medigraphic.com
Federación Mexicana de Ginecología y Obstetricia, A.C.

2017, Número 07

<< Anterior Siguiente >>

Ginecol Obstet Mex 2017; 85 (07)


Alteración de los parámetros seminales y su asociación con la fragmentación del ADN espermático

Calull-Bagó A, González-Ortega C, Cancino-Villarreal P, Zúñiga-Sánchez P, Ruvalcaba-Ortega L, Gutiérrez-Gutiérrez AM

Idioma: Español
Referencias bibliográficas: 48
Paginas: 409-420
Archivo PDF: 295.89 Kb.


RESUMEN

Antecedentes: el patrón de referencia para evaluar la infertilidad masculina es el análisis seminal, aunque es incapaz de detectar la fragmentación del ADN espermático relacionado con resultados reproductivos adversos.
Objetivo: analizar la relación entre los parámetros seminales y la fragmentación del ADN espermático, y valorar la utilidad del seminograma como predictor de fragmentación.
Materiales y Métodos: estudio retrospectivo y observacional efectuado en pacientes del Instituto de Ciencias en Reproducción Humana Vida con problemas de fertilidad. Análisis seminal e índice de fragmentación del ADN (DFI) mediante el test de dispersión de la cromatina espermática.
Resultados: se estudiaron 206 pacientes: 43 (20.9%) con espermas normales y 163 (79.1%) con algún parámetro seminal alterado. En los individuos con espermas normales, 8 (18.6%) tuvieron fragmentación moderada y quienes resultaron con al menos un parámetro seminal alterado 49 (30.1%) reportaron fragmentación moderada y 22 (13.5%) fragmentación crítica. Se encontró una correlación negativa entre el índice de fragmentación del ADN y la movilidad, vitalidad y concentración (p‹0.0001). No se encontró correlación alguna con el resto de los parámetros seminales. Las medias de fragmentación aumentaron conforme se incrementó el número de parámetros alterados. La razón de momios de fragmentación moderada y crítica en pacientes con oligozoospermia fue de 2.2 y 1.9; con astenozoospermia 8.0 y 26.2; con necrozoospermia 10.5 y 18.8; y con ≥ 3 alteraciones seminales 4.6 y 14.2, respectivamente.
Conclusiones: en pacientes con astenozoospermia, necrozoospermia u oligozoospermia o con ≥ 3 parámetros seminales alterados se recomienda realizar el estudio de fragmentación del ADN.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. World Health Organization. WHO Laboratory Manual for the Examination and Processing of Human Semen, 5th ed. Switzerland: WHO press; 2010.

  2. Andrade-Rocha FT. Semen analysis in laboratory practice: an overview of routine tests. J Clin Lab Anal 2003;17(6):247-258.

  3. Agarwal A, Allamaneni SS. Sperm DNA damage assessment: a test whose time has come. Fertil Steril 2005;84(4):850- 853.

  4. Spano M, Seli E, Bizzaro D, Manicardi GC, Sakkas D. The significance of sperm nuclear DNA strand breaks on reproductive outcome. Curr Opin Obstet Gynecol 2005;17(3):255-260.

  5. Lin MH, Lee RKK, Li SH, Lu CH, Sun FJ, Hwu YM. Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic sperm injection, but might be related to spontaneous abortion rates. Fertil Steril 2008;90(2):352-359.

  6. Lewis SE, Agbaje I, Alvarez J. Sperm DNA tests as useful adjuncts to semen analysis. Syst Biol Reprod Med 2008;54(3):111-125.

  7. Vicari E, Perdichizzi A, De Palma A, Burrello N, D'Agata R, Calogero AE. Globozoospermia is associated with chromatin structure abnormalities: case report. Hum Reprod 2002;17(8):2128-2133.

  8. Brugnon F, Van Assche E, Verheyen G, Sion B, Boucher D, Pouly JL, Van Steirteghem A. Study of two markers of apoptosis and meiotic segregation in ejaculated sperm of chromosomal translocation carrier patients. Hum Reprod 2006:21(3):685-693.

  9. Simon L, Proutski I, Stevenson M, Jennings D, McManus J, Lutton D, Lewis SEM. Sperm DNA damage has a negative association with live-birth rates after IVF. Reprod Biomed Online 2013;26(1):68-78.

  10. Aitken RJ. Whither must spermatozoa wander? The future of laboratory seminology. Asian J Androl 2010;12(1):99-103.

  11. Zini A, Boman JM, Belzile E, Ciampi A. Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: systematic review and meta-analysis. Hum Reprod 2008;23(12):2663-2668.

  12. Benchaib M, Lornage J, Mazoyer C, Lejeune H, Salle B, Guerin JF. Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome. Fertil Steril 2007;87(1): 93-100.

  13. Muriel L, Garrido N, Fernández JL, Remohí J, Pellicer A, de los Santos MJ, Meseguer M. Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization and intracytoplasmic sperm injection. Fertil Steril 2006;85(2):371-383.

  14. Muriel L, Goyanes V, Segrelles E, Gosálvez J, Alvarez JG, Fernández JL. Increased aneuploidy rate in sperm with fragmented DNA as determined by the sperm chromatin dispersion (SCD) test and FISH analysis. J Androl 2007; 28(1):38-49.

  15. Carrell DT, Wilcox AL, Lowy L, Peterson CM, Jones KP, Erickson, et al. Elevated sperm chromosome aneuploidy and apoptosis in patients with unexplained recurrent pregnancy loss. Obstet Gynecol 2003;101(6):1229-1235.

  16. Sakkas D, Alvarez JG. Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis. Fertil Steril 2010;93(4):1027-1036.

  17. Xia Y, Cheng S, Bian Q, Xu L, Collins MD, Chang HC, Wang X. Genotoxic effects on spermatozoa of carbaryl-exposed workers. Toxicol Sci 2005;85(1):615-623.

  18. Younglai EV, Holt D, Brown P, Jurisicova A, Casper RF. Sperm swim-up techniques and DNA fragmentation. Hum Reprod 2001;16(9):1950-1953.

  19. Liu CH, Tsao HM, Cheng TC, Wu HM, Huang CC, Chen CI, Lee MS. DNA fragmentation, mitochondrial dysfunction and chromosomal aneuploidy in the spermatozoa of oligoasthenoteratozoospermic males. J Asist Reprod Genet 2004;21(4):119-126.

  20. Milazzo JP, Rives N, Mousset-Simeon N, Mace B. Chromosome constitution and apoptosis of immature germ cells present in sperm of two 47, XYY infertile males. Hum Reprod 2006;21(7):1749-1758.

  21. Mantas D, Angelopoulou R, Msaouel P, Plastira K. Evaluation of sperm chromatin quality and screening of Y chromosome microdeletions in Greek males with severe oligozoospermia. Arch Androl 2007;53(1):5-8.

  22. Perrin A, Caer E, Oliver-Bonet M, Navarro J, Benet J, Amice V, Morel F. DNA fragmentation and meiotic segregation in sperm of carriers of a chromosomal structural abnormality. Fertil Steril 2009;92(2):583-589.

  23. Fernández JL, Muriel L, Goyanes V, Segrelles E, Gosálvez J, Enciso M, De Jonge C. Halosperm® is an easy, available, and cost-effective alternative for determining sperm DNA fragmentation. Fertil Steril 2005;84(4):860.

  24. Evenson DP, Jost LK, Marshall D, Zinaman MJ, Clegg E, Purvis K, Claussen OP. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod 1999;14(4):1039-1049.

  25. Spanò M, Bonde JP, Hjøllund HI, Kolstad HA, Cordelli E, Leter G, Danish First Pregnancy Planner Study Team. Sperm chromatin damage impairs human fertility. Fertil Steril 2000;73(1):43-50.

  26. Evenson DP, Larson KL, Jost LK. Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. J Androl 2000;23(1):25-43.

  27. Evenson D, Wixon R. Meta-analysis of sperm DNA fragmentation using the sperm chromatin structure assay. Reprod Biomed Online 2006;12(4):466-472.

  28. Twigg JP, Irvine DS, Aitken RJ. Oxidative damage to DNA in human spermatozoa does not preclude pronucleus formation at intracytoplasmic sperm injection. Hum Reprod 1998;13(7):1864-1871.

  29. Ribas-Maynou J, GarcíaPeiró A, Fernández-Encinas A, Abad C, Amengual MJ, Prada, E, Benet J. Comprehensive analysis of sperm DNA fragmentation by five different assays: TUNEL assay, SCSA, SCD test and alkaline and neutral Comet assay. Andrology 2013;1(5):715-722.

  30. Godoy-Morales HS, Mamani-Cancino AD, Ponce-Barberena P, Lozano JM, González-Jara LC, Cedillo L, Mera R. Evaluación de los parámetros seminales en parejas con infertilidad. Rev Mex Reprod 2012; 5(1):8.

  31. Simon, L., Brunborg, G., Stevenson, M., Lutton, D., McManus, J., & Lewis, S. E. Clinical significance of sperm DNA damage in assisted reproduction outcome. Hum Reprod 2010;25(7):1594-1608.

  32. Rafighdoost, H., Farsi, M. M., Javadi, M., & Khafri, S. Relationship between Sperm Parameters and DNA Fragmentation using a Halosperm Kit. Anat Sci J 2013;10(2):79-86.

  33. Samplaski, M. K., Dimitromanolakis, A., Lo, K. C., Grober, E. D., Mullen, B., Garbens, A., & Jarvi, K. A. The relationship between sperm viability and DNA fragmentation rates. Reprod Biol Endocrinol 2015;13(1):42.

  34. Boushaba, S., & Belaaloui, G. Sperm DNA fragmentation and standard semen parameters in Algerian infertile male partners. World J Mens Health 2015;33(1):1-7.

  35. Aydos, O. S., Yükselten, Y., Kaplan, F., Sunguroğlu, A., & Aydos, K. Analysis of the correlation between sperm DNA integrity and conventional semen parameters in infertile men. Turk J Urol 2015;41(4):191.

  36. Sheikh, N., Amiri, I., Farimani, M., Najafi, R., & Hadeie, J. Correlation between sperm parameters and sperm DNA fragmentation in fertile and infertile men. Iran J Reprod Med 2008;6(1):13-18.

  37. Wyrobek, A. J., Eskenazi, B., Young, S., Arnheim, N., Tiemann-Boege, I., Jabs, E. W. & Evenson, D. Advancing age has differential effects on DNA damage, chromatin integrity, gene mutations, and aneuploidies in sperm. Proc Natl Acad Sci U S A 2006;103(25):9601-9606.

  38. Aitken, R. J., Clarkson, J. S., & Fishel, S. Generation of reactive oxygen species, lipid peroxidation, and human sperm function. Biol Reprod 1989;41(1):183-197.

  39. Aitken, R. J., Clarkson, J. S., Hargreave, T. B., Irvine, D. S., & Wu, F. C. Analysis of the relationship between defective sperm function and the generation of reactive oxygen species in cases of oligozoospermia. J Androl 1989;10(3):214-220.

  40. Aitken, R. J., Irvine, D. S., & Wu, F. C. Prospective analysis of sperm-oocyte fusion and reactive oxygen species generation as criteria for the diagnosis of infertility. Am J Obstret Gynecol 1991;164(2):542-551.

  41. Sun, J. G., Jurisicova, A., & Casper, R. F. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol Reprod 1997;56(3):602-607.

  42. Yılmaz, S., Zergeroğlu, A. D., Yılmaz, E., Sofuoglu, K., Delikara, N., & Kutlu, P. Effects of sperm DNA fragmentation on semen parameters and ICSI outcome determined by an improved SCD test, Halosperm. Cell j (Yakhteh) 2010;4(2).

  43. Erenpreiss, J., Spano, M., Erenpreisa, J., Bungum, M., & Giwercman, A. Sperm chromatin structure and male fertility: biological and clinical aspects. Asian J Androl 2006;8(1):11-29.

  44. Irvine, D. S., Macleod, I. C., Templeton, A. A., Masterton, A., & Taylor, A.. Andrology: A prospective clinical study of the relationship between the computer-assisted assessment of human semen quality and the achievement of pregnancy in vivo. Hum Reprod 1994;9(12):2324-2334.

  45. Brahem, S., Jellad, S., Ibala, S., Saad, A., & Mehdi, M. DNA fragmentation status in patients with necrozoospermia. Syst Biol Reprod Med 2012;58(6):319-323.

  46. Moustafa, M. H., Sharma, R. K., Thornton, J., Mascha, E., Abdel-Hafez, M. A., Thomas, A. J., & Agarwal, A. Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility. Hum Reprod 2004;19(1):129-138.

  47. Shen, H. M., Dai, J., Chia, S. E., Lim, A., & Ong, C. N. Detection of apoptotic alterations in sperm in subfertile patients and their correlations with sperm quality. Hum Reprod 2002;17(5):1266-1273.

  48. Virro, M. R., Larson-Cook, K. L., & Evenson, D. P. Sperm chromatin structure assay (SCSA®) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles. Fertil Steril 2004;81(5):1289- 1295.




2020     |     www.medigraphic.com