Corona MJD, Pérez SE, Sánchez MV

Idioma: Español
Referencias bibliográficas: 21
Paginas: 23-30
Archivo PDF: 297.62 Kb.

RESUMEN

El estudio de la microbiota bacteriana asociada con periodontitis es esencial para desarrollar herramientas de diagnóstico y eficacia en las terapias, por esta razón el objetivo de este estudio fue identificar bacterias asociadas con periodontitis. Nosotros amplificamos el gen 16S rARN por reacción en cadena de polimerasa (PCR) y secuenciamos para investigar y comparar muestras subgingivales obtenidas de individuos sanos y pacientes con periodontitis moderada y severa. Identificamos Stenotrophomonas maltophilia, Porphyromonas gingivalis, Pseudomonas sp., Fusobacterium nucleatum, Haemophilus sp., Aggregatibacter sp. y Prevotella intermedia. P. gingivalis y F. nucleatum se asociaron con ambas periodontitis y Aggregatibacter sp. se asoció con periodontitis severa. Los resultados presentados aquí podrían contribuir en la toma de decisiones clínicas de la periodontitis.

REFERENCIAS (EN ESTE ARTÍCULO)

1. Darveau RP. Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol. 2010; 8 (7): 481-490.

2. Pihlstrom BL, Michalowicz BS, Johnson NW. Periodontal diseases. Lancet. 2005; 366 (9499): 1809-1820.

3. Curtis MA, Zenobia C, Darveau RP. The relationship of the oral microbiotia to periodontal health and disease. Cell Host & Microbe. 2011; 10 (4): 302-306.

4. Kilian M, Chapple IL, Hannig M, Marsh PD, Meuric V, Pedersen AM et al. The oral microbiome-an update for oral healthcare professionals. Br Dent J. 2016; 221 (10): 657-666.

5. Van der Velden U. What exactly distinguishes aggressive from chronic periodontitis: is it mainly a difference in the degree of bacterial invasiveness? Periodontol 2000. 2017; 75 (1): 24-44.

6. Deng ZL, Sztajer H, Jarek M, Bhuju S, Wagner-Döbler I. Transcriptome profiles of key oral microbes in periodontal pocket compared to single laboratory culture reflect synergistic interactions. Front Microbiol. 2018; 9: 124.

7. López-Píriz R, Aguilar L, Giménez MJ. Management of odontogenic infection of pulpal and periodontal origin. Med Oral Patol Oral Cir Bucal. 2007; 12 (2): E154-E159.

8. Yoshimasu Y, Ikeda N, Sakai A, Yagi S, Hirayama Y, Morinaga S et al. Rapid bactericidal action of propolis against Porphyromonas gingivalis. J Dent Res. 2018; 97 (8): 928-300.

9. Hajishengallis G, Darveau RP, Curtis MA. The keystone-pathogen hypothesis. Nat Rev Microbiol. 2012; 10 (10): 717-725.

10. Listgarten MA, Wong MY, Lai CH. Detection of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Bacteroides forsythus in an A. actinomycetemcomitans-positive patient population. J Periodontol. 1995; 66 (2): 158-164.

11. Salavadhi SS, Chintalapani S, Ramachandran R, Nagubandi K, Ramisetti A, Boyapati R. Atomic force microscopy: a three-dimensional reconstructive tool of oral microbiota in gingivitis and periodontitis. J Indian Soc Periodontol. 2017; 21 (4): 264-269.

12. Chiranjeevi T, Prasad OH, Prasad UV, Kumar AK, Chakravarthi VP, Rao PB et al. Identification of microbial pathogens in periodontal disease and diabetic patients of south Indian population. Bioinformation. 2014; 10 (4): 241-245.

13. Greisen K, Loeffelholz M, Purohit A, Leong D. PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. J Clin Microbiol. 1994; 32 (2): 335-351.

14. Nobbs AH, Lamont RJ, Jenkinson HF. Streptococcus adherence and colonization. Microbiol Mol Rev. 2009; 73 (3): 407-450.

15. Haubek D, Ennibi OK, Poulsen K, Vaeth M, Poulsen S, Kilian M. Risk of aggressive periodontitis in adolescent carriers of the JP2 clone of Aggregatibacter (Actinobacillus) actinomycetemcomitans in Morocco: a prospective longitudinal cohort study. Lancet. 2008; 371 (96D8): 237-242.

16. Hajishengallis G. The inflammophilic character of the periodontitis-associated microbiota. Mol Oral Microbiol. 2014; 29 (6): 248-257.

17. Hajishengallis G. Immuno-microbial pathogenesis of periodontitis: keystones, pathobionts, and the host response. Trends Immunol. 2014; 35 (1): 3-11.

18. Stabholz A, Soskolne WA, Shapira L. Genetic and environmental risk factors for chronic periodontitis and aggressive periodontitis. Periodontol 2000. 2010; 53: 138-153.

19. Soga Y, Saito T, Nishimura F, Ishimaru F, Mineshiba J, Mineshiba F et al. Appearance of multidrug-resistant opportunistic bacteria on the gingiva during leukemia treatment. J Periodontol. 2008; 79 (1): 181-186.

20. Chen WP, Chang SH, Tang CY, Liou ML, Tsai SJ, Lin YL. Composition analysis and feature selection of the oral microbiota associated with periodontal disease. Biomed Res Int. 2018; 2018: 3130607.

21. Zhang Q, Qin XY, Jiang WP, Zheng H, Xu XL, Chen F. Comparison of subgingival and peri-implant microbiome in chronic periodontitis. Chin J Dent Res. 2015; 18 (3): 155-162.