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Revista Latinoamericana de Microbiología

2000, Number 3

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Microbiología 2000; 42 (3)

Variation of peripheral blood BoCD2 + , BoCD4+ , and BoCD8 + lymphocytes, the BoCD4:BoCD8 index and IgG antibodies in bovines infected and rechallenged with isolates of Anaplasma marginale of Mexican Origin.

Bautista GCR, Angeles L, García OMA, García TD, Soto C, Monaño ELF

Language: English
References: 43
Page: 101-109
PDF size: 146.79 Kb.


ABSTRACT

Peripheral blood (PB) BoCD2 + , BoCD4 + , and BoCD8 + lymphocyte levels, the BoCD4:BoCD8 index (CDIndex) and levels of IgG antibodies to Anaplasma marginale were assessed by flow cytofluorometry and ELISA, respectively, in four bovines during infection and reinfection with mexican A. marginale isolates; and in four other animals which served as noninfected controls. During primary infection, the CDIndex and antibody levels increased after the rickettsemia dropped and there was a strong correlation between the CDIndex and the IgG titres. Upon rechallenge, the CD Index and IgG titres did not change significantly with respect to the values observed at the beginning of the study. Additionally, PB lymphocyte levels were estimated in 12 normal intact bovines. The results indicate that BoCD4+ lymphocytes help in the control of infection with mexican Anaplasma isolates in the primary infection and that the IgG antibodies play a minor role in protection from infection.


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