Administración neonatal única de aceite de soya y/o tamoxifeno afecta permanentemente a la histomorfología testicular en ratas adultas

Alicia González-González; Everardo González-Padilla; Francisco Fierro-Fierro; María de Lourdes Juárez-Mosqueda; Juan José Pérez-Rivero; Marcela Vergara-Onofre

2016, Número 2

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Vet Mex 2016; 3 (2)

Administración neonatal única de aceite de soya y/o tamoxifeno afecta permanentemente a la histomorfología testicular en ratas adultas

González-González A, González-Padilla E, Fierro-Fierro F, Juárez-Mosqueda ML, Pérez-Rivero JJ, Vergara-Onofre M

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Idioma: Español/Inglés
Referencias bibliográficas: 50
Paginas: 1-13
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RESUMEN

El objetivo de este estudio fue evaluar el efecto de la administración de tamoxifeno (Tx) y aceite de soya (SO) durante el periodo crítico de la diferenciación sexual hipotalámica en ratas macho recién nacidas, en el contexto de la histomorfología gonadal durante la edad adulta. Los animales se dividieron al azar en tres grupos (n = 5 cada uno). Una hora después del nacimiento, un grupo fue tratado por vía subcutánea con 200 µg de Tx y 20 µL de SO comercial como vehículo; mientras otro grupo fue tratado solo con 20 µL de SO, y el grupo control no recibió ningún tratamiento. Todas las ratas se pesaron y sacrificaron por dislocación cervical a los 90 días postratamiento. Se extrajeron los testículos, se pesaron y procesaron para la evaluación histológica. La administración única de Tx y de SO durante el periodo crítico de la diferenciación sexual del hipotálamo altera de forma permanente la histomorfología testicular, la espermatogénesis y el peso corporal en la edad adulta. Las alteraciones incluyen: vacuolización, reducción del número de espermatogonias y de células de Sertoli. La administración de Tx redujo el peso testicular, el diámetro y el área de los túbulos seminíferos, así como la altura del epitelio germinal, e incrementó el espacio intertubular. El SO redujo el número de espermatocitos y espermátides, incluso más que el Tx. No se afectó el número de células de Leydig. La posibilidad de que el aceite de soya actúe como disruptor endócrino, amerita más atención y abre la posibilidad para el desarrollo de nuevos métodos de control de plagas.

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