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Revista Latinoamericana de Microbiología

2000, Number 2

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Microbiología 2000; 42 (2)

Evaluation of the double agar gel immunodiffusion test with serum agglutination in plate, tube, and 2-mercaptoethanol for cows vaccinated with five different reduced-dosages of B. abortus-Strain-19.

Modolo JR, de Magalhaes-Lopes CA, Bacila AJ, Pizarro-Borges CH, Henri-Dierckx DP, Almeida-Martins L

Language: English
References: 14
Page: 69-72
PDF size: 83.28 Kb.


ABSTRACT

The control of bovine brucellosis requires the differential serological diagnosis of the nonvaccinated animals and animals vaccinated with B. abortus-S-19. This is almost impossible using the classic serological diagnostic tests, such as seroagglutination in plate (SAP), seroagglutination in tube (SAT), and 2-mercaptoethanol (2Me). In this study, serological titers, induced by 5 reduced-dosages, up to three times, corresponding to 6 x 105, 106, 107, 108, and 109 CFU/ml of S-19 were seen during a 120-day period and analyzed in 5 groups of cows numbering 7, 6, 13, 12, and 11 cows, respectively. The titers showed variations up to 400 IU using SAP, up to 800 IU using SAT and 2Me. No precipitation of antigen poly-O of B. abortus 1119-3 was observed using double agar gel immunodiffusion (AGID). Considering the need for further investigation, this allows us to consider AGID a useful diagnostic test to distinguish between naturally infected seropositive animals and vaccinated cows with different reduced-dosages of B. abortus-S-19.


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