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2019, Number 4

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Rev Cubana Plant Med 2019; 24 (4)

Use of infusions from medicinal plants to control Candida albicans on toothbrushes

Cota JT, Bonfim SGA, Kozusny-Andreani DI, Rodrigues FDF

Language: Portugués
References: 30
Page: 1-15
PDF size: 341.09 Kb.


ABSTRACT

Introduction: Toothbrushes have a great load of microorganisms, for they mainly become contaminated through contact with the oral cavity.
Objective: Evaluate the antifungal activity of infusions from medicinal plants to control Candida albicans on toothbrushes.
Methods: The medicinal plants used were Camellia sinensis (L.) Kuntze (white tea, black tea, green tea), Matricaria chamomilla L. (camomile), Pimpinella anisum L. (anise), Cinnamomum zeylanicum L. (Ceylon cinnamon), Caryophyllus aromaticus L. (clove), Mentha piperita L. (peppermint) and Eugenia uniflora L. (pitanga). The infusions were prepared with 100 ml distilled water subjected to a boiling process and 1 g dehydrated leaves allowed to remain suspended in the liquid for 5 minutes. The toothbrushes were soaked for 15 min into fungal solution containing 1.2 x 106 CFU ml-1. Next, they were soaked into the different treatments for 15, 30, 60, 120, 240 and 480 min. The antiseptic chlorhexidine was used as control. They were put into the NaCl (5%) solution and stirred; 0.1 ml of the solution obtained was used for inoculation of Sabouraud agar plates incubated at 37ºC for 24 hours.
Results: All the treatments were effective after 480 minutes of contact with the toothbrushes, but peppermint and pitanga displayed the same efficacy as chlorhexidine at 30 min.
Conclusions: The infusions tested displayed efficacy in the disinfection of toothbrushes contaminated with Candida albicans, which allows us to state that medicinal plants are a quite viable alternative for antifungal disinfection, as long as they are used at the appropriate concentration and time.


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