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Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2004, Number 3

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Rev Mex Patol Clin Med Lab 2004; 51 (3)

Score regions of nucleolar organizers NORs in the epithelial lesions of buccal cavity

Leyva HER, Zárate DAN, Ramírez MGM, Vega ME

Language: Spanish
References: 16
Page: 145-152
PDF size: 98.43 Kb.


ABSTRACT

Objective: The aim of the study was to evaluate NORs’ number in: common wart, frictional keratosis, epithelial dysplasia, lichen planus, squamous cell carcinoma and basal cell carcinoma. Methods: 68 cases were histopathologically analyzed by hematoxylin and eosin technique and the AgNOR technique described by Ploton, using silver nitrate. Results: NORs average was: common wart 9 ± 2.24, frictional keratosis 5.5 ± 1.16, epithelial dysplasia 8 ± 2.12, lichen planus 6.8 ± 2.13, squamous cell carcinoma 6.96 ± 0.53 and basal cell carcinoma 3.55 ± 0.25. There was a relation between AgNORs’ number and dysplasia rs = 0.9, with a reliable level of p = 0.05. The relation between the average of nucleolar organizers in lichen planus and in squamous cell carcinoma was rs = 0.99 with a reliable level of p = 0.1. Between basal cell and squamous cell carcinomas, the relation was rs = 1.35 with a reliable level of p = 0.05. Conclusions: The grade of basal cell and squamous cell carcinomas’ malignity and the number of nucleolar organizers are positively related, meaning that as organizers’ number increases, malignity grade does.


REFERENCES

  1. Warnakulasuriya K. Johnson N. Nucleolar organizer region (Nor) distribution as a diagnostic marker in oral keratosis, dysplasia and squamous cell carcinoma. J Oral Pathol Med 1993; 22: 77-81.

  2. Karp G. Biología celular. 2a ed. México: Mc Graw-Hill, 1987; 522-723.

  3. Donofrio V, Lo Muzio, Mignogna G, Troncone S, Staibano A, et al. Prognostic evaluation of HPV-Associated precancerous and microinvasive carcinoma of the Oral Cavity: Combined use of nucleolar organizer regions (AgNOR) and proliferating cell nuclear antigen (PCNA). Oral Oncol Eur J Cancer 1995; 31B (3): 174-180.

  4. Schwint AE, Savino TM, Lanfranchini HE, Marschoff E, Cabrini RL, Itoiz ME. Nucleolar organizer regions in lining epithelium adjacent to squamous cell carcinoma or human oral mucosa. Cancer 1994; 73 (11): 2674-2679.

  5. Xie X, Frass COP, Subdo J, Boysen M. Diagnostic and prognostic value of nucleolar organizer regions in normal epithelium, dysplasia and squamous cell carcinoma of the oral cavity. Am Cancer Soc 1997; 79: 2200-2208.

  6. Cabrini RL, Schwint AE, Mendez A, Femopase F, Lanfranchini H, Itoiz ME. Morphometric study of nucleolar organizer regions in human oral normal mucosa, papilloma and squamous cell carcinoma. J Oral Pathol Med 1992; 21: 275-279.

  7. Masanori S, Seiji N, Takeshi H, Makoto S, Masuichiro O. Mode of tumor invasion in oral squamous cell carcinoma: improved grading based on extracellular matrices. Head and Neck 1996; 80: 55-62.

  8. Leyva HER, Flores G, Barrera R, Aldape BB. Regiones de organizadores nucleolares y examen por PCR de RNAr 18s en 19 casos de carcinoma epidermoide de cavidad bucal. Rev Div Estud Posgrado Invest Fac Odont UNAM 1998; 7: 17-25.

  9. Migaldi M, Criscuolo M, Zunarelli E, Lo Branco L, Martinelli AM, Barbolini G. P 120 and AgNOR nucleolar protein expression. Oral Surg, Oral Med, Oral Pathol, Oral Radiol, Endod 1998; 85: 189-196.

  10. Derenzani M, Sirri V, Trere D. Nucleolar organizer regions in tumor cells. Cancer J 1997; 7 (2): 1-10.

  11. Rosa G. Prognostic evaluation of HPV associated precancerous and microinvasive carcinoma of the oral cavity: Combined use of nucleolar organizer regions (AgNOR) and proliferating cell nuclear antigen (PCNA). Oral Oncol Eur J Cancer 1995; 31B (3): 174-180.

  12. Schwint AE, Gómez E, Itoiz ME, Cabrini RL. Nucleolar organizer regions as markers of incipient cellular alterations in squamous epithelium. J Dent Res 1993; 72 (8): 1233-1236.

  13. Arora HL, Arora N, Solanki RL. Argyrophilic nucleolar organizer regions in soft tissue tumors. Indian Pathol Microbiol 1996; 39 (4): 257-263 (Abstract).

  14. Chatopadhyay A, Chawda JG, Doshi JJ. Silver binding nucleolar organizing region: A study of oral leukoplakia and squamous cell carcinoma. Int J Oral Maxillofacial Surg 1994; 23: 374-377.

  15. Chatterjee R, Mukhopadhyay D, Chakraborty RN, Mitra RB. Evaluation of argyrophilic nucleolar organizer regions (AgNORs) in oral carcinomas in relation to human papillomavirus infection and cytokinetics. J Oral Pathol Med 1997; 26 (7): 310-314 (Abstract).

  16. De Rosa G, Staibano S, Barra E, Zeppa P, Salvatore G, Vetrani A, Palombini L. Nucleolar Organizer Region in Aggresive and Nonaggressive Basal Cell Carcinoma of the Skin. Cancer 1992; 69: 123-126.




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