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2019, Número 1

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Biotecnol Apl 2019; 36 (1)


Estudio de caracterización epitópica, acoplamiento molecular y simulación dinámica molecular de dos proteínas inmunogénicas principales del virus de la viruela del canario

Mohammadi E, Pirkhezranian Z, Monhemi H, Razmyar J, Tahmoorespur M, Sekhavati MH

Idioma: Ingles.
Referencias bibliográficas: 52
Paginas: 1211-1218
Archivo PDF: 1750.74 Kb.


RESUMEN

El virus de la viruela del canario (CNPV) infecta a estas aves, con alta mortalidad y pérdidas económicas significativas especialmente en los países del Medio Oriente. Existen reportes de importaciones no autorizadas e ilegales de vacunas contra el CNPV propagadas en embriones, liofilizadas y vivas para su uso contra la enfermedad en Irán y en la región. El propósito de este trabajo fue preparar las condiciones para el diseño de una vacuna peptídica contra la enfermedad causada por el CNPV. Se seleccionaron dos proteínas inmunogénicas del CNPV homólogas a los antígenos de Poxine®, HP1-440 y las cepas de virus fowlpox. Se identificó a los péptidos específicos por el MHC II mediante varias herramientas bioinformáticas, los que se modelaron y acoplaron a los receptores HLA-DRB1 0101, 0301, 0401, 0405 y 1501. La estabilidad de los complejos acoplados se evaluó mediante simulaciones de dinámica molecular. También se seleccionó un epitopo experimental del virus Vaccinia para los receptores del MHC I, y su epitopo homólogo en CNPV se acopló a dos receptores BF de pollos. De los 13 epitopos predichos para los receptores del MHC II e identificados mediante acoplamiento y dinámica molecular se escogieron cuatro: IFNAIILWITYAL, LRQLYDVIIPPR, YYNRITSIHM y YRHDDIIAT. El epitopo homólogo al epitopo experimental VP35#1 del virus Vaccinia del canario se seleccionó, dada su respuesta de células T CD8+ de memoria de larga duración, y se propuso el epitopo SLSAYIVSK. Los epitopos candidatos de alta afinidad de unión pudieran incluirse como los más efectivos para diseñar vacunas peptídicas contra la infección por el CNPV.


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