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2019, Número 1

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Biotecnol Apl 2019; 36 (1)


Impacto significativo del funcionamiento del canal de dispersión lateral en los conteos de células T CD4+ con el uso de CD4% easy count kit

Iglesias E, Hernández-Requejo D, Godínez-López MC, Vazquez-Arteaga A

Idioma: Ingles.
Referencias bibliográficas: 24
Paginas: 1301-1306
Archivo PDF: 827.53 Kb.


RESUMEN

El número absoluto y el porcentaje de células T CD4+ son relevantes para el seguimiento de los pacientes infectados con VIH-1. Sin embargo, resultados discordantes entre ambos parámetros determinados mediante citometría de flujo por el CD4% easy count kit (Sysmex-Partec®) indicaron el posible funcionamiento inadecuado del fotomultiplicador, aun cuando los controles de calidad estaban en el rango aceptable. El kit se basa en una plataforma de metodología única de conteo de células volumétrico. Por lo tanto, se analizó dicho funcionamiento como posible fuente de las desviaciones con cantidades fijas de perlas para los canales de dispersión lateral (SSC), fluorescencia (FL-1) y FL-2. Además, se seleccionó a los canales de dispersión frontal (FSC) y FL-3 como controles, y se calculó el coeficiente de variación (CV) y la precisión. Las concentraciones de las perlas (conteos/mL) bajo el pico del histograma de cada canal óptico se compararon mediante análisis de regresión lineal. Estos tres parámetros mostraron un buen ajuste para todos los canales, pero la pendiente del canal SSC mostró diferencias altamente significativas (p < 0.01), lo que sugirió su mal funcionamiento. Se detectó otras desviaciones menores mediante comparación del intercepto del FSC con el FL-1 (p = 0.03) y FL-3 (p = 0.02). Estos resultados explican discordancias anteriores de este kit y muestra una limitación no detectada de los controles de calidad rutinarios. La metodología propuesta proporciona nuevos controles de calidad a incorporar por el usuario, para detectar posibles errores en los fotomultiplicadores en cualquier citómetro de flujo, incluso sin un deterioro significativo en su funcionamiento.


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