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2020, Number 3

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Rev Cub Gen 2020; 13 (3)

Dynamics of filial relationship studies in Cuba

Collazo MT, Hernández PY, Gómez MM, Juárez FR, Machado MD, Merencio SL, Ferreira CR

Language: Spanish
References: 24
Page: 1-20
PDF size: 796.59 Kb.


ABSTRACT

Introduction: Uncertainty about filial relationships has psico-social effects on people involved, and at present it is possible to be resolved using DNA markers. These kinds of studies are being developed since 2017 thanks to the collaboration between Cubans National Centre for Medical Genetics and Legal Medicine Institute.
Objectives: Knowing the main characteristics of filial relationships studies developed in Cuba during 2017 and 2018 in order to draw up an approach for long-term continuity.
Methods: One hundred relationship studies, involving 115 filial relationship analyses, were resolved using 11 STR tipe DNA markers, which were genotyped through Polymerase Chain Reaction, denaturant polyacrylamide gel electrophoresis and silver staining. The proposal goals were to obtain at least a Paterninity Index equal or higher than 100 or no less than 3 exclusions. Multiplex system GenomeLab™ Human STR Primer Set (Beckman Coulter) was used in some studies that not achieved closing parameters or where rare genetic events were presented.
Results: Paternity studies were 98 % of total. Other kinds of studies were maternity test and siblings test (one case each one). One hundred fifteen filial relationships were analyzed, 112 of them being paternity relationships. Paternity analysis including mother were 76.8 % of them. Paternity was excluded in 33 % of paternity analysis, being 4 the average number of exclusion markers. 96.4 % of paternity analysis matched quality parameters concerning close standards.


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