medigraphic.com
ISSN 1027-2852 (Electronic)
ISSN 0864-4551 (Print)

2022, Number 1

<< Back

Biotecnol Apl 2022; 39 (1)

Discovery of ferrochelatase 1 in Nicotiana tabacum L.: Its role in the response to abiotic stress and plant development

Ortega-Rodés P, Grimm B, Hedtke B, Ortega DE, Fan T, Rodés GR, Pernús AM, Hey D, FGL, Brings L, Roling L, Schnurrer F, Meiers A, Alawady A

Language: English
References: 35
Page: 1511-1516
PDF size: 606.67 Kb.


ABSTRACT

Cuba is threatened by climate change. Some enzymes act as a shield against oxidative stress, always associated with salinity, water deficit, or excess and extreme temperatures; those enzymes have Heme as a cofactor. Heme is a tetrapyrrole, similar to chlorophyll, but with Fe instead of Mg as the central atom. The response associated with Heme was investigated in tobacco plants under saline, water, and temperature stress, together with the model plant Arabidopsis thaliana. The presence of the enzyme Ferrochelatase 1 (responsible for chelating iron to tetrapyrrole) was reported in tobacco tissues for the first time for science. Plants have two isoforms of Ferrochelatases (FC1 and FC2). Mutating the model plant for the Ferrochelatase 1 gene was lethal for the embryos. By comparing FC1 and FC2 mRNA levels in wild-type plant embryos, it was demonstrated that FC1 is the predominant isoform during embryo maturation. Therefore, the regulation of FC expression in time and space during embryogenesis is a prerequisite for the proper development of the embryo. It is concluded that the Heme produced by FC1 is essential for embryogenesis in addition to stress responses. Antisense lines for FC1 had an early flowering phenotype on short days; this made it possible for the first time to establish a relationship between the Heme produced by FC1 and the flowering process. Heme produced by the FC1 isoform is important in reproductive metabolism. This knowledge in the hands of breeders could be used to obtain productive cultivars with the capacity to acclimatize to abiotic stresses associated with climate change, which would potentially give us some advantage to mitigate its effects. This work has stimulated scientific exchange with Germany. The results are included as pedagogical techniques for the teaching of modern molecular biology methods, in the high-level training of young Cuban and Latin American scientists at the University of Havana, in the 5 editions of the joint course “Basic methods in plant molecular biology and plant physiology” with the Humboldt University of Berlin. This work received the Annual Award of the Cuban Academy of Sciences for the year 2020.


REFERENCES

  1. González Núñez LM, Tóth T, García D.Integrated management for the sustainableuse of salt-affected soil in Cuba. Universidady Ciencia. 2004;20(40):85-102.

  2. García M, Díaz AL, Valdés MA. Elmejoramiento de los suelos: una experienciadesde la agroecología en laCooperativa de Producción Agropecuaria“Celso Maragoto Lara”. Revista Científica.2014;16(4):317-28.

  3. Ortega F, Obregon A, Hernández A,Borreto M, editors. Los suelos salinos ysalinizados de Cuba. Resúmenes de lasActas del Primer Seminario Científico dePedología para la región de Centroaméricay el Caribe: Suelo y Agua; La Habana;1985.

  4. Roháček K, Soukupová J, Barták M.Chlorophyll fluorescence: A wonderful toolto study plant physiology and plant stress.In: Schoefs B, editor. Plant Cell Compartments.Kerala: Research Signpost; 2008;p. 41-104.

  5. Tanaka R, Tanaka A. Tetrapyrrole biosynthesisin higher plants. Annu Rev PlantBiol. 2007;58:321-46.

  6. Fan T, Grimm B, Layer G. Porphyrin andheme synthesis. Adv Bot Res. 2019;91:89-131.

  7. Woodson JD, Perez-Ruiz JM, Chory J.Heme synthesis by plastid FerrochelataseI regulates nuclear gene expression inplants. Curr Biol. 2011;21:897-903.

  8. Papenbrock J, Mishra S, Mock H-P,Kruse E, Schmidt EK, Petersmann A, et al.Impaired expression of the plastidic ferrochelataseby antisense RNA synthesis leadsto a necrotic phenotype of transformedtobacco plants. Plant J. 2001;28:41-50.

  9. Hoagland DR, Arnon DI. The waterculturemethod for growing plants withoutsoil. California Agricultural ExperimentStation Circular. 1950;347:1-32.

  10. Sharma P, Bhardwaj R, Arora N,Arora HK. Effect of 28-homobrassinolideon growth, Zn metal uptake and antioxidativeenzyme activities in Brassicajuncea L. seedlings. Braz J Plant Physiol.2007;19:203-10.

  11. Kato M, Shimizu S. Chlorophyll metabolismin higher plants. VII. Chlorophylldegradation in senescing tobacco leaves;phenolic- dependent peroxidative degradation.Can J Bot. 1987;65:729-35.

  12. Papenbrock J, Mock H-P, Kruse E,Grimm B. Expression studies in tetrapyrrolebiosynthesis: inverse maxima ofmagnesium chelatase and ferrochelataseactivity during cyclic photoperiods. Planta.1999;208:264-73.

  13. Edwards K, Johnstone C, Thompson C.A simple and rapid method for the preparationof plant genomic DNA for PCR analysis.Nucleic Acids Res. 1991;19(6):1349.

  14. Schmittgen TD, Livak KJ. Analyzingreal-time PCR data by the comparative CTmethod. Nat Protocols. 2008;3(6):1101-8.

  15. Livak KJ, Schmittgen TD. Analysis of relativegene expression data using real-timequantitative PCR and the 2(-Delta DeltaC(T)) method. Methods. 2001;25(4):402-8.

  16. Horsch RB, Fry JE, Hoffman NL, EichholtzSG, Rogers SG, Fraley RT. A simpleand general method for transferring genesinto plants. Biol Sci. 1985;221:1229-31.

  17. Sudhir P, Murthy SDS. Effects of saltstress on basic processes of photosynthesis.Photosynthetica. 2004;42(4):481-6.

  18. Clough SJ, Bent AF. Floral dip: a simplifiedmethod for Agrobacterium-mediatedtransformation in Arabidopsis thaliana.Plant J. 1998;16(6):735-43.

  19. Tamura K, Peterson D, Peterson N,Stecher G, Nei M, Kumar S. MEGA5:Molecular evolutionary genetics analysisusing maximum likelihood, evolutionarydistance, and maximum parsimony methods.Mol Biol Evol. 2011;28(10):2731-9.

  20. Banks JA, Nishiyama T, Hasebe M,Bowman JL, Gribskov M, dePamphilisC, et al. The Selaginella genome identifiesgenetic changes associated with theevolution of vascular plants. Science.2011;332(6032):960-3.

  21. Soderlund C, Descour A, KudrnaD, Bomhoff M, Boyd L, Currie J, et al.Sequencing, mapping, and analysis of27,455 maizes full-length cDNAs. PLOSGenetics. 2009;5(11):e1000740.

  22. Schnable PS, Ware D, Furton RS, SteinJC, Wei F, Pasternak S, et al. The B73 maizegenome: complexity, diversity, and dynamics.Science. 2009;326(5956):1112-5.

  23. Kang K, Lee K, Park S, Lee S, Kim YS,Back K. Overexpression of rice ferrochelataseI and II leads to increased susceptibility tooxyfluorfen herbicide in transgenic rice. JPlant Biol. 2010;53:291-6.

  24. Suzuki T, Masuda T, Singh DP, Tan F-C,Tsuchiya T, Shimada H, et al. Two types offerrochelatase in photosynthetic and nonphotosynthetictissues of cucumber. Theirdifference in phylogeny, gene expression,and localization. J Biol Chem. 2002;227:4731-7.

  25. Chow KS, Singh DP, Walker AR, Smith AG.Two different genes encode ferrochelatase inArabidopsis: mapping, expression and subcellulartargeting of the precursor protein. PlantJ. 1998;15:531-41.

  26. Alawady A, Alawady A, Ortega P, GrimmB. Nicotiana tabacum ferrochelatase isoformI mRNA, complete cds; plastid [GenBank AccessionNumber JF428143.1]. 2016.

  27. Ortega-Rodes P, Grimm B, Ortega E. Evolutionary,physiological and biotechnologicalaspects of ferrochelatase and heme in higherplants. Biotecnol Apl. 2014;31(3):176-186.

  28. Hey D, Ortega-Rodés P, Fan T, Schnurrer F,Brings L, Hedtke B, et al. Transgenic tobaccolines expressing sense or antisense FERROCHELATASE1 RNA show modified Ferrochelataseactivity in roots and provide experimentalevidence for dual localization of Ferrochelatase1. Plant Cell Physiol. 2016;57(12):2576-85.

  29. Ortega-Rodés P. Ferrochelatase 1 andheme in Nicotiana tabacum: their relatioshipwith stress physiology [PhD thesis].La Habana: Universidad de la Habana;2014.

  30. Papenbrock J, Grimm B. Regulatorynetwork of tetrapyrrole biosynthesis of intracellularsignaling involved in metabolic anddevelopmental control of plastids. Planta.2001; 213:667-81.

  31. Porra RJ, Lascelles J. Studies of ferrochelatase.The enzymatic formation ofhaem in proplastids, chloroplasts and plantmitochondria. Biochem J. 1968;108:343.

  32. Smith AG, Santana MA, Wallace-CookADM, Roper JM, Labbe-Bois R. Isolation ofa cDNA encoding chloroplast ferrochelatasefrom Arabidopsis thaliana by functionalcomplementation of a yeast mutant. J BiolChem. 1994;269:13405-13.

  33. Chow K, Singh DP, Roper JM, SmithAG. A single precursor protein for Ferrochelatase-I from Arabidopsis is importedin vitro into both chloroplasts and mitochondria.J Biol Chem. 1997;272(44):27565-71.

  34. Fan T, Roling L, Meiers A, Brings L, Ortega-Rodés P, Hedtke B, et al. Complementationstudies of the Arabidopsis fc1 mutant substantiateessential functions of ferrochelatase1 during embryogenesis and salt stress. PlantCell Environ. 2019;42(2):618-632.

  35. Borisjuk L, Rolletschek H. The oxygenstatus of the developing seed. New Phytol.2009;182:17-30.




2020     |     www.medigraphic.com