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2022, Number 3

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Rev Med UAS 2022; 12 (3)

Kaempferol induces prooxidant effect and ultrastructural changes as amoebicidal mechanism

Jarillo-Luna RA, Gutiérrez-Meza JM, Barbosa-Cabrera E, Luna-Palencia GR, Rosales-Hernández MC, Nequiz M, Pacheco-Yepez J

Language: Spanish
References: 33
Page: 195-205
PDF size: 377.24 Kb.


ABSTRACT

Objective: Perform a comparative in vitro analysis of the anti-amoebic activity of the flavonoid Camperol compared to the reference drug, Metronidazole and determine if the damage mechanism is oxidative. Methodology: Different concentrations of Camperol and Metronidazole were applied to Entamoeba histolytica trophozoites, analyzing by electron microscopy the structural damage caused, by colorimetric methods the anti- amoebic effect with and without oxygenation conditions, the DNA damage by fluorometric method, the cytotoxicity of Camperol in HeLa cells by colorimetric method and the production of superoxide anion by electron paramagnetic resonance. Results: The degree of amoebic destruction depends on the time and concentration of the flavonoid. Camperol showed anti-amoebic activity against Entamoeba histolytica similar to Metronidazole; induced ultrastructural changes in the cytoplasm of Entamoeba histolytica trophozoites, and alteration of the cell membrane, induced DNA damage, showed a prooxidant effect and maintained its anti-amoebic activity in a highly oxygenated medium. Finally, Camperol did not show toxicity against HeLa cells. Conclusions: The anti-amoebic effects are due to the pro-oxidant characteristics of Camperol, an effect that exceeds the anti-oxidant mechanisms of the amoeba, suggesting that in vivo it would be a better amebicide than Metronidazole.


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