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2006, Número 2

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Vet Mex 2006; 37 (2)


Aislamiento y caracterización del gen ORF5 del virus del síndrome reproductivo y respiratorio porcino (PRRS) en México

Macías MJ, Yépiz-Plascencia G, Osorio F, Pinelli-Saavedra A, Reyes-Leyva J, Hernández J

Idioma: Español/Inglés
Referencias bibliográficas: 31
Paginas: 197-208
Archivo PDF: 348.16 Kb.


RESUMEN

El virus del síndrome reproductivo y respiratorio porcino (PRRS, por sus siglas en inglés) representa una amenaza de la industria porcina nacional e internacional. Sus propiedades genéticas, antigénicas y su capacidad para modular el sistema inmune, son algunas características que han dificultado su control. Tanto en México como en el mundo, los problemas asociados con esta enfermedad generan cuantiosas pérdidas a la industria porcícola. Este estudio describe el aislamiento y caracterización de dicho virus obtenido a partir de muestras de suero de cerdos de una granja ubicada en Sonora, México. El virus aislado se identificó mediante una RT-PCR anidada del gen ORF-6 y por el efecto citopático en células MARC-145. El virus aislado se inoculó en cerdos negativos, los cuales presentaron viremia y respuesta de anticuerpos y células mononucleares (CMN) después de la inoculación. Para determinar el genotipo del virus aislado, se determinó la secuencia del gen ORF-5, la cual fue 88% y 87% similar al virus de referencia americano (VR-2332) y al virus vivo modificado vacunal (MLV), respectivamente. Estos estudios son esenciales para desarrollar estrategias encaminadas al control de la enfermedad en México.


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