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2020, Number 4

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Biotecnol Apl 2020; 37 (4)

Evaluation of plantain and cassava peels wastes for amylases production by Bacillus amyloliquefaciens A16

Heredia MJP, Sánchez CEM

Language: Spanish
References: 29
Page: 3201-3205
PDF size: 309.39 Kb.


ABSTRACT

The substrates used to produce microbial amylases are one of the principal factors affecting the process yield because the enzyme activity and production costs depend on their characteristics. Most industrial amylases produced are obtained from synthetic substrates formulated from expensive commercial starches. It is important to search for new affordable substrates to obtain microbial amylases, with specific properties that allow their application in several industrial processes. The agro-industrial wastes with starch in their composition may be used as affordable substrates for amylase production. In this study, the enzymatic activities produced from Bacillus amyloliquefaciens (Isolated A16) in culture media made from plantain and cassava peels, with a starch concentration of 7.74 and 5.27 % w/w, were compared to the enzyme activity obtained in a standard media made from soluble starch. During fermentation, samples were taken every 12 h to obtain enzymatic extracts in which the starch degradation was qualitatively evaluated, and the amylase activity was determined at pH 6.5. After 84 h of fermentation, the plantain peels media presented a major amylase activity (96.52 ± 0.36 U/mL) at pH 7.0, results higher to those obtained in the starch standard media (74.01 U/mL) and cassava peels media (40.94 ± 0.19 U/mL). Hence, this showed that plantain peels are a usable and accessible substrate that may provide good yields in amylase production.


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