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2019, Number 2

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Rev Cubana Plant Med 2019; 24 (2)

Sample size for Rosmarinus officinalis L. (Rosemary) plants grown on various substrates

Schoffel A, Koefender J, Nicolodi CJ, Pascoal GD, Cattaneo HR

Language: Portugués
References: 23
Page: 1-16
PDF size: 401.73 Kb.


ABSTRACT

Introduction: The use of medicinal plants to treat a variety of diseases is on the increase. An example is Rosmarinus officinalis L., one of the species most commonly used for phytotherapeutic purposes.
Objective: Evaluate the propagation by cuttings of R. officinalis and determine the sample size in terms of number of plants, to examine the average characteristics measured in the various substrates.
Methods: The following six substrates were used: commercial (Mec Plant®) (100 %), commercial (50 %) + soil (50 %), organic (100 %), commercial (75 %) + organic (25 %), commercial (50 %) + organic (50 %) and soil (100 %). A total 128 cuttings were planted on each substrate on polystyrene trays and were kept in a greenhouse for 60 days. After planting, sampling was conducted with 60 plants, bearing in mind the fresh and dry weight of the aerial parts and the roots, and the length of the largest root. Estimation was made of measures of central tendency and their variability. Sample size was determined by a second sampling process in which 2 000 cuttings were included with their corresponding replacements. Such determination was based on the total number of plants, trying to maintain the amplitude of the 95 % confidence interval at values equal to 10 %, 20 %, 30 % and 40 % of the average estimated.
Results: Commercial substrate (100 %) and the combinations commercial substrate (50 %) + organic substrate (50 %) and commercial substrate (75 %) + organic substrate (25 %), were the most outstanding. Sample size for the characteristics evaluated varied between the substrates.
Conclusions: Commercial substrate, whether alone or mixed with organic substrate, is more favorable to plant R. officinalis cuttings. Evaluation of 42 plants is sufficient to obtain the average value for the features studied, as long as the amplitude of the confidence interval is 40 %.


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