medigraphic.com
Instituto Nacional de Neurología y Neurocirugía

2017, Número 4

<< Anterior Siguiente >>

Arch Neurocien 2017; 22 (4)


La tinción con el colorante rojo tiazina es un método de diagnóstico pos-mortem rápido y confiable para la enfermedad de Alzheimer

Castellanos-Aguilar L, Martínez-Zuñiga N, Gutierrez-Murcia JL, Viramontes-Pintos A, Garcés-Ramírez L, de la Cruz F, Guadarrama-Ortíz P, Luna-Muñoz J

Idioma: Español
Referencias bibliográficas: 28
Paginas: 33-44
Archivo PDF: 830.21 Kb.


RESUMEN

Hemos usado una variedad de marcadores inmunológicos combinados con el colorante rojo tiazina (RT), un marcador específico para diferenciar el estado fibrilar del no fibrilar del amiloide-β y tau en la enfermedad de Alzheimer. En este estudio hemos usado el RT como un marcador potencial de diagnóstico en la EA en tejido cerebral sin fijar en una impronta. En los experimentos control se incluia el uso de la tinción con el colorante tioflavina-S, tejido fijado y alguna doble tinción de este material con el RT. Se seleccionaron los marcadores para la proteína tau TaC-3 y 423. El analisis hecho indican que el RT mantiene una fuerte afinidad para las marañas neurofibrilares y placas neuríticas en corteza temporal de tejido sin fijar y congelado, este se descongelo previa la realización de la impronta. Esto indica que el TR es una herramienta potencial para un rápido diagnóstico posmortem de la neuropatología de la EA. La fijación no necesariamente es requerida para la inmunorreactividad de tau en los cortes histológicos.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. 1.Girych M, Gorbenko G, Maliyov I, Trusova V, Mizuguchi C, Saito H, et al. Combined thioflavin T-Congo red fluorescence assay for amyloid fibril detection. Methods Appl Fluoresc. 2016;4(3):034010. doi: 10.1088/2050- 6120/4/3/034010. PubMed PMID: 28355156.

  2. 2.Kumar A, Singh A, Ekavali. A review on Alzheimer's disease pathophysiology and its management: an update. Pharmacol Rep. 2015;67(2):195-203. doi: 10.1016/j.pharep.2014.09.004. PubMed PMID: 25712639.

  3. 3.Dubois B, Padovani A, Scheltens P, Rossi A, Dell'Agnello G. Timely diagnosis for Alzheimer's disease: a literature review on benefits and challenges. J Alzheimers Dis. 2016;49(3):617-31. doi: 10.3233/JAD-150692. PubMed PMID: 26484931; PubMed Central PMCID: PMCPMC4927869.

  4. 4.Braak H, Braak E. Neuropathological stageing of Alzheimer-related changes. Acta Neuropathol 1991;82(4):239- 59. PubMed PMID: 1759558.

  5. 5.Braak H, Braak E. Staging of Alzheimer's disease-related neurofibrillary changes. Neurobiol Aging 1995;16(3):271-8; discussion 8-84. PubMed PMID: 7566337.

  6. 6.McKhann G, Drachman D, Folstein M, Katzman R, Price D, Stadlan EM. Clinical diagnosis of Alzheimer's disease: report of the NINCDS-ADRDA work group under the auspices of department of Health and Human services task Force on Alzheimer's disease. Neurol 1984;34(7):939-44. PubMed PMID: 6610841.

  7. 7.Aboud O, Griffin WS. Silver staining of Alzheimer's disease. J Neurol Disord 2014;2(6). doi: 10.4172/2329- 6895.1000i103. PubMed PMID: 26949718; PubMed Central PMCID: PMCPMC4778739.

  8. 8.Gallyas F. Silver staining of Alzheimer's neurofibrillary changes by means of physical development. Acta Morphol Acad Sci Hung. 1971;19(1):1-8. PubMed PMID: 4107507.

  9. 9.Bussiere T, Bard F, Barbour R, Grajeda H, Guido T, Khan K, et al. Morphological characterization of thioflavin- S-positive amyloid plaques in transgenic Alzheimer mice and effect of passive Abeta immunotherapy on their clearance. Am J Pathol 2004;165(3):987-95. PubMed PMID: 15331422; PubMed Central PMCID: PMCPMC1618604.

  10. 10.Luna-Munoz J, Peralta-Ramirez J, Chavez-Macias L, Harrington CR, Wischik CM, Mena R. Thiazin red as a neuropathological tool for the rapid diagnosis of Alzheimer's disease in tissue imprints. Acta Neuropathol 2008;116(5):507-15. doi: 10.1007/s00401-008-0431-x. PubMed PMID: 18810470.

  11. 11.Moller HJ, Graeber MB. The case described by Alois Alzheimer in 1911. Historical and conceptual perspectives based on the clinical record and neurohistological sections. Eur Arch Psychiatry Clin Neurosci 1998;248(3):111-

  12. 12.Suenaga T, Hirano A, Llena JF, Ksiezak-Reding H, Yen SH, Dickson DW. Modified Bielschowsky and immunocytochemical studies on cerebellar plaques in Alzheimer's disease. J Neuropathol Exp Neurol 1990;49(1):31-40. PubMed PMID: 1688924.

  13. 13.Uchihara T. Silver diagnosis in neuropathology: principles, practice and revised interpretation. Acta Neuropathol 2007;113(5):483-99. PubMed PMID: 17401570.

  14. 14.Vallet PG, Guntern R, Hof PR, Golaz J, Delacourte A, Robakis NK, et al. A comparative study of histological and immunohistochemical methods for neurofibrillary tangles and senile plaques in Alzheimer's disease. Acta Neuropathol 1992;83(2):170-8. PubMed PMID: 1373017.

  15. 15.Kuninaka N, Kawaguchi M, Ogawa M, Sato A, Arima K, Murayama S, et al. Simplification of the modified Gallyas method. Neuropathol 2015;35(1):10-5. doi: 10.1111/neup.12144. PubMed PMID: 25178396; PubMed Central PMCID: PMCPMC4491351.

  16. 16.Tsamis K, Mytilinaios D, Njau SN, Psaroulis D, Mavroudis J, Costa V, et al. The combination of silver techniques for studying the pathology of Alzheimer's disease. Int J Neurosci 2008;118(2):257-66. doi: 10.1080/00207450701591115. PubMed PMID: 18205081.

  17. 17.Howie AJ, Owen-Casey MP. 'Apple-green birefringence' of amyloid stained by Congo red. Kidney Int. 2012;82(1):114; author reply doi: 10.1038/ki.2012.89. PubMed PMID: 22699383.

  18. 18.Khurana R, Uversky VN, Nielsen L, Fink AL. Is Congo red an amyloid-specific dye? J Biol Chem 2001;276(25):22715-21. doi: 10.1074/jbc.M011499200. PubMed PMID: 11410601.

  19. 19.Biancalana M, Koide S. Molecular mechanism of thioflavin-T binding to amyloid fibrils. Biochim Biophys Acta 2010;1804(7):1405-12. doi: 10.1016/j.bbapap.2010.04.001. PubMed PMID: 20399286; PubMed Central PMCID: PMCPMC2880406.

  20. 20.Santa-Maria I, Perez M, Hernandez F, Avila J, Moreno FJ. Characteristics of the binding of thioflavin S to tau paired helical filaments. J Alzheimers Dis 2006;9(3):279-85. PubMed PMID: 16914838.

  21. 21.Sun A, Nguyen XV, Bing G. Comparative analysis of an improved thioflavin-s stain, Gallyas silver stain, and immunohistochemistry for neurofibrillary tangle demonstration on the same sections. J Histochem Cytochem. 2002;50(4):463-72. PubMed PMID: 11897799.

  22. 22.Rajamohamedsait HB, Sigurdsson EM. Histological staining of amyloid and pre-amyloid peptides and proteins in mouse tissue. Methods Mol Biol 2012;849:411-24. doi: 10.1007/978-1-61779-551-0_28. PubMed PMID: 22528106; PubMed Central PMCID: PMCPMC3859432.

  23. 23.Matos LL, Trufelli DC, de Matos MG, da Silva Pinhal MA. Immunohistochemistry as an important tool in biomarkers detection and clinical practice. Biomark Insights 2010;5:9-20. PubMed PMID: 20212918; PubMed Central PMCID: PMCPMC2832341.

  24. 24.Resch JF, Lehr GS, Wischik CM. Design and Synthesis of a Potential Affinity Cleaving Reagent for Beta-Pleated Sheet Protein Structures. Bioorg Med Chem Lett 1991;1(10):519-22. doi: Doi 10.1016/S0960-894x(01)80457-1. PubMed PMID: WOS:A1991GN27800007.

  25. 25.Mena R, Edwards P, Perez-Olvera O, Wischik CM. Monitoring pathological assembly of tau and beta-amyloid proteins in Alzheimer's disease. Acta Neuropathol 1995;89(1):50-6. PubMed PMID: 7709731.

  26. 26.Mena R, Edwards PC, Harrington CR, Mukaetova-Ladinska EB, Wischik CM. Staging the pathological assembly of truncated tau protein into paired helical filaments in Alzheimer's disease. Acta Neuropathol 1996;91(6):633-

  27. 27.Luna-Munoz J, Chavez-Macias L, Garcia-Sierra F, Mena R. Earliest stages of tau conformational changes are related to the appearance of a sequence of specific phospho-dependent tau epitopes in Alzheimer's disease. J Alzheimers Dis 2007;12(4):365-75. PubMed PMID: 18198423.

  28. 28.Luna-Munoz J, Garcia-Sierra F, Falcon V, Menendez I, Chavez-Macias L, Mena R. Regional conformational change involving phosphorylation of tau protein at the Thr231, precedes the structural change detected by Alz- 50 antibody in Alzheimer's disease. J Alzheimers Dis 2005;8(1):29-41. PubMed PMID: 16155347.




2020     |     www.medigraphic.com