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Medicina & Laboratorio

2012, Número 11-12

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Medicina & Laboratorio 2012; 18 (11-12)


Tinción de Gram de tejido: alcances y limitaciones

Jiménez TGA, Vélez HA

Idioma: Español
Referencias bibliográficas: 96
Paginas: 557-573
Archivo PDF: 382.29 Kb.


RESUMEN

La identificación de microorganismos en tejido es esencial para reconocer un proceso infeccioso. Inicialmente, mediante la coloración de hematoxilina-eosina, se puede identificar el patrón de inflamación asociado y luego, a través de tinciones basadas en plata y la tinción de Gram de tejido se visualizan los microorganismos. La tinción de Gram no solo sirve para bacterias, sino también para algunos hongos y parásitos; no obstante, esta técnica tiene algunos inconvenientes, como la contaminación con otros microorganismos y la imposibilidad de visualizar algunas bacterias, entre ellas Legionella pneumophila, Leptospira spp y Bartonella spp. En este artículo de revisión se describirán los fundamentos del Gram de tejido, su contribución en el diagnóstico de infecciones como herramienta adicional para el reconocimiento de microorganismos, y sus limitaciones.


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