Campuzano MG

Idioma: Español
Referencias bibliográficas: 303
Paginas: 11-68
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RESUMEN

El hemograma es una de las pruebas que más se solicita al laboratorio clínico y, sin duda, una de las que más aporta al clínico en la evaluación del paciente. Desde el punto de vista técnico, se reconocen seis tipos de hemograma y el tipo VI es el que ofrece mayor cantidad de parámetros de utilidad clínica, los cuales se obtienen a partir de una amplia combinación de tecnologías, como el enfoque hidrodinámico y la citometría de flujo. En este módulo se describen los parámetros que componen el hemograma tipo VI, la utilidad clínica, los valores de referencia y algunos aspectos que se deben tener en cuenta al interpretar los resultados y tomar decisiones médicas; además, se hace énfasis en los nuevos parámetros disponibles en el hemograma tipo VI, como el recuento de reticulocitos y los índices derivados, el recuento de granulocitos inmaduros y de plaquetas reticuladas, entre otros, los cuales son parámetros de gran utilidad en diferentes escenarios clínicos y hacen que este hemograma sea el de elección para la práctica médica actual.

REFERENCIAS (EN ESTE ARTÍCULO)

1. Guidelines for the evaluation of blood cell analysers including those used for differential leucocyte and reticulocyte counting and cell marker applications. International Council for Standardization in Haematology: prepared by the ICSH Expert Panel on Cytometry. Clin Lab Haematol 1994; 16: 157-174.

2. Gulati GL, Hyun BH. The automated CBC. A current perspective. Hematol Oncol Clin North Am 1994; 8: 593-603.

3. Perkins SL. Examination of the blood and bone marrow. In: Greer JP, Foerster J, Lukens JN, Rodgers GM, Paraskevas F, Glader B, eds. Wintrobe’s clinical hematology (ed 11th Edition). Philadelphia, PA, USA: Lipincott Williams & Wilkins; 2004: 1-25.

4. Imeri F, Herklotz R, Risch L, Arbetsleitner C, Zerlauth M, Risch GM, et al. Stability of hematological analytes depends on the hematology analyser used: A stability study with Bayer Advia 120, Beckman Coulter LH 750 and Sysmex XE 2100. Clin Chim Acta 2008.

5. Sociedad Colombiana de Patología Clínica. Manual de Codificación, Nomenclatura, Nivelación y Valores de Exámenes de laboratorio Clínico. Medellín, Colombia: Edimeco S.A.; 2007.

6. República de Colombia, Ministerio de Protección Social. Resolución 1896 de 2001 por la cual se sustituye integralmente la Clasificación Única de Procedimientos en Salud.

7. Ruzicka K, Veitl M, Thalhammer-Scherrer R, Schwarzinger I. The new hematology analyzer Sysmex XE- 2100: performance evaluation of a novel white blood cell differential technology. Arch Pathol Lab Med 2001; 125: 391-396.

8. Briggs C, Harrison P, Grant D, Staves J, MacHin SJ. New quantitative parameters on a recently introduced automated blood cell counter--the XE 2100. Clin Lab Haematol 2000; 22: 345-350.

9. Sociedad Colombiana de Patología Clínica. Manual de Codificación, Nomenclatura, Nivelación y Valores de Exámenes de Laboratorio Clínico. Medellín, Colombia: Edimeco S.A.; 2013.

10. Campuzano-Maya G. Del hemograma manual al hemograma de cuarta generación. Medicina & Laboratorio 2007; 133: 511-550.

11. Campuzano-Maya G. Uso y utilidad clínica de la eritrosedimentación. Medicina & Laboratorio 2000; 9: 311-346.

12. Campuzano-Maya G. El hemograma electrónico. Laboratorio Al Día 1995; 5: 28-41.

13. Campuzano-Maya G. El hemograma. Medicina & Laboratorio 1998; 8: 19-32.

14. Campuzano-Maya G. Alteraciones del hemograma relacionadas con los contadores de células. Medicina & Laboratorio 2005; 11: 363-384.

15. Campuzano-Maya G. ¿Cómo obtener un extendido de sangre periférica de óptima calidad? Medicina & Laboratorio 2008; 14: 125-145.

16. Campuzano-Maya G. Utilidad clínica del extendido de sangre periférica: los eritrocitos. Medicina & Laboratorio 2008; 14: 311-357.

17. Campuzano-Maya G. Utilidad clínica del extendido de sangre periférica. Parte 2 de 2: los leucocitos y las plaquetas. Medicina & Laboratorio 2008; 14: 411-xx.

18. Campuzano-Maya G. Utilidad clínica del extendido de sangre periférica: las plaquetas. Medicina & Laboratorio 2008; 14: 511-531.

19. Kim SJ, Kim Y, Shin S, Song J, Choi JR. Comparison study of the rates of manual peripheral blood smear review from 3 automated hematology analyzers, Unicel DxH 800, ADVIA 2120i, and XE 2100, using international consensus group guidelines. Arch Pathol Lab Med 2012; 136: 1408-1413.

20. Pincus MR, Abraham NZ. Interpreting laboratory results. In: McPherson RA, Pincus MR, eds. Henry´s clinical diagnosis and management by laboratory methods (ed 21th edition): Saunders Elsevier; 2007: 76-90.

21. Cartwright GE. Diagnostic Laboratory Hematology (ed 3th ed.). New York, USA, 1963.: Grune and Stratton.

22. Coulter WH. Means for counting particles suspended in a fluid. Vol. U. S. Patent No. 2656508. USA; 1953.

23. Bonner WA, Hulett HR, Sweet RG, Herzenberg LA. Fluorescence activated cell sorting. Rev Sci Instrum 1972; 43: 404-409.

24. Laboratorio Clínico Hematológico. Manual de procedimientos. 2007, Medellín, Colombia.

25. Wintrobe MM. The size and hemoglobin content of the erythrocyte. J Lab Clin Med 1932; 17: 899-911.

26. Bain BJ, Lewis SM, Bates I. Basic haematological techniques. In: Lewis SM, Bain BJ, Bates I, eds. Dacie and Lewis practical haematology (ed 5th edition). Philadelphia, PA, USA: Churchill Livingston; 2006: 26- 57.

27. Zandecki M, Genevieve F, Gerard J, Godon A. Spurious counts and spurious results on haematology analysers: a review. Part II: white blood cells, red blood cells, haemoglobin, red cell indices and reticulocytes. Int J Lab Hematol 2007; 29: 21-41.

28. Zandecki M, Genevieve F, Gerard J, Godon A. Spurious counts and spurious results on haematology analysers: a review. Part I: platelets. Int J Lab Hematol 2007; 29: 4-20.

29. Dickson DN, Wilkinson RL, Noakes TD. Effects of ultra-marathon training and racing on hematologic parameters and serum ferritin levels in well-trained athletes. Int J Sports Med 1982; 3: 111-117.

30. Chatard JC, Mujika I, Guy C, Lacour JR. Anaemia and iron deficiency in athletes. Practical recommendations for treatment. Sports Med 1999; 27: 229-240.

31. Kresie L, Benavides D, Bollinger P, Walters J, Pierson D, Richmond T, et al. Performance evaluation of the application of body fluids on the Sysmex XE-2100 series automated hematology analyzer. Lab Hematol 2005; 11: 24-30.

32. Perkins SL. Examination of the blood and bone marrow. In: Greer JP, Foerster J, Rodgers GM, Paraskevas F, Glader B, Arber DA, et al., eds. Wintrobe’s clinical hematology (ed 12th). Philadelphia, PA, USA: Lipincott Williams & Wilkins; 2009: 1-20.

33. World Health Organization. Recomended method for the determination of packed cell volume by centrifugation (prepared by Expert Pane on cytometry of the International Council for WHO/DIL/00. WHO, Geneva 2000; 1: 1-9.

34. Wintrobe MM. Anemia: Classification and treatment on the basis of differences in the average volume and hemoglobin content of the red corpuscles. Arch Intern Med 1934; 54: 256-261.

35. Tsuda I, Tatsumi N, Zhang Z, Kubota H, Takubo T. Evaluation of Coulter’s cyanide-free reagent for hemoglobinometry. Lab Hematol 1998; 4: 276-280.

36. Nakul-Aquaronne D, Sudaka-Sammarcelli I, Ferrero- Vacher C, Starck B, Bayle J. Evaluation of the Sysmex Xe-2100 hematology analyzer in hospital use. J Clin Lab Anal 2003; 17: 113-123.

37. Beutler E, Waalen J. The definition of anemia: what is the lower limit of normal of the blood hemoglobin concentration? Blood 2006; 107: 1747-1750.

38. WHO. Haemoglobin concentrations for the diagnosis of anaemia and assessment of severity. Vitamin and Mineral Nutrition Information System. Geneva, World Health Organization, 2011 (WHO/NMH/NHD/ MNM/11.1) (http://www.who.int/vmnis/indicators/ haemoglobin. pdf, Consultado en enero 15 de 2013.

39. Nosanchuk JS, Roark MF, Wanser C. Anemia masked by triglyceridemia. Am J Clin Pathol 1974; 62: 838- 839.

40. Shah PC, Patel AR, Rao KR. Letter: Hyperlipemia and spuriously elevated hemoglobin values. Ann Intern Med 1975; 82: 383.

41. Gagne C, Auger PL, Moorjani S, Brun D, Lupien PJ. Effect of hyperchylomicronemia on the measurement of hemoglobin. Am J Clin Pathol 1977; 68: 584-586.

42. Kroll MH, Elin RJ. Interference with clinical laboratory analyses. Clin Chem 1994; 40: 1996-2005.

43. Linz LJ. Elevation of hemoglobin, MCH, and MCHC by paraprotein: how to recognize and correct the interference. Clin Lab Sci 1994; 7: 211-212.

44. Simmons A. Blood storage. Am J Clin Pathol 1982; 77: 116-117.

45. Schoentag RA. Hematology analyzers. Clin Lab Med 1988; 8: 653-673.

46. Bentley SA, Johnson A, Bishop CA. A parallel evaluation of four automated hematology analyzers. Am J Clin Pathol 1993; 100: 626-632.

47. Lampasso JA. Changes in hematologic values induced by storage of ethylenediaminetetraacetate human blood for varying periods of time. Am J Clin Pathol 1968; 49: 443-447.

48. Cornbleet J. Spurious results from automated hematology cell analyzers. Lab Med 1983; 14: 509-514.

49. Bessman JD, Gilmer PR, Jr., Gardner FH. Improved classification of anemias by MCV and RDW. Am J Clin Pathol 1983; 80: 322-326.

50. Hodges RE, Sauberlich HE, Canham JE, Wallace DL, Rucker RB, Mejia LA, et al. Hematopoietic studies in vitamin A deficiency. Am J Clin Nutr 1978; 31: 876- 885.

51. Garcia-Casal MN, Layrisse M, Solano L, Baron MA, Arguello F, Llovera D, et al. Vitamin A and beta-carotene can improve nonheme iron absorption from rice, wheat and corn by humans. J Nutr 1998; 128: 646-650.

52. West MS, Wethers D, Smith J, Steinberg M. Laboratory profile of sickle cell disease: a cross-sectional analysis. The Cooperative Study of Sickle Cell Disease. J Clin Epidemiol 1992; 45: 893-909.

53. Ballas SK, Larner J, Smith ED, Surrey S, Schwartz E, Rappaport EF. The xerocytosis of Hb SC disease. Blood 1987; 69: 124-130.

54. Reed GF, Lynn F, Meade BD. Use of coefficient of variation in assessing variability of quantitative assays. Clin Diagn Lab Immunol 2002; 9: 1235-1239.

55. England JM, Walford DM, Waters DA. Re-assessment of the reliability of the haematocrit. Br J Haematol 1972; 23: 247-256.

56. Holt JT, DeWandler MJ, Arvan DA. Spurious elevation of the electronically determined mean corpuscular volume and hematocrit caused by hyperglycemia. Am J Clin Pathol 1982; 77: 561-567.

57. Strauchen JA, Alston W, Anderson J, Gustafson Z, Fajardo LF. Inaccuracy in automated measurement of hematocrit and corpuscular indices in the presence of severe hyperglycemia. Blood 1981; 57: 1065-1067.

58. Beautyman W, Bills T. Osmotic error in erythrocyte volume determinations. Am J Hematol 1982; 12: 383- 389.

59. Williams LJ. Cell histograms: New trends in data interpretation and classification. J Med Technol 1984; 1: 189.

60. Tur-Kaspa R, Levo Y. Spurious macrocytic anemia. Acta Haematol 1979; 61: 57-58.

61. Bessman JD, Banks D. Spurious macrocytosis, a common clue to erythrocyte cold agglutinins. Am J Clin Pathol 1980; 74: 797-800.

62. Solanki DL, Blackburn BC. Spurious leukocytosis and thrombocytopenia. A dual phenomenon caused by clumping of platelets in vitro. Jama 1983; 250: 2514- 2515.

63. Weiss GB, Bessman JD. Spurious automated red cell values in warm autoimmune hemolytic anemia. Am J Hematol 1984; 17: 433-435.

64. Gleeson SE, McSherry JA. Spurious macrocytosis in infectious mononucleosis. Can Med Assoc J 1983; 129: 1260-1261.

65. Moridaira K, Murakami H, Kitahara T, Tamura J, Matsushima T, Sawamura M, et al. Spurious macrocytosis associated with non-Hodgkin’s lymphoma: report of two cases. Ann Hematol 1993; 66: 55-56.

66. Beautyman W, Bills T. Letter: Osmotic error in measurements of red-cell volume. Lancet 1974; 2: 905- 906.

67. Savage RA, Hoffman GC. Clinical significance of osmotic matrix errors in automated hematology: the frequency of hyperglycemic osmotic matrix errors producing spurious macrocytosis. Am J Clin Pathol 1983; 80: 861-865.

68. Planas AT, Van Voolen GA, Kelly LA. Hyperglycemic macrocytosis in electronically determined mean corpuscular volume. Use of three different automatic cell counters. Ann Clin Lab Sci 1985; 15: 286-291.

69. Beautyman W, Bills T. Osmotic error in red cell measurements of red cell. Lancet 1974; 2: 905-906.

70. Cornbleet J. Spurious results from automated hematology cell counters. Lab Med 1983; 14: 501-514.

71. Imeri F, Herklotz R, Risch L, Arbetsleitner C, Zerlauth M, Risch GM, et al. Stability of hematological analytes depends on the hematology analyser used: a stability study with Bayer Advia 120, Beckman Coulter LH 750 and Sysmex XE 2100. Clin Chim Acta 2008; 397: 68-71.

72. Brittin GM, Brecher G, Johnson CA, Stuart J. Spurious macrocytosis of antibody-coated red cells. Tech Bull Regist Med Technol 1969; 39: 149-153.

73. Patel KJ, Hughes CG, Parapia LA. Pseudoleucocytosis and pseudothrombocytosis due to cryoglobulinaemia. J Clin Pathol 1987; 40: 120-121.

74. Groner W, Simson E. Practical guide to modern hematology analyzers. Chichester, UK: John Wiley and Sons; 1995.

75. Koenn ME, Kirby BA, Cook LL, Hare JL, Hall SH, Barry PM, et al. Comparison of four automated hematology analyzers. Clin Lab Sci 2001; 14: 238-242.

76. Cynober T, Mohandas N, Tchernia G. Red cell abnormalities in hereditary spherocytosis: relevance to diagnosis and understanding of the variable expression of clinical severity. J Lab Clin Med 1996; 128: 259- 269.

77. Michaels LA, Cohen AR, Zhao H, Raphael RI, Manno CS. Screening for hereditary spherocytosis by use of automated erythrocyte indexes. J Pediatr 1997; 130: 957-960.

78. Perkins SL. Pediatric red cell disorders and pure red cell aplasia. Am J Clin Pathol 2004; 122 Suppl: S70-86.

79. D’Onofrio G, Mistretta G, Giordano G, Zini G. Erythropoietic function assessment: Development of methodology-the Sysmex XE-2100TM. Infusion Therapy and Transfusion Medicine 2001; 28: 285-291.

80. Walters JG, Garrity PF. RDW-SD and RDW-CV: Their relationship to RBC distribution curves and anisocitosis. Sysmex J Intern 1993; 3: 40-46.

81. Bain BJ. Blood cells. A practical guide (ed 4th ed). Malden, Massachusetts USA: Blackwell Publishing; 2006.

82. Campuzano-Maya G. Aproximación al diagnóstico etiológico del paciente con anemia. Laboratorio al Día 1995; 5: 195-204.

83. Campuzano-Maya G. Cómo llegar al diagnóstico etiológico del paciente con anemia. Anales de la Academia de Medicina de Medellín 1996; 9: 29-36.

84. Hammersley MW, King RV, Sillivant RE, Liu PI, Teaford MJ, Crook L, et al. High erythrocyte distribution values and possibilities of hemoglobinopathies. Am J Clin Pathol 1981; 75: 370-372.

85. Bessman D, Feinstein D. Anisocytosis shows difference between iron deficiency and thalassemia. Blood 1980; 55: 167.

86. Campuzano-Maya G. Anemia perniciosa. Medicina & Laboratorio 1997; 7: 679-694.

87. Lin CK, Lin JS, Chen SY, Jiang ML, Chiu CF. Comparison of hemoglobin and red blood cell distribution width in the differential diagnosis of microcytic anemia. Arch Pathol Lab Med 1992; 116: 1030-1032.

88. Roberts GT, El Badawi SB. Red blood cell distribution width index in some hematologic diseases. Am J Clin Pathol 1985; 83: 222-226.

89. Tziakas D, Chalikias G, Grapsa A, Gioka T, Tentes I, Konstantinides S. Red blood cell distribution width: a strong prognostic marker in cardiovascular disease: is associated with cholesterol content of erythrocyte membrane. Clin Hemorheol Microcirc 2012; 51: 243- 254.

90. Felker GM, Allen LA, Pocock SJ, Shaw LK, McMurray JJ, Pfeffer MA, et al. Red cell distribution width as a novel prognostic marker in heart failure: data from the CHARM Program and the Duke Databank. J Am Coll Cardiol 2007; 50: 40-47.

91. Tonelli M, Sacks F, Arnold M, Moye L, Davis B, Pfeffer M. Relation between red blood cell distribution width and cardiovascular event rate in people with coronary disease. Circulation 2008; 117: 163-168.

92. Ani C, Ovbiagele B. Elevated red blood cell distribution width predicts mortality in persons with known stroke. J Neurol Sci 2009; 277: 103-108.

93. Hampole CV, Mehrotra AK, Thenappan T, Gomberg-Maitland M, Shah SJ. Usefulness of red cell distribution width as a prognostic marker in pulmonary hypertension. Am J Cardiol 2009; 104: 868-872.

94. Azab B, Torbey E, Hatoum H, Singh J, Khoueiry G, Bachir R, et al. Usefulness of red cell distribution width in predicting all-cause long-term mortality after non-ST-elevation myocardial infarction. Cardiology 2011; 119: 72-80.

95. Ye Z, Smith C, Kullo IJ. Usefulness of red cell distribution width to predict mortality in patients with peripheral artery disease. Am J Cardiol 2011; 107: 1241-1245.

96. Zorlu A, Bektasoglu G, Guven FM, Dogan OT, Gucuk E, Ege MR, et al. Usefulness of admission red cell distribution width as a predictor of early mortality in patients with acute pulmonary embolism. Am J Cardiol 2012; 109: 128-134.

97. Gul M, Uyarel H, Ergelen M, Karacimen D, Ugur M, Turer A, et al. The relationship between red blood cell distribution width and the clinical outcomes in non-ST elevation myocardial infarction and unstable angina pectoris: a 3-year follow-up. Coron Artery Dis 2012; 23: 330-336.

98. Oh J, Kang SM, Won H, Hong N, Kim SY, Park S, et al. Prognostic value of change in red cell distribution width 1 month after discharge in acute decompensated heart failure patients. Circ J 2012; 76: 109-116.

99. Van Craenenbroeck EM, Pelle AJ, Beckers PJ, Possemiers NM, Ramakers C, Vrints CJ, et al. Red cell distribution width as a marker of impaired exercise tolerance in patients with chronic heart failure. Eur J Heart Fail 2012; 14: 54-60.

100. Kim J, Kim YD, Song TJ, Park JH, Lee HS, Nam CM, et al. Red blood cell distribution width is associated with poor clinical outcome in acute cerebral infarction. Thromb Haemost 2012; 108: 349-356.

101. Aung N, Ling HZ, Cheng AS, Aggarwal S, Flint J, Mendonca M, et al. Expansion of the red cell distribution width and evolving iron deficiency as predictors of poor outcome in chronic heart failure. Int J Cardiol 2013.

102. Warwick R, Mediratta N, Shaw M, McShane J, Pullan M, Chalmers J, et al. Red cell distribution width and coronary artery bypass surgery. Eur J Cardiothorac Surg 2012.

103. Ephrem G, Kanei Y. Elevated red blood cell distribution width is associated with higher recourse to coronary artery bypass graft. Cardiology 2012; 123: 135-141.

104. Fatemi O, Paranilam J, Rainow A, Kennedy K, Choi J, Cutlip D, et al. Red cell distribution width is a predictor of mortality in patients undergoing percutaneous coronary intervention. J Thromb Thrombolysis 2013; 35: 57-64.

105. Hong N, Oh J, Kang SM, Kim SY, Won H, Youn JC, et al. Red blood cell distribution width predicts early mortality in patients with acute dyspnea. Clin Chim Acta 2012; 413: 992-997.

106. Tanindi A, Topal FE, Topal F, Celik B. Red cell distribution width in patients with prehypertension and hypertension. Blood Press 2012; 21: 177-181.

107. Lou Y, Wang M, Mao W. Clinical usefulness of measuring red blood cell distribution width in patients with hepatitis B. PLoS One 2012; 7: e37644.

108. Ku NS, Kim HW, Oh HJ, Kim YC, Kim MH, Song JE, et al. Red blood cell distribution width is an independent predictor of mortality in patients with gram-negative bacteremia. Shock 2012; 38: 123-127.

109. Jo YH, Kim K, Lee JH, Kang C, Kim T, Park HM, et al. Red cell distribution width is a prognostic factor in severe sepsis and septic shock. Am J Emerg Med 2013.

110. Braun E, Domany E, Kenig Y, Mazor Y, Makhoul BF, Azzam ZS. Elevated red cell distribution width predicts poor outcome in young patients with community acquired pneumonia. Crit Care 2011; 15: R194.

111. Lee JH, Chung HJ, Kim K, Jo YH, Rhee JE, Kim YJ, et al. Red cell distribution width as a prognostic marker in patients with community-acquired pneumonia. Am J Emerg Med 2013; 31: 72-79.

112. Spell DW, Jones DV, Jr., Harper WF, Bessman JD. The value of a complete blood count in predicting cancer of the colon. Cancer Detect Prev 2004; 28: 37-42.

113. Harmanci O, Kav T, Sivri B. Red cell distribution width can predict intestinal atrophy in selected patients with celiac disease. J Clin Lab Anal 2012; 26: 497-502.

114. Senol K, Saylam B, Kocaay F, Tez M. Red cell distribution width as a predictor of mortality in acute pancreatitis. Am J Emerg Med 2013.

115. Garbharran U, Chinthapalli S, Hopper I, George M, Back DL, Dockery F. Red cell distribution width is an independent predictor of mortality in hip fracture. Age Ageing 2012.

116. Oh HJ, Park JT, Kim JK, Yoo DE, Kim SJ, Han SH, et al. Red blood cell distribution width is an independent predictor of mortality in acute kidney injury patients treated with continuous renal replacement therapy. Nephrol Dial Transplant 2012; 27: 589-594.

117. Perlstein TS, Weuve J, Pfeffer MA, Beckman JA. Red blood cell distribution width and mortality risk in a community-based prospective cohort. Arch Intern Med 2009; 169: 588-594.

118. Patel KV, Ferrucci L, Ershler WB, Longo DL, Guralnik JM. Red blood cell distribution width and the risk of death in middle-aged and older adults. Arch Intern Med 2009; 169: 515-523.

119. Patel KV, Semba RD, Ferrucci L, Newman AB, Fried LP, Wallace RB, et al. Red cell distribution width and mortality in older adults: a meta-analysis. J Gerontol A Biol Sci Med Sci 2010; 65: 258-265.

120. de Gonzalo-Calvo D, de Luxan-Delgado B, Martinez- Camblor P, Rodriguez-Gonzalez S, Garcia-Macia M, Suarez FM, et al. Chronic inflammation as predictor of 1-year hospitalization and mortality in elderly population. Eur J Clin Invest 2012; 42: 1037-1046.

121. Martinez-Velilla N, Ibanez B, Cambra K, Alonso- Renedo J. Red blood cell distribution width, multimorbidity, and the risk of death in hospitalized older patients. Age (Dordr) 2012; 34: 717-723.

122. Malandrino N, Wu WC, Taveira TH, Whitlatch HB, Smith RJ. Association between red blood cell distribution width and macrovascular and microvascular complications in diabetes. Diabetologia 2012; 55: 226-235.

123. Buttarello M, Plebani M. Automated blood cell counts: state of the art. Am J Clin Pathol 2008; 130: 104-116.

124. D’Onofrio G, Kim YR, Schulze S, Lorentz T, Dorner K, Goossens W, et al. Evaluation of the Abbott Cell Dyn 4000 automated fluorescent reticulocyte measurements: comparison with manual, FACScan and Sysmex R1000 methods. Clin Lab Haematol 1997; 19: 253-260.

125. Sica S, Sora F, Laurenti L, Piccirillo N, Salutari P, Chiusolo P, et al. Highly fluorescent reticulocyte count predicts haemopoietic recovery after immunosuppression for severe aplastic anaemia. Clin Lab Haematol 1999; 21: 387-389.

126. Savage RA, Skoog DP, Rabinovitch A. Analytic inaccuracy and imprecision in reticulocyte counting: a preliminary report from the College of American Pathologists Reticulocyte Project. Blood Cells 1985; 11: 97-112.

127. Briggs C, Bain BJ. Basic haematological techniques. In: Bain BJ, Bates I, Laffan MA, Lewis SM, eds. Dacie and Lewis Practical Haematology (ed 11th edition): Churchil Livingstone Elsevier; 2012.

128. Kim A, Park J, Kim M, Lim J, Oh EJ, Kim Y, et al. Correction of pseudoreticulocytosis in leukocytosis samples using the Sysmex XE-2100 analyzer depends on the type and number of white blood cells. Ann Lab Med 2012; 32: 392-398.

129. Lippi G, Pavesi F, Cattabiani C, Avanzini P, Pipitone S. Influence of in vitro hemolysis on nucleated red blood cells and reticulocyte counts. Int J Lab Hematol 2012.

130. Torres A, Sanchez J, Lakomsky D, Serrano J, Alvarez MA, Martin C, et al. Assessment of hematologic progenitor engraftment by complete reticulocyte maturation parameters after autologous and allogeneic hematopoietic stem cell transplantation. Haematologica 2001; 86: 24-29.

131. Noronha JF, De Souza CA, Vigorito AC, Aranha FJ, Zulli R, Miranda EC, et al. Immature reticulocytes as an early predictor of engraftment in autologous and allogeneic bone marrow transplantation. Clin Lab Haematol 2003; 25: 47-54.

132. Yesmin S, Sultana T, Roy CK, Rahman MQ, Ahmed AN. Immature reticulocyte fraction as a predictor of bone marrow recovery in children with acute lymphoblastic leukaemia on remission induction phase. Bangladesh Med Res Counc Bull 2011; 37: 57-60.

133. Mullier F, Lainey E, Fenneteau O, Da Costa L, Schillinger F, Bailly N, et al. Additional erythrocytic and reticulocytic parameters helpful for diagnosis of hereditary spherocytosis: results of a multicentre study. Ann Hematol 2011; 90: 759-768.

134. D’Onofrio G, Tichelli A, Foures C, Theodorsen L. Indicators of haematopoietic recovery after bone marrow transplantation: the role of reticulocyte measurements. Clin Lab Haematol 1996; 18 Suppl 1: 45-53.

135. Grazziutti ML, Dong L, Miceli MH, Cottler-Fox M, Krishna SG, Fassas A, et al. Recovery from neutropenia can be predicted by the immature reticulocyte fraction several days before neutrophil recovery in autologous stem cell transplant recipients. Bone Marrow Transplant 2006; 37: 403-409.

136. Huh J, Moon H, Chung W. Erroneously elevated immature reticulocyte counts in leukemic patients determined using a Sysmex XE-2100 hematology analyzer. Ann Hematol 2007; 86: 759-762.

137. Canals C, Remacha AF, Sarda MP, Piazuelo JM, Royo MT, Romero MA. Clinical utility of the new Sysmex XE 2100 parameter - reticulocyte hemoglobin equivalent - in the diagnosis of anemia. Haematologica 2005; 90: 1133-1134.

138. Brugnara C, Schiller B, Moran J. Reticulocyte hemoglobin equivalent (Ret He) and assessment of iron-deficient states. Clin Lab Haematol 2006; 28: 303-308.

139. Brugnara C, Laufer MR, Friedman AJ, Bridges K, Platt O. Reticulocyte hemoglobin content (CHr): early indicator of iron deficiency and response to therapy. Blood 1994; 83: 3100-3101.

140. Mast AE, Blinder MA, Dietzen DJ. Reticulocyte hemoglobin content. Am J Hematol 2008; 83: 307-310.

141. Brugnara C, Zelmanovic D, Sorette M, Ballas SK, Platt O. Reticulocyte hemoglobin: an integrated parameter for evaluation of erythropoietic activity. Am J Clin Pathol 1997; 108: 133-142.

142. Brugnara C. Use of reticulocyte cellular indices in the diagnosis and treatment of hematological disorders. Int J Clin Lab Res 1998; 28: 1-11.

143. Brugnara C, Zurakowski D, DiCanzio J, Boyd T, Platt O. Reticulocyte hemoglobin content to diagnose iron deficiency in children. Jama 1999; 281: 2225-2230.

144. Brugnara C. Reticulocyte cellular indices: a new approach in the diagnosis of anemias and monitoring of erythropoietic function. Crit Rev Clin Lab Sci 2000; 37: 93-130.

145. Ageeli AA, Algahtani FH, Alsaeed AH. Reticulocyte Hemoglobin Content and Iron Deficiency: A Retrospective Study in Adults. Genet Test Mol Biomarkers 2013.

146. Fishbane S, Galgano C, Langley RC, Jr., Canfield W, Maesaka JK. Reticulocyte hemoglobin content in the evaluation of iron status of hemodialysis patients. Kidney Int 1997; 52: 217-222.

147. Fishbane S, Shapiro W, Dutka P, Valenzuela OF, Fau bert J. A randomized trial of iron deficiency testing strategies in hemodialysis patients. Kidney Int 2001; 60: 2406-2411.

148. Tsuchiya K, Saito M, Okano-Sugiyama H, Nihei H, Ando M, Teramura M, et al. Monitoring the content of reticulocyte hemoglobin (CHr) as the progression of anemia in nondialysis chronic renal failure (CRF) patients. Ren Fail 2005; 27: 59-65.

149. Garzia M, Di Mario A, Ferraro E, Tazza L, Rossi E, Luciani G, et al. Reticulocyte Hemoglobin Equivalent: An Indicator of Reduced Iron Availability in Chronic Kidney Diseases during Erythropoietin Therapy. Lab Hematol 2007; 13: 6-11.

150. Eguchi A, Mochizuki T, Tsukada M, Kataoka K, Hamaguchi Y, Oguni S, et al. Serum hepcidin levels and reticulocyte hemoglobin concentrations as indicators of the iron status of peritoneal dialysis patients. Int J Nephrol 2012; 2012: 239476.

151. Urrechaga E, Borque L, Escanero JF. Erythrocyte and reticulocyte indices in the assessment of erythropoiesis activity and iron availability. Int J Lab Hematol 2012.

152. Parisotto R, Wu M, Ashenden MJ, Emslie KR, Gore CJ, Howe C, et al. Detection of recombinant human erythropoietin abuse in athletes utilizing markers of altered erythropoiesis. Haematologica 2001; 86: 128- 137.

153. Fallon KE. Utility of hematological and iron-related screening in elite athletes. Clin J Sport Med 2004; 14: 145-152.

154. Buttarello M, Temporin V, Ceravolo R, Farina G, Bulian P. The new reticulocyte parameter (RET-Y) of the Sysmex XE 2100: its use in the diagnosis and monitoring of posttreatment sideropenic anemia. Am J Clin Pathol 2004; 121: 489-495.

155. Giles I. Hematology automation aids in athletic testing. MLO Med Lab Obs 2005; 37: 22, 24.

156. Fujimoto K. Principles of measurement in hematology analyzers manufactured by Sysmex Corporation. Sysmex J Int 1999; 9: 31-44.

157. Coulter DB, Reece WO, Small LL, Snodgrass RR. Continuous recording of erythrocyte counts and hemoglobin concentrations in the dog. Lab Anim Sci 1972; 22: 210-215.

158. Margolis KL, Manson JE, Greenland P, Rodabough RJ, Bray PF, Safford M, et al. Leukocyte count as a predictor of cardiovascular events and mortality in postmenopausal women: the Women’s Health Initiative Observational Study. Arch Intern Med 2005; 165: 500-508.

159. Jia EZ, Yang ZJ, Yuan B, Zang XL, Wang RH, Zhu TB, et al. Relationship between leukocyte count and angiographical characteristics of coronary atherosclerosis. Acta Pharmacol Sin 2005; 26: 1057-1062.

160. Futterman LG, Lemberg L. The leukocyte count, a measure and predictor of coronary events. Am J Crit Care 2007; 16: 401-404.

161. Ndrepepa G, Braun S, Iijima R, Keta D, Byrne RA, Schulz S, et al. Total leucocyte count, but not C-reactive protein, predicts 1-year mortality in patients with acute coronary syndromes treated with percutaneous coronary intervention. Clin Sci (Lond) 2009; 116: 651-658.

162. O Hartaigh B, Bosch JA, Thomas GN, Lord JM, Pilz S, Loerbroks A, et al. Which leukocyte subsets predict cardiovascular mortality? From the LUdwigshafen RIsk and Cardiovascular Health (LURIC) Study. Atherosclerosis 2012; 224: 161-169.

163. Angeli F, Angeli E, Ambrosio G, Mazzotta G, Reboldi G, Verdecchia P. Neutrophil count for the identification of postmenopausal hypertensive women at increased cardiovascular risk. Obstet Gynecol 2010; 115: 695-703.

164. Pierre RV. Peripheral blood film review. The demise of the eyecount leukocyte differential. Clin Lab Med 2002; 22: 279-297.

165. Castro OL, Haddy TB, Rana SR. Age- and sex-related blood cell values in healthy black Americans. Public Health Rep 1987; 102: 232-237.

166. Wolach B, Ashkenazi M, Grossmann R, Gavrieli R, Friedman Z, Bashan N, et al. Diurnal fluctuation of leukocyte G6PD activity. A possible explanation for the normal neutrophil bactericidal activity and the low incidence of pyogenic infections in patients with severe G6PD deficiency in Israel. Pediatr Res 2004; 55: 807-813.

167. Lewis SM. Reference ranges and normal value. In: Lewis SM, Bain BJ, Bates I, eds. Dacie and Lewis practical haematology (ed 5th edition). Philadelphia, PA, USA: Churchill Livingston; 2006: 11-24.

168. Balloch AJ, Cauchi MN. Reference ranges for haematology parameters in pregnancy derived from patient populations. Clin Lab Haematol 1993; 15: 7-14.

169. Taft EG, Grossman J, Abraham GN, Leddy JP, Lichtman MA. Pseudoleukocytosis due to cryoprotein crystals. Am J Clin Pathol 1973; 60: 669-671.

170. Shah PC, Rao K, Noble V, Kumaraiah V, Patel AR. Transient pseudoleukocytosis caused by cryocrystalglobulinemia. Arch Pathol Lab Med 1978; 102: 172- 173.

171. Luzar MJ, Camisa C, Neff JC. Essential mixed cryoglobulinemia (type II) with pseudoleukocytosis. Arthritis Rheum 1984; 27: 353-355.

172. Zandecki M, Dupriez B, Fenaux P, Jude B, Watel A, Dracon M, et al. Cytological and ultrastructural assessment of free crystals or precipitates associated with pseudoleukocytosis and pseudothrombocytosis in cryoglobulinemia. Nouv Rev Fr Hematol 1989; 31: 397-402.

173. Gulati G, Piao Y, Song A. Interference by cryoproteins in the blood with automated CBCs. Lab Med 1995; 26: 138-142.

174. von Schenck H. Cryoglobulin detected through spurious increases in automated leukocyte counts. Clin Chem 1985; 31: 1086-1087.

175. Di Giovanni S, De Matteis MA, Ciocca D, Cirella MP, Nanni Costa MP. Pseudothrombocytosis and pseudoleukocytosis in a case of essential mixed cryoglobulinemia (type II). Clin Exp Rheumatol 1986; 4: 143-145.

176. Fohlen-Walter A, Jacob C, Lecompte T, Lesesve JF. Laboratory identification of cryoglobulinemia from automated blood cell counts, fresh blood samples, and blood films. Am J Clin Pathol 2002; 117: 606-614.

177. Smith SB, Arkin C. Cryofibrinogenemia: incidence, clinical correlations, and a review of the literature. Am J Clin Pathol 1972; 58: 524-530.

178. Savage RA. Pseudoleukocytosis due to EDTA-induced platelet clumping. Am J Clin Pathol 1984; 81: 317- 322.

179. Savage RA. Analytic inaccuracy resulting from hematology specimen characteristics. Three cases of clinically misleading artifacts affecting white blood cell and platelet counts. Am J Clin Pathol 1989; 92: 295-299.

180. Berkman N, Michaeli Y, Or R, Eldor A. EDTA-dependent pseudothrombocytopenia: a clinical study of 18 patients and a review of the literature. Am J Hematol 1991; 36: 195-201.

181. Racchi O, Rapezzi D. Megathrombocytes and spurious thrombocytopenia. Eur J Haematol 2001; 66: 140-141.

182. Robbins SH, Conly MA, Oettinger J. Cold-induced granulocyte agglutination. A cause of pseudoleukopenia. Arch Pathol Lab Med 1991; 115: 155-157.

183. Pewarchuk W, VanderBoom J, Blajchman MA. Pseudopolycythemia, pseudothrombocytopenia, and pseudoleukopenia due to overfilling of blood collection vacuum tubes. Arch Pathol Lab Med 1992; 116: 90-92.

184. Sanders C. Fragility of leucocytes in chronic lymphatic leukaemia. J Clin Pathol 1966; 19: 526.

185. Luke RG, Koepke JA, Siegel RR. The effects of immunosuppressive drugs and uremia on automated leukocyte counts. Am J Clin Pathol 1971; 56: 503-507.

186. Epstein HD, Kruskall MS. Spurious leukopenia due to in vitro granulocyte aggregation. Am J Clin Pathol 1988; 89: 652-655.

187. Rohr LR, Rivers FM. Spurious automated leukopenia due to in vitro granulocyte aggregation. Am J Clin Pathol 1990; 93: 572-574.

188. Hillyer CD, Knopf AN, Berkman EM. EDTA-dependent leukoagglutination. Am J Clin Pathol 1990; 94: 458- 461.

189. O’Connor BM, Shastri KA, Logue GL. Spurious neutropenia: ethylenediaminetetraacetate-dependent in vitro neutrophil agglutination. N Y State J Med 1991; 91: 455-456.

190. Galifi M, Schinella M, Nicoli M, Lippi G. Instrumental reports and effect of anticoagulants in a case of neutrophil agglutination in vitro. Haematologica 1993; 78: 364-370.

191. Deol I, Hernandez AM, Pierre RV. Ethylenediamine tetraacetic acid-associated leukoagglutination. Am J Clin Pathol 1995; 103: 338-340.

192. Glasser L. Pseudo-neutropenia secondary to leukoagglutination. Am J Hematol 2005; 80: 147.

193. Moraglio D, Banfi G, Arnelli A. Association of pseudothrombocytopenia and pseudoleukopenia: evidence for different pathogenic mechanisms. Scand J Clin Lab Invest 1994; 54: 257-265.

194. Zelster D, Fusman R, Chapman J, Rotstein R, Shapira I, Elkayam O, et al. Increased leukocyte aggregation induced by gamma-globulin: a clue to the presence of pseudoleukopenia. Am J Med Sci 2000; 320: 177- 182.

195. Waddell CC, Shapiro DM, Min KW. Spuriously low automated blood counts in mucin-producing adenocarcinoma. Am J Clin Pathol 1977; 67: 115-116.

196. Iddles C, Taylor J, Cole R, Hill FGH. Evaluation of the immature granulocyte count in the diagnosis of sepsis using the Sysmex XE-2100 analyser. Sysmex J Int 2007; 17 (Suppl 1): 20-29.

197. Briggs C, Kunka S, Fujimoto H, Hamaguchi Y, Davis BH, Machin SJ. Evaluation of immature granulocyte counts by the XE-IG master: upgraded software for the XE-2100 automated hematology analyzer. Lab Hematol 2003; 9: 117-124.

198. Field D, Taube E, Heumann S. Performance evaluation of the immature granulocyte parameter on the Sysmex XE-2100 automated hematology analyzer. Lab Hematol 2006; 12: 11-14.

199. Nigro KG, O’Riordan M, Molloy EJ, Walsh MC, Sandhaus LM. Performance of an automated immature granulocyte count as a predictor of neonatal sepsis. Am J Clin Pathol 2005; 123: 618-624.

200. Farias MG, Garcia MP, Cagliari CR. Evaluation of the immature myeloid information (IMI) by Sysmex XE 2100(R) hematology analyzer in the identification of blasts of myeloid lineage. Clin Chem Lab Med 2012; 50: 1861-1864.

201. Pekelharing JM, Hauss O, de Jonge R, Lokhoff J, Sodikromo J, Spaans M, et al. Haematology reference intervals for established and novel parameters in healthy adults. Diagnostic perspectives 2010; 1: 1-11.

202. Ansari-Lari MA, Kickler TS, Borowitz MJ. Immature granulocyte measurement using the Sysmex XE- 2100. Relationship to infection and sepsis. Am J Clin Pathol 2003; 120: 795-799.

203. Cimenti C, Erwa W, Herkner KR, Kasper DC, Muller W, Resch B. The predictive value of immature granulocyte count and immature myeloid information in the diagnosis of neonatal sepsis. Clin Chem Lab Med 2012; 50: 1429-1432.

204. Nierhaus A, Klatte S, Linssen J, Eismann NM, Wichmann D, Hedke J, et al. Revisiting the white blood cell count: immature granulocytes count as a diagnostic marker to discriminate between SIRS and sepsis - a prospective, observational study. BMC Immunol 2013; 14: 8.

205. George TI. Malignant or benign leukocytosis. Hematology Am Soc Hematol Educ Program 2012; 2012: 475-484.

206. Fernandes B, Hamaguchi Y. Automated enumeration of immature granulocytes. Am J Clin Pathol 2007; 128: 454-463.

207. Schaffer M, Rowan RM. The clinical relevance of nucleated red blood cell counts. Sysmex J Int 2010; 10: 59-63.

208. Gulati G, Behling E, Kocher W, Schwarting R. An evaluation of the performance of Sysmex XE-2100 in enumerating nucleated red cells in peripheral blood. Arch Pathol Lab Med 2007; 131: 1077-1083.

209. Danise P, Maconi M, Barrella F, Di Palma A, Avino D, Rovetti A, et al. Evaluation of nucleated red blood cells in the peripheral blood of hematological diseases. Clin Chem Lab Med 2012; 50: 357-360.

210. Gasparovic VE, Ahmetasevic SG, Colic A. Nucleated red blood cells count as first prognostic marker for adverse neonatal outcome in severe preeclamptic pregnancies. Coll Antropol 2012; 36: 853-857.

211. Desai S, Jones SL, Turner KL, Hall J, Moore LJ. Nucleated red blood cells are associated with a higher mortality rate in patients with surgical sepsis. Surg Infect (Larchmt) 2012; 13: 360-365.

212. Kuert S, Holland-Letz T, Friese J, Stachon A. Association of nucleated red blood cells in blood and arterial oxygen partial tension. Clin Chem Lab Med 2011; 49: 257-263.

213. Shah R, Reddy S, Horst HM, Stassinopoulos J, Jordan J, Rubinfeld I. Getting back to zero with nucleated red blood cells: following trends is not necessarily a bad thing. Am J Surg 2012; 203: 343-345; discussion 345- 346.

214. de Keijzer MH, van der Meer W. Automated counting of nucleated red blood cells in blood samples of newborns. Clin Lab Haematol 2002; 24: 343-345.

215. Linssen J, Jennissen V. Identification of the high fluorescence lymphocyte (HFL) count on the XE-5000 with efficient multichannel messaging (eMM) as antibody synthesizing cells, c. q. plasma cells. Sysmex J Int 2009; 19: 19-25.

216. Linssen J, Jennissen V, Hildmann J, Reisinger E, Schindler J, Malchau G, et al. Identification and quantification of high fluorescence-stained lymphocytes as antibody synthesizing/secreting cells using the automated routine hematology analyzer XE- 2100. Cytometry B Clin Cytom 2007; 72: 157-166.

217. Rowan RM, Linssen J. A picture is worth a thousand words. Sysmex J Int 2005; 15: 27-38.

218. Herklotz R, Huber R. Precision and accuracy of the leukocyte differential on the Sysmex XE 2100. Sysmex J Int 2001; 11: 8-21.

219. Campuzano-Zuluaga G, Alvarez-Sanchez G, Escobar- Gallo GE, Valencia-Zuluaga LM, Rios-Orrego AM, Pabon-Vidal A, et al. Design of malaria diagnostic criteria for the Sysmex XE-2100 hematology analyzer. Am J Trop Med Hyg 2010; 82: 402-411.

220. Campuzano-Zuluaga G, Hanscheid T, Grobusch MP. Automated haematology analysis to diagnose malaria. Malar J 2010; 9: 346.

221. Mohapatra S, Samantaray JC, Arulselvi S, Panda J, Munot K, Saxena R. Automated detection of malaria with haematology analyzer Sysmex XE-2100. Indian J Med Sci 2011; 65: 26-31.

222. Huh J, Jung J, Yoon H, Chung W. Pseudoeosinophilia associated with malaria infection determined in the Sysmex XE-2100 hematology analyzer. Ann Hematol 2005; 84: 400-402.

223. Huh HJ, Oh GY, Huh JW, Chae SL. Malaria detection with the Sysmex XE-2100 hematology analyzer using pseudoeosinophilia and abnormal WBC scattergram. Ann Hematol 2008; 87: 755-759.

224. Inoue H. Overview of automated hematology analyzer XE-2100. Sysmex J Int 1999; 9: 58-64.

225. Lombarts AJ, de Kieviet W. Recognition and prevention of pseudothrombocytopenia and concomitant pseudoleukocytosis. Am J Clin Pathol 1988; 89: 634- 639.

226. Cohen AM, Cycowitz Z, Mittelman M, Lewinski UH, Gardyn J. The incidence of pseudothrombocytopenia in automatic blood analyzers. Haematologia (Budap) 2000; 30: 117-121.

227. Matarazzo M, Conturso V, Di Martino M, Chiurazzi F, Guida G, Morante R. EDTA-dependent pseudothrombocytopenia in a case of liver cirrhosis. Panminerva Med 2000; 42: 155-157.

228. Mori M, Kudo H, Yoshitake S, Ito K, Shinguu C, Noguchi T. Transient EDTA-dependent pseudothrombocytopenia in a patient with sepsis. Intensive Care Med 2000; 26: 218-220.

229. Hsieh AT, Chao TY, Chen YC. Pseudothrombocytopenia associated with infectious mononucleosis. Arch Pathol Lab Med 2003; 127: e17-18.

230. Tu CH, Yang S. Olanzapine-induced EDTA-dependent pseudothrombocytopenia. Psychosomatics 2002; 43: 421-423.

231. Yoshikawa H. EDTA-dependent pseudothrombocytopenia induced by valproic acid. Neurology 2003; 61: 579-580.

232. Christensen RD, Sola MC, Rimsza LM, McMahan MJ, Calhoun DA. Pseudothrombocytopenia in a preterm neonate. Pediatrics 2004; 114: 273-275.

233. Ahmed P, Minnich V, Michael JM. Platelet satellitosis with spurious thrombocytopenia and neutropenia. Am J Clin Pathol 1978; 69: 473-474.

234. Bodensteiner D, Talley R, Rosenfeld C. Platelet satellitism: a possible mechanism. South Med J 1987; 80: 459-461.

235. Cunningham VL, Brandt JT. Spurious thrombocytopenia due to EDTA-independent cold-reactive agglutinins. Am J Clin Pathol 1992; 97: 359-362.

236. Shimasaki AK, Fujita K, Fujio S, Sakurabayashi I. Pseudoleukocytosis without pseudothrombocytopenia induced by the interaction of EDTA and Ig- G(2)-kappa M-protein. Clin Chim Acta 2000; 299: 119-128.

237. Kjeldsberg CR. Letter: Platelet satellitism. N Engl J Med 1974; 290: 165.

238. Greipp PR, Gralnick HR. Platelet to leukocyte adherence phenomena associated with thrombocytopenia. Blood 1976; 47: 513-521.

239. Lazo-Langner A, Piedras J, Romero-Lagarza P, Lome-Maldonado C, Sánchez-Guerrero J, López-Karpovitch X. Platelet satellitism, spurious neutropenia, and cutaneous vasculitis: casual or causal association? Am J Hematol 2002; 70: 246-249.

240. Sultan S, Muhammad Irfan S. Platelet satellitism: a spurious cause of thrombocytopenia in chronic liver disease. Eur J Haematol 2012.

241. Solanki DL, Blackburn BC. Spurious thrombocytopenia during pregnancy. Obstet Gynecol 1985; 65: 14S-17S.

242. Hatzipantelis ES, Tsantali H, Athanassiou-Metaxa M, Avramidou K, Zambul D, Gombakis N. Hereditary giant platelet disorder presented as pseudothrombocytopenia. Eur J Haematol 2001; 67: 330-331.

243. Onder O, Weinstein A, Hoyer LW. Pseudothrombocytopenia caused by platelet agglutinins that are reactive in blood anticoagulated with chelating agents. Blood 1980; 56: 177-182.

244. Poskitt TR, Poskitt PK. Spurious thrombocytopenia produced by the interaction of rheumatoid factor with antiplatelet antibody. Am J Hematol 1985; 18: 207-211.

245. Perez-Vila ME, Pedro C, Mellibovsky L, Woessner S, Serrano S, Florensa L. Spurious thrombocytosis associated with cryoglobulinaemia: a case report. Haematologica 2002; 87: ELT39.

246. Ballard HS, Sidhu G. Cytoplasmic fragments causing spurious platelet counts in hairy cell leukemia: ultrastructural characterization. Arch Intern Med 1981; 141: 942-944.

247. Stass SA, Holloway ML, Peterson V, Creegan WJ, Gallivan M, Schumacher HR. Cytoplasmic fragments causing spurious platelet counts in the leukemic phase of poorly differentiated lymphocytic lymphoma. Am J Clin Pathol 1979; 71: 128-128.

248. Armitage JO, Goeken JA, Feagler JR. Spurious elevation of the platelet count in acute leukemia. Jama 1978; 239: 433-434.

249. Hanker JS, Giammara BL. Neutrophil pseudoplatelets: their discrimination by myeloperoxidase demonstration. Science 1983; 220: 415-417.

250. Payne BA, Pierre RV. Pseudothrombocytopenia: a laboratory artifact with potentially serious consequences. Mayo Clin Proc 1984; 59: 123-125.

251. Li S, Salhany KE. Spurious elevation of automated platelet counts in secondary acute monocytic leukemia associated with tumor lysis syndrome. Arch Pathol Lab Med 1999; 123: 1111-1114.

252. Bonifazi F, Stanzani M, Bandini G. A case of pseudothrombocytosis. Haematologica 1999; 84: 275.

253. Morton BD, Orringer EP, LaHart LA, Stass SA. Pappenheimer bodies. An additional cause for a spurious platelet count. Am J Clin Pathol 1980; 74: 310-311.

254. Gloster ES, Strauss RA, Jimenez JF, Neuberg RW, Berry DH, Turner EJ. Spurious elevated platelet counts associated with bacteremia. Am J Hematol 1985; 18: 329-332.

255. Bain BJ, Arnold JA, Jowzi Z. Spurious automated platelet count. Am J Clin Pathol 2004; 122: 316; author reply 316.

256. Threatte GA. Usefulness of the mean platelet volume. Clin Lab Med 1993; 13: 937-950.

257. Jackson SR, Carter JM. Platelet volume: laboratory measurement and clinical application. Blood Rev 1993; 7: 104-113.

258. International Committee for Standardization in Haematology. Recommendations for the analysis of red cell, white cell and platelet size distribution curves: I. General principles. J Clin Pathol 1982; 35: 1320-1322.

259. Osselaer JC, Jamart J, Scheiff JM. Platelet distribution width for differential diagnosis of thrombocytosis. Clin Chem 1997; 43: 1072-1076.

260. Trowbridge EA, Martin JF. The platelet volume distribution: a signature of the prethrombotic state in coronary heart disease? Thromb Haemost 1987; 58: 714-717.

261. Bath PM, Butterworth RJ. Platelet size: measurement, physiology and vascular disease. Blood Coagul Fibrinolysis 1996; 7: 157-161.

262. Wiwanitkit V. Plateletcrit, mean platelet volume, platelet distribution width: its expected values and correlation with parallel red blood cell parameters. Clin Appl Thromb Hemost 2004; 10: 175-178.

263. Kaito K, Otsubo H, Usui N, Yoshida M, Tanno J, Kurihara E, et al. Platelet size deviation width, platelet large cell ratio, and mean platelet volume have sufficient sensitivity and specificity in the diagnosis of immune thrombocytopenia. Br J Haematol 2005; 128: 698-702.

264. Bessman JD, Gardner FH. Platelet size in thrombocytopenia due to sepsis. Surg Gynecol Obstet 1983; 156: 177-180.

265. Bessman JD, Gilmer PR, Gardner FH. Use of mean platelet volume improves detection of platelet disorders. Blood Cells 1985; 11: 127-135.

266. Macchi I, Chamlian V, Sadoun A, Le Dirach A, Guilhot J, Guilhot F, et al. Comparison of reticulated platelet count and mean platelet volume determination in the evaluation of bone marrow recovery after aplastic chemotherapy. Eur J Haematol 2002; 69: 152-157.

267. Bessman JD. The relation of megakaryocyte ploidy to platelet volume. Am J Hematol 1984; 16: 161-170.

268. Berg SL, Steuber CP, Poplack DG, 1155-1162. Clinical manifestations of acute lymphoblastic leukemia. In: Hoffman R, Benz EJ, Shattil SJ, Furie B, Cohen HJ, Silberstein LE, et al., eds. Hematology Basic principles and practice (ed 4th edition). Philadelphia, Pennsylvania: Elseiver, Inc; 2005: 1155-1162.

269. Miller KB, Daoust PR. Clinical manifestations of acute myeloid leukemia. In: Hoffman R, Benz EJ, Shattil SJ, Furie B, Cohen HJ, Silberstein LE, et al., eds. Hematology Basic principles and practice (ed 4th edition). Philadelphia, Pennsylvania: Elseiver, Inc; 2005: 1071- 1097.

270. De Angelo DJ, R.M. S. Myelodysplastic syndromes: Biology and treatment. In: Hoffman R, Benz EJ, Shattil SJ, Furie B, Cohen HJ, Silberstein LE, et al., eds. Hematology Basic principles and practice (ed 4th edition). Philadelphia, Pennsylvania: Elseiver, Inc; 2005: 1195- 1208.

271. Patel DV, Rai KR. Chronic lymphocytic leukemia. In: Hoffman R, Benz EJ, Shattil SJ, Furie B, Cohen HJ, Silberstein LE, et al., eds. Hematology Basic principles and practice (ed 4th edition). Philadelphia, Pennsylvania: Elseiver, Inc; 2005: 1437-1454.

272. Zakarija A, Peterson LC, Tallman MS. Hairy cell leukemia. In: Hoffman R, Benz EJ, Shattil SJ, Furie B, Cohen HJ, Silberstein LE, et al., eds. Hematology Basic principles and practice (ed 4th edition). Philadelphia, Pennsylvania: Elseiver, Inc; 2005: 1455-1465.

273. Karpatkin S, Freedman ML. Hypersplenic thrombocytopenia differentiated from increased peripheral destruction by platelet volume. Ann Intern Med 1978; 89: 200-203.

274. Levin J, Bessman JD. The inverse relation between platelet volume and platelet number. Abnormalities in hematologic disease and evidence that platelet size does not correlate with platelet age. J Lab Clin Med 1983; 101: 295-307.

275. Threatte GA, Adrados C, Ebbe S, Brecher G. Mean platelet volume: the need for a reference method. Am J Clin Pathol 1984; 81: 769-772.

276. Bull BS, Zucker MB. Changes in platelet volume produced by temperature, metabolic inhibitors, and aggregating agents. Proc Soc Exp Biol Med 1965; 120: 296-301.

277. Bessman JD, Williams LJ, Gilmer PR, Jr. Platelet size in health and hematologic disease. Am J Clin Pathol 1982; 78: 150-153.

278. Nelson RB, 3rd, Kehl D. Electronically determined platelet indices in thrombocytopenic patients. Cancer 1981; 48: 954-956.

279. Beyan C, Kaptan K, Ifran A. Platelet count, mean platelet volume, platelet distribution width, and plateletcrit do not correlate with optical platelet aggregation responses in healthy volunteers. J Thromb Thrombolysis 2006; 22: 161-164.

280. Ntaios G, Gurer O, Faouzi M, Aubert C, Michel P. Hypertension is an indepedent predictor of mean platelet volume in patients with acute ischemic stroke. Intern Med J 2010.

281. Babu E, Basu D. Platelet large cell ratio in the differential diagnosis of abnormal platelet counts. Indian J Pathol Microbiol 2004; 47: 202-205.

282. Kabutomori O, Kanakura Y, Iwatani Y. Characteristic changes in platelet-large cell ratio, lactate dehydrogenase and C-reactive protein in thrombocytosis-related diseases. Acta Haematol 2007; 118: 84-87.

283. Abe Y, Wada H, Tomatsu H, Sakaguchi A, Nishioka J, Yabu Y, et al. A simple technique to determine thrombopoiesis level using immature platelet fraction (IPF). Thromb Res 2006; 118: 463-469.

284. Abe Y, Wada H, Sakakura M, Nishioka J, Tomatsu H, Hamaguchi Y, et al. Usefulness of fully automated measurement of reticulated platelets using whole blood. Clin Appl Thromb Hemost 2005; 11: 263-270.

285. Ault KA, Rinder HM, Mitchell J, Carmody MB, Vary CP, Hillman RS. The significance of platelets with increased RNA content (reticulated platelets). A measure of the rate of thrombopoiesis. Am J Clin Pathol 1992; 98: 637-646.

286. Richards EM, Baglin TP. Quantitation of reticulated platelets: methodology and clinical application. Br J Haematol 1995; 91: 445-451.

287. Campuzano-Maya G. Trombocitopenia: más importante que encontrarla es saber porqué se presenta. Medicina & Laboratorio 2007; 13: 111-125.

288. Strauss G, Vollert C, von Stackelberg A, Weimann A, Gaedicke G, Schulze H. Immature platelet count: a simple parameter for distinguishing thrombocytopenia in pediatric acute lymphocytic leukemia from immune thrombocytopenia. Pediatr Blood Cancer 2011; 57: 641-647.

289. Briggs C, Kunka S, Hart D, Oguni S, Machin SJ. Assessment of an immature platelet fraction (IPF) in peripheral thrombocytopenia. Br J Haematol 2004; 126: 93-99.

290. Wang C, Smith BR, Ault KA, Rinder HM. Reticulated platelets predict platelet count recovery following chemotherapy. Transfusion 2002; 42: 368-374.

291. Takami A, Shibayama M, Orito M, Omote M, Okumura H, Yamashita T, et al. Immature platelet fraction for prediction of platelet engraftment after allogeneic stem cell transplantation. Bone Marrow Transplant 2007; 39: 501-507.

292. Stohlawetz P, Stiegler G, Knobl P, Hocker P, Panzer S. The rise of reticulated platelets after intensive chemotherapy for AML reduces the need for platelet transfusions. Ann Hematol 1999; 78: 271-273.

293. Nakamura T, Uchiyama S, Yamazaki M, Okubo K, Takakuwa Y, Iwata M. Flow cytometric analysis of reticulated platelets in patients with ischemic stroke. Thromb Res 2002; 106: 171-177.

294. McCabe DJ, Harrison P, Sidhu PS, Brown MM, Machin SJ. Circulating reticulated platelets in the early and late phases after ischaemic stroke and transient ischaemic attack. Br J Haematol 2004; 126: 861- 869.

295. Lakkis N, Dokainish H, Abuzahra M, Tsyboulev V, Jorgensen J, De Leon AP, et al. Reticulated platelets in acute coronary syndrome: a marker of platelet activity. J Am Coll Cardiol 2004; 44: 2091-2093.

296. Di Mario A, Garzia M, Leone F, Arcangeli A, Pagano L, Zini G. Immature platelet fraction (IPF) in hospitalized patients with neutrophilia and suspected bacterial infection. J Infect 2009; 59: 201-206.

297. Kienast J, Schmitz G. Flow cytometric analysis of thiazole orange uptake by platelets: a diagnostic aid in the evaluation of thrombocytopenic disorders. Blood 1990; 75: 116-121.

298. O’Malley CJ, Rasko JE, Basser RL, McGrath KM, Cebon J, Grigg AP, et al. Administration of pegylated recombinant human megakaryocyte growth and development factor to humans stimulates the production of functional platelets that show no evidence of in vivo activation. Blood 1996; 88: 3288-3298.

299. Noronha JF, Costa FF, Saad ST, Lorand-Metze IG, Grotto HZ. Evaluation of reticulated platelets in patients with sickle cell diseases. Thromb Res 2007.

300. Peterson P, Blomberg DJ, Rabinovitch A, Cornbleet PJ, Hematology and Clinical Microscopy Resource Committee of the College of American Pathologists. Physician review of the peripheral blood smear: when and why. An opinion. Lab Hematol 2001; 7: 157-179

301. Barnes PW, McFadden SL, Machin SJ, Simson E. The international consensus group for hematology review: suggested criteria for action following automated CBC and WBC differential analysis. Lab Hematol 2005; 11: 83-90.

302. Gulati G, Song J, Florea AD, Gong J. Purpose and criteria for blood smear scan, blood smear examination, and blood smear review. Ann Lab Med 2013; 33: 1-7.

303. Campuzano-Maya G. Editorial: Los contadores de células: son excelentes pero no son perfectos. Medicina & Laboratorio 2005; 11: 309-310.