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Revista Latinoamericana de Microbiología

2000, Número 3

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Microbiología 2000; 42 (3)


Efecto de las mutaciones en punto λ tI sobre la terminación de la transcripción mediada por la RNA polimerasa T3.

Cisneros B, Bermúdez CRM, Sánchez A, Montañez C

Idioma: Español
Referencias bibliográficas: 32
Paginas: 95-100
Archivo PDF: 87.62 Kb.


RESUMEN

El terminador de la transcripción tI, que esta localizado aproximadamente a 280 nucleótidos del gen int del bacteriófago λ, es considerado un terminador intrínseco, ya que presenta una estructura secundaria rica en G-C seguida de una serie de 6 residuos de uracilo. Con la finalidad de estudiar la participación de la secuencia de tI en la terminación de la transcripción mediada por la RNA polimerasa del bacteriófago T3, en este estudio se analizó el comportamiento in vitro de tres diferentes mutaciones puntuales (tI1, tI2 y tI3) que se localizan en la región de tallo-burbuja de este terminador. Para llevar a cabo los estudios de transcripción in vitro, los fragmentos de DNA que contenían a las mutaciones mencionadas se clonaron en el vector pBS+ que porta al promotor del bacteriófago T3. Las mutaciones tI causaron una disminución en la eficiencia de la terminación de la transcripción, de un 85% que se obtuvo con el terminador silvestre, a un 46-60%.


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