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2021, Number 2

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Biotecnol Apl 2021; 38 (2)

Phenotypic and genetic stability of tobacco plants derived from cryopreserved seeds

Pérez-Rodríguez JL, Lorente GY, Martínez-Montero ME, González JL, Rodríguez-Alfonso D

Language: English
References: 49
Page: 2212-2215
PDF size: 792.42 Kb.


ABSTRACT

The examination of genetic stability is one of the most important issue in the evaluation of cryopreservation techniques. This research was aimed at determining if there are changes at the phenotypic and molecular level in tobacco plants derived from cryopreserved seeds. The seeds of the cultivar “Sancti Spíritus 96” were harvested 35 d after anthesis, dried to a water content of 2.1 % and kept for one year in liquid nitrogen (–196 ºC) or at 5 ºC. In the seedbed phase, the morphological characterization was carried out at 20, 30 and 40 d after sowing and three growth indices were calculated from the dynamics between 20 and 40 d. In the field phase, the morphological characterization was carried out 65 d after transplantation. The tobacco plants derived from each treatment did not show morphological differences, both in seedbed phase and in field phase. In addition, they did not show significant differences in the growth rates determined in the seedling phase. The genetic stability of the seedlings was evaluated by means of RAPD markers. No polymorphism was detected between the DNA from the plants derived from each treatment. In this way, both the base and duplicate collections can be conserved in liquid nitrogen, with which the tobacco germplasm will be available for long-term use by plant breeders in obtaining new cultivars.


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