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2002, Number 3

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Bioquimia 2002; 27 (3)

Antimicrobial susceptibility and plasmid profile in Neisseria gonorrhoeae strains isolated in Cuba

Llorente CA, Sosa J, Llanes R, Pérez J, Hernandez J

Language: Spanish
References: 35
Page:
PDF size: 207.54 Kb.


ABSTRACT

Thirty-seven strains of Neisseria gonorrhoeae isolated from several province of Cuba were studied; the plasmid profile was analysed in 37 isolates and minimal inhibitory concentration (MIC) was determined in 34 strains for several antimicrobial drugs (penicillin, tetracycline, spectinomycin, cefuroxime, ciprofloxacin, cefotaxime and ceftriaxone). The 65.63% were resistant to the penicillin (MIC ≥ 2µg/mL) and 59.37% to the tetracycline (MIC ≥ 2µg/mL). All the strains were sensitive to cephalosporins studied, spectinomycin and ciprofloxacin. Of the 32 strains 19 were PPNG (59.37%), 3 TRNG (9.38%) and only 1 showed chromosomal resistance to tetracycline. The plasmids of 3.2 Mda and 4.4 Mda were isolated from 15 and 4 strains respectively. In 3 strains was found the 25.2 Mda plasmid. The 24.5 Mda plasmid was significantly associated with the 3.2 Mda plasmid (p= 6,412 x 10 e-3), being predominant the 2.6/3.2/24.5 Mda plasmid profile (13 strains). In six strains (16.21%) was not possible find plasmids. A high incidence of strains with high level of resistance to penicillin was demonstrated. The determination of the plasmid profile constituted an useful tool in the characterization of strains of Neisseria gonorrhoeae, contributing to knowledge of the phenomenon of the antimicrobial resistance of strains circulating in Cuba.


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