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2020, Number 1

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Rev Cubana Plant Med 2020; 25 (1)

Antibacterial and modulatory potential of antibiotics from leaves of Allamanda puberula A.DC. (quatro-patacas)

Leite NAP, da Silva BÍM, Figueiredo LSN, Oliveira TLE, Torres SC, de Souza JDL, Gomes FF

Language: Portugués
References: 39
Page: 1-14
PDF size: 507.32 Kb.


ABSTRACT

Introduction: Some plant extracts have been tested for new compounds with recognized antibacterial activity.
Objectives: Explore the ethanolic extract of A. puberula leaves for the presence of chemical compounds, antibacterial activity and as modifier of resistance to antibiotics in classes of aminoglycosides and lincosamides.
Methods: The ethanolic extract was obtained from fresh leaves of Allamanda puberula and subjected to phytochemical analysis by the colorimetric method. In vitro antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli pattern strains was evaluated by broth microdilution to determine the minimum inhibitory concentration. Modulation of antibiotic activity was tested at a subinhibitory concentration.
Results: The natural product analyzed was found to contain secondary metabolites of recognized biological activity. However, clinically relevant antibacterial activity of the isolated extract against the lineages studied was not observed. The response was found to improve when the natural product was associated to aminoglycosides. The activity of the amikacin concomitant to the extract against S. aureus and E. Coli causes a significant minimum inhibitory concentration reduction of 182.29 μg/ml to 32.55 μg/ml and 42.315 μg/ml to 26.04 μg/ml, respectively. Association of gentamicin to the extract against P. aeruginosa caused a minimum inhibitory concentration reduction of 104.17 μg/ml to 15.46 μg/ml.
Conclusions: Association of the antibiotic to the natural product reduced the therapeutic dose. This activity may be due to the presence of secondary metabolites in the extract with recognized antibacterial activity.


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