbohydrates, KDO and fatty acids as well as their influence against the bactericidal effect of NHS, were determined. Results. In relation with their chemical composition small differences were determined for both purified LPSs which were more quantitative than qualitative. The purified LPSs showed a 100% protective effect against bactericidal activity of NHS. Conclusions. The chemical composition of purified LPSs had a high grade of similarity. Purified LPSs protected serum susceptible bacterial strain from being killed by NHS.

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