Mendoza-Hernández C, Medina-de la Cruz O, Díaz-Palma RM, Carranza-Betancourt O, Gallegos-García M, Escoto-Chávez SE, Gallegos-García V

Language: Spanish
References: 24
Page: 26-36
PDF size: 430.77 Kb.

ABSTRACT

Introduction. Essential oils have a large number of compounds that have a fungicidal effect and in recent years their use has been considered as a possible treatment that allows infection control. Candida albicans is a part of the microbiota and is a pathogenic yeast of medical importance that in certain pathologies, such as cervical intraepithelial neoplasia (CIN), various cancers, and patients with human immunodeficiency syndrome, can cause superficial or systemic infections. Objective. To evaluate the fungicidal effect of Chrysactinia mexicana A. Gray on clinical isolates of C. albicans from female patients in San Luis Potosi with different degrees of CIN. Material and Methods. The essential oil of C. Mexicana was extracted by steam entrainment. The 96-well plate microdilution tech-nique was used to determine the fungistatic and fungicidal concentration of the essential oil and linalyl acetate. 24 h, stationary and logarithmic growth phase tests were performed on 11 clinical isolates of C. albicans from patients with CIN and ATCC strain. Subse-quently, growth was observed in petri dishes with YPD agar for 48h. Results. A fungicidal effect was observed in C. albicans strains Ca7 and SC5314 with 3.71mg/ml and 4.64mg/ml respectively C. albicans Ca7 showing 100% inhibition of the germ tuve at the con-centration of 0.37mg/ml of the essential oil of C. mexicana. Conclusions. The essential oil of C. mexicana has a fungicidal and fungistatic effect in clinical isolates from patients with CIN.

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