Palacios-Arriaga JM, Gutierrez-Pabello JA, Chavez-Gris G, Hernandez-Castro R

Language: English
References: 31
Page: 27-34
PDF size: 220.03 Kb.

ABSTRACT

The efficacy of a florfenicol premix was studied in weaning pigs experimentally inoculated with Actinobacillus pleuropneumoniae. Twenty five clinically healthy pigs were distributed into 3 groups; group A non-medicated, groups B and C orally medicated with 20 and 40 ppm of florfenicol respectively. The pigs were fed during 12 consecutive days and on day 5 all the groups were challenged with A. pleuropneumoniae serotype 1. All the animals in Group A developed clinical signs. Most of the pigs in the medicated groups maintained a good health status. Postmorten examination revealed severe pleuropneumonia in pigs from the control group and pneumonic lesions in 40% of the animals treated with 20 ppm of florfenicol. Development of pleuropneumonia was prevented in all the pigs medicated with 40 ppm of florfenicol. Actinobacillus pleuropneumoniae was recovered from the lungs of all control animals and from one pig of each of the medicated groups, however, the avidin biotin peroxidase (ABC-P) method detected the presence of the microorganism in all the animals. We demonstrated that medication with feed containing 40 ppm of florfenicol blocked efficiently the signs and lesions caused by A. pleuropneumoniae and increased the daily body weight gain.

REFERENCES

1. Adams, P. E., K. J., Varma, T. E. Powers and J. F. Lamendola. 1987. Tissue concentrations and pharmacokinetics of florfenicol in male veal calves given repeated doses. Am. J. Vet. Res. 48:1725-1732.

2. Biberstein, E. L., A. Gunnarson and B. Harvell. 1977. Cultural and biochemical criteria for the identification of Haemophilus spp. from swine. Am. J. Vet. Res. 38:7-11.

3. Bretzlaff, K. N., C. A. Neff-Davis, R. S. Ott, G. D. Koritz, B. K. Gustaffsson and L. E. Davis. 1987. Flo rfenicol in non-lactating dairy cows: pharmacokinetics, binding to plasma proteins, and effects on phagocytosis by blood neutrophils. J. Vet. Pharmacol. Therap. 10:233-240.

4. Cannon, M., S. Harford and J. Davies. 1990. A comparative study on the inhibitory actions of chlora mphenicol, thiamphenicol and some fluorinated analogs. J. Antimicrob. Chemother. 26:307-317.

5. Fales, W. H., L. G. Morehouse, K. R. Mittal, C. Bean-Knudsen, S. L. Nelson, L. D. Kintner, J. R. Turk, M. A. Turk, T. P. Brown. and D. P. Shaw. 1989. Antimicrobial susceptibility and serotypes of Actinobacillus (Haemophilus) pleuropneumoniae recovered from Missouri swine. J. Vet. Diagn. Invest. 1:16-19.

6. Fenwick, B. and S. Henry. 1994. Porcine pleuropneumonia. J. Am. Vet. Med. Assoc. 204:1334-1340.

7. Gutierrez Martin C. B., R. de la Fuente Lopez, R. I. Tascon Cabrero, F. J. Garcia Pena, J. A. Vazquez Boland and E. F. Rodriguez Ferri. 1991. Diagnóstico de la pleuroneumonia porcina. Med. Vet. 8:321-335.

8. Gutierrez, C. B., S. Piriz, S. Vadillo and E. F. Rodriguez. 1993. In vitro susceptibility of Actinobacillus pleuropneumoniae strains to 42 antimicrobial agents. Am. J. Vet. Res. 54:546-550.

9. Gutierrez, C. B., J. I. Rodriguez-Barbosa, J. Suarez, R. I. Tascon and E. F. Rodriguez-Ferri. 1993. Evaluation of an immunoperoxidase technique using an only biotin- labeled antibody for the demonstration of Actinobacillus pleuropneumoniae in tissue sections. J. Vet. Med. 40:81-88.

10. Hunneman, W. A., A. Pijpers, J. Lommerse, A. P. P. Crauwells and J. H. M. Verheijden. 1994. Prophylaxis of pleuropneumonia in pigs by in-feed medication with oxytetracycline and the subsequent transmission of infection. Vet. Rec. 134:215-218.

11. Ibargoyen, G. S., C. J. Perfumo, A. R. Massone, A. A. Martin and E. J. Jimeno. 1989. The use of immunoperoxidase techniques for the identification of Actinobacillus pleuropneumoniae in tissue sections. Isr. J. Vet. Med. 45:18-23.

12. Inamoto, T., K. Kikuchi, H. Iijima, Y. Kawashima, Y. Nakai and K. Ogimoto. 1994. Antibacterial activity of tilmicosin against Pasteurella multocida and Actinobacillus pleuropneumoniae isolated from pneumonic lesions in swine. J. Vet. Med. Sci. 56, 917-921.

13. Lobell, R. D., K. J. Varma, J. C. Johnson, R. A. Sams, D. F. Gerken and S. M. Ashcraft. 1994. Pharmacokinetics of florfenicol following intravenous and intramu scular doses to cattle. J. Vet. Pharmacol. Therap. 17:253-258.

14. Mengelers, M. J. B., B. van Klingeren and A. S. J. P. A. M. van Miert. 1990. In vitro susceptibility of some porcine respiratory tract pathogens to aditoprim, trimetoprim, sulfadimethoxine, sulfamethoxazole and combinations of these agents. Am. J. Vet. Res. 51:1860-1863.

15. Mengelers, M. J. B., H. A. Kuiper, A. Pijpers, J. H. M. Verheijden and A. S. J. P. A. M. van Miert. 1991. Prophylactic feed medication with sulfonamidetrimethoprim combination and residue depletion in pigs. Acta Vet. Scand. Supplement 87:363-365.

16. Paape, M. J., R. H. Miller and G. Ziv. 1990. Effects of florfenicol, chloranphenicol and thiamphenicol of phagocytosis, chemiluminescence, and morphology of bovine polymorphonuclear neutrophil leucocytes. J. Dairy Sci. 73:1734-1744.

17. Pijpers, A., E. J. Schoevers, J. C. Vernooy, L. A. van Leengoed and J. H. Verheijden. 1991. The prophylactic effect of doxycycline in-feed medication against an Actinobacillus pleuropneumoniae challenge in pigs. Acta Vet. Scand.- Supplement, 87:369-371.

18. Raemdonck, D. L., A. C. Tanner, S. T. Tolling and S. L. Micener. 1994. Antimicrobial susceptibility of Actinobacillus pleuropneumoniae, Pasteurella multocida and Salmonella cholerasuis isolates from pigs. Vet. Rec. 137:5-7.

19. SAS Institute, Inc. 1989. SAS Language and Procedures: Usage. Version 6, St. Edn. Cary, North Carolina, USA.

20. Schwarz, S. 1994. Emerging chloramphenicol resistance in Staphyloccoccus lentus from mink following chloramphenicol treatment: characterisation of the resistance gene. Vet. Microbiol. 41:51-61.

21. Sebunya, T. N. K. and J. R. Saunders. 1983. Haemophilus pleuropneumoniae infections in swine: A review. J. Am. Vet. Med. Ass. 182:1331-1337.

22. Shaw, W. V. 1984. Bacterial resistance to chlora mphenicol. Br. Med. Bull. 40:36-41.

23. Smith, I. M., A. Mackie and J. Lida. 1991. Effect of giving enrofloxacin in the diet to pigs experimentally infected with Actinobacillus pleuropneumoniae. Vet. Rec. 129:25-29.

24. Stephano-Hornedo, A., C. Diaz-Rayo and F. Vazquez-Rojas. 1988. Evaluación de un nuevo derivado del acido quinolin carboxilico (enrofloxacina) en el tratamiento de la infección experimental por Haemophilus pleuropneumoniae en cerdos. Estudio preliminar. Vet. Mex. 19:85-90.

25. Syriopoulou, V. P., A. L. Harding, D. A. Goldman and A. L. Smith. 1981. In vitro antibacterial activity of fluorinated analogs of chloramphenicol and thiamphenicol. Antimicrob. Agents. Chemother. 19:294-297.

26. Varma, K. J., P. E. Adams, T. E. Powers, J. D. Powers and J. F. Lamendola. 1986. Parmacokinetics of florfenicol in veal calves. J. Vet. Pharmacol. Therap. 9: 412-425.

27. Vassort-Bruneau, C., M. C. Leage-Descauses, J. L. Martel, J. P. Lafont and E. Chaslus-Dancla. 1996. CAT III chloramphenicol resistance in Pasteurella haemolytica and Pasteurella multocida isolated from calves. J. Antimicrob. Chemother. 38:205-213.

28. Villadolmat, G. C., O. T. Angeles, V. L. Sagahon, J. C. Machin, J. A. de la C. Jacobs, M. De la G. Amaya and A. C. Carrasco. 1988. Resistencia antimicrobiana no codificada por plásmidos en Actinobacillus pleuropneumoniae serotipo 1. Vet. Mex. 19:315-320.

29. Willson, P. J., G. Falk and S. Klashinsky. 1987. Detection of Actinobacillus pleuropneumoniae infection in pigs. Can. Vet. J. 28:111-116.

30. Willson, P. J. 1990. Haemophilus, Actinobacillus, Pasteurella: Mechanisms of resistance and antibiotic therapy. Can. J. Vet. Res. 54:S73-S77.

31. Yung-Fu, C., S. Jiarong, J. S. Sang and H. L. Donald. 1992. Sequence analysis of the ROB-1 b-lactamase gene from Actinobacillus pleuropneumoniae. Vet. Microbiol. 32:319-325.