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ISSN 1027-2852 (Electronic)
ISSN 0864-4551 (Print)

2020, Number 3

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Biotecnol Apl 2020; 37 (3)

New knowledge for increased efficiency of Araceae micropropagation in vitro

Galvez GD, Rodríguez MS, Beovides GY, Milián JMD, Gómez KR, Posada PL

Language: English
References: 13
Page: 3501-3503
PDF size: 271.07 Kb.


ABSTRACT

In vitro culture is a reliable method for plant multiplication free of microbial contaminants. In this work, a series of research were done to increase the number of explants of Araceae cultivars aimed for propagation in biofactories. Prior treatment of corms with 3.0 % sodium hypochloride for 20-25 min favored the in vitro establishment of 85.38 % of apexes. The best choice conditions were MS culture medium with salts and vitamins, further supplemented with 0.5 mg/L 6-BAP, during the establishment phase, and the same medium but with 4.0 mg/L 6-BAP during multiplication. During the establishment phase, undecapitated corms were the main source for explants, obtaining more than 300 meristems per corm. Microbial contamination was reduced from 40 to 2 %, growth of explants increased and also survival index. The best performance was attained during multiplication by applying three subcultures in semisolid medium, combined with temporary or constant immersion semiatutomated systems with culture medium aeration. The multiplication coefficient was further incremented by by applying differen cutting to explants than the traditional one (50 % decapitation of the shoot and bevel cut. A manual was elaborated for the management of plantlets at the acclimatization phase, with the inclusion of several modifications and new contributions to pre-existing methodologies, which further provides novelty and a high scientific impact for taro in vitro culture. This work granted the Annual Award of the National Academy of Sciences of Cuba for the year 2019.


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