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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2021, Number 3

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Rev Biomed 2021; 32 (3)

Resiniferatoxin treatment exhibits anti-inflammatory properties in a murine model of lipopolysaccharide-induced inflammation

Muñoz-Carrillo JL, Villalobos-Gutiérrez PT, Puebla-Pérez AM, López-Luna A, Flores-de la Torre JA, Alvarez-Barreto I, Gutiérrez-Coronado O

Language: Spanish
References: 40
Page: 137-146
PDF size: 1177.46 Kb.


ABSTRACT

Introduction. Inflammation is a protective physiological response of the immune system against various stimuli, such as infection or tissue damage, which, by not being resolved properly, can be harmful to the host. In the search for new therapeutic alternatives that inhibit the inflammatory response, several studies have reported the use of various molecules, such as resiniferatoxin (RTX), a potent agonist of the transient receptor potential vanilloid type 1 (TRPV1).
Objective. To evaluate whether RTX treatment exhibits antiinflammatory properties, using a murine model of lipopolysaccharide (LPS)-induced inflammation.
Material and methods. BALB/c mice were stimulated with LPS and subsequently treated with dexamethasone (DEX), capsaicin (CAP), Bay 11-7082, capsazepin (CPZ) and RTX. In addition, other groups of mice were c stimulated with LPS and then treated with Bay 11-7082 and CPZ plus RTX. After treatment, plasma levels of prostaglandin (PG)-E2, nitric oxide (NO), interleukin (IL)-1β and tumor necrosis factor (TNF)- α were quantitatively determined using Enzyme-Linked ImmunoSorbent Assay (ELISA) kits.
Results. Treatment with RTX significantly decreased plasma levels of PGE2, NO, IL-1β and TNF- α. Likewise, it was observed that the treatments with Bay 11-7082 and CPZ plus RTX showed a synergistic anti-inflammatory effect, observing a more pronounced significant decrease in plasma levels of PGE2 and TNF- α.
Conclusion. These findings suggest that treatment with RTX shows anti-inflammatory properties, apparently associated with the nuclear factor (NF)- κB signaling pathway, independent of the TRPV1 receptors, placing RTX as a potential drug in the treatment of inflammatory diseases.


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