medigraphic.com

2002, Número 3

<< Anterior Siguiente >>

Bioquimia 2002; 27 (3)


Bacteriología
Susceptibilidad antimicrobiana y perfil plasmídico en cepas de Neisseria gonorrhoeae aisladas en Cuba

Llorente CA, Sosa J, Llanes R, Pérez J, Hernandez J

Idioma: Español
Referencias bibliográficas: 35
Paginas:
Archivo PDF: 207.54 Kb.


RESUMEN

Se estudiaron 37 cepas de Neisseria gonorrhoeae aisladas en diferentes provincias de Cuba; se analizó el perfil plasmídico en 37 aislamientos y en 34 se determinó la concentración inhibitoria mínima (CIM) frente a diferentes antimicrobianos (penicilina, tetraciclina, espectinomicina, cefuroxima, ciprofloxacina, cefotaxima y ceftriaxona). El 65.63% de las cepas estudiadas resultaron resistentes a la penicilina (CIM ≥2 µg/mL) y el 59.37% a la tetraciclina (CIM≥2 µg/mL). Todas las cepas fueron sensibles a las cefalosporinas, espectinomicina y la ciprofloxacina. Se encontraron 19 cepas PPNG (59.37%), 3 TRNG (9.38%) y una mostró resistencia cromosomal a la tetraciclina. Se aislaron los plásmidos de 3.2 Mda y 4.4 Mda, en 15 y 4 cepas respectivamente. En 3 cepas encontró el plásmido de 25.2 Mda. El plásmido de 24.5 Mda asoció significativamente con el plásmido de 3.2 Mda (p=6.412 x 10 e-3), siendo el perfil plasmídico predominante el 2.6/3.2/24.5 Mda con 13 cepas. El 16.21 % de las cepas resultó libre de plásmidos. La determinación del perfil plasmídico constituyó una herramienta útil en la caracterización de cepas de Neisseria gonorrhoeae, contribuyendo conocimiento de la resistencia antimicrobiana de las cepas que circulan en Cuba.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. Kampmeier RH. Introduction of sulfonamide therapy for gonorrhea.Sex Trans Dis 1983; 10:81-4.

  2. Wiesner PJ, Holmes KK, Sparling PF. Single doses of methacycline and doxycycline for gonorrhea: a cooperative study of the frecuency and cause of treatment failure. J Infect Dis 1973;127:461-6.

  3. Inga L. Antimicrobial Resistance in Neisseria gonorrhoeae. Clin Infec Dis 1997;24 (Suppl): S93-7.

  4. Falkow S, Elwell LP, de Graof J. A possible model for the developmentof plasmid-mediated penicillin resistance in the gonococcus. In: Catterall RD, Nicols CS. Editors. Sexually Transmitted Diseases: proceedings of a conference sponsored jointly by the Royal Society Medicine and Royal Society Medicine Foundation Inc, I Winpole St. London WIM SALJun 23-25, 1975. London Academic Press 1976:120-3.

  5. Roberts MC. Plasmid of Neisseria gonorrhoeae and Other Neisseria Species.Clin Microbiol Rev 1989; Apr (Suppl):S18-S23.

  6. Knapp J S, Mesola VP, Neal SW, Wi TE, Tuazon C, Manalastas R, et al.Molecular epidemiology, in 1994, of Neisseria gonorrhoeae in Manilaand Cebu City, Republic of the Philippines. Sex Transm Dis 1997;24:1-7.

  7. Pillay M, Chenia NY, Hoosen AA, Pillay B, Pillay D. A novel β-lactamaseplasmid in Neisseria gonorrhoeae. Med Sci Res 1997; 25: 435-6.

  8. Knapp JS, Zenilman JM, Biddle GH, Perkins WE, DeWitt ML, Thomas ML,et al. Frecuency and distribution in the United States of strains of Neisseria gonorrhoeae with plasmid-mediated, high level resistance totetracycline. J Infect Dis 1987; 155: 819-22.

  9. Dillon JR, Yeung K-H. Beta-lactamase plasmids and chromosomally mediated antibiotic resistance in pathogenic Neisseria species. Clin Microbiol Rev 1989; suppl 2: S125-S133.

  10. MINSAP. Serie cronológica. Reporte de casos de Gonorrea. 1999.

  11. Delgado C, Romero F, Saez N, Sotolongo F. Sensibilidad in vitro del gonococo a la penicilina. Rev Cub Hig Epidemiol 1981;27(3):2601-2611.

  12. Llanes R, Sosa J, Martínez I, Llop A, Palma S, Valdés EA, et al.Antimicrobial susceptibility of 42 N. gonorrhoeae strains isolated from1995 in Cuba. Tenth International Pathogenic Neisseria Conference1996Sept.8-13, Baltimore, Maryland, USA, 1996; 51-52.

  13. Sosa J, Llanes R, Rodríguez W, Gutiérrez Y, Guzmán D. Characterizationof Neisseria gonorrhoeae strains isolated from patients withconjunctivitis. Mem Inst Oswaldo Cruz 2000;95: 853-854.

  14. Knapp JS, Rice RJ. Neisseria and Branhamella. In. Murray PR, Baron EJ,Pfaller MA, Tenover FC, Yolken RH. 6th ed. Washington DC: Manual ofClinical Microbiology, American Society for Microbiology; 1995.

  15. National Committee for Clinical Laboratory Standards. Approved stan-dard M100-S9. Performance standard for antimicrobial susceptibilitytesting; Ninth informational supplement; Wayne PA: National Commiteefor Clinical Laboratory Standards. 1999.

  16. Protocols and Applications Guide. Promega Corporation. SecondEdition. WI. USA. 1991.

  17. Chan RK, Thirumoorthy T. A decade of PPNG in Singapore. Ann AcadMed Singapore 1987;16:639-44.

  18. Clendennen TE, Hames CS, Kees ES. In vitro antibiotic susceptibilitiesof Neisseria gonorrhoeae isolates in Phlipines. Antimicrob Chemother1992;36:277-282.

  19. Van Dyck E, Karita E, Abdellati et al. Antimicrobial susceptibilities ofNeisseria gonorrhoeae in Kigali, Rwanda, and trends of resistance between1986 and 2000. Sex Transm Dis 2001; 28(9):539-45.

  20. Finland C, Robertsons H. Susceptibility of strains of Neisseria gonorrhoeaein Malawi. Arch Vener Dis 1992; 35:245-8.

  21. Ison CA. A one-year survey of Neisseria gonorrhoeae isolated frompatients attending an East London genitourinary medicine clinic: antibiotic susceptibility patterns and patients’characteristics.Genitourin Med 1995 Feb;71(1):13-7.

  22. Fiorito S, Galarza P, Pagarno I, et al. Antibiotic resistance ofNeisseria gonorrhoeae in Argentina. Abstract O143 Abstract monograftof the twelth meeting of the international society for STD research,Seville, 1997.

  23. Desai PJ, Morrinson JA, Fleming AF. Penicillinase-producing Neisseriagonorrhoeae in Ndola, Zambia. Transact Royal Soc Trop Med Hyg.1990;84:131.

  24. Chu ML, Ho LJ, Lin HC, Wu YC. Epidemiology of penicillin-resistanceNeisseria in Taiwan, 1960-1990. Clin Infect Dis 1992;14:450-457.

  25. Centers for Disease Control. Plasmid-mediated antimicrobial resistancein Neisseria gonorrhoeae United States, 1988 and 1989. MMWR1990;39:284-7.

  26. Hudson BJ, van der Meijden WI, Lupiwa T, Howard P, Tabua T, TapsallJW, et al. A survey of sexually transmited diseases in five STD clinics inPapua New Guinea. N G Med J 1994;37(3):152-60.

  27. William L, Whittington AB, Knapp JS. Trends in Resistance of Neisseriagonorrhoeae to Antimicrobial Agents in United States. Sex Transm Dis1988;15(4):202-10.

  28. Moodley P, Pillay C, Goga R, Kharsany AB, Sturm AW.Evolution in thetrends of antimicrobial resistance in Neisseria gonorrhoeae isolated inDurban over a 5 year period: impact of the introduction of syndromicmanagement. J Antimicrob Chemother 2001; 48(6):853-9.

  29. Knapp JS, Brathwaite AR, Hinds A, Duncan W,Rice RJ. Plasmid-mediatedantimicrobial resistance in Neisseria gonorrhoeae in Kingston, Jamaica.Sex Transm Dis 1995 May-Jun;22(3):155-9.

  30. Dillon JR, Pauze M. Appearance in Canada of Neisseria gonorrhoeaestrains with a 3.2 megadalton penicillin-producing plasmid and 24.5megadalton transfer plasmid. Lancet 1981;ii:700.

  31. Schipper AM. Further spread of plasmid among different auxotipes ofpenicillinase-producing gonococci. The Lancet 1982;20(2):445.

  32. Centers for Disease Control. Spectinomycin-resistant β-lactamase-producing Neisseria gonorrhoeae-England. MMWR 1982;31:495-6.

  33. Llanes R, Sosa J, Guzmán D, Gutiérrez Y, Llop A, Ricardo O. Neisseriagonorrhoeae resistant to ciprofloxacin. First report in Cuba.Sex TransmDis 2001;28(2):82-83.

  34. Trees DL, Sandul AL, Neal SW, Higa H, Knapp JS. Molecular epidemiologyof Neisseria gonorrhoeae exhibiting decreased susceptibility andresistance to ciprofloxacin in Hawaii, 1991-1999 Sex Transm Dis2001; 28(6):309-14.

  35. Takai K, Koyama Y, Kojima H, Oshi M, Kawab K. Consecutive annual changes in minimum inhibitory concentration in clinically isolatedNeisseria gonorrhoeae strains. Hinyokika Kiyo 1995 Ap;41(4):279-87.




2020     |     www.medigraphic.com