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2015, Número 2

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Gac Med Mex 2015; 151 (2)


Caracterización de un modelo hemodinámico ex vivo para el estudio de la activación endotelial por el factor de necrosis tumoral α (TNF-α) en venas humanas perfundidas

López-Macay A, Ruiz-Medina EJ, Ventura-Gallegos JL, Arechavaleta-Velasco F, Gómez-Quiroz LE, Konigsberg-Fainstein M, Zentella-Dehesa A

Idioma: Español
Referencias bibliográficas: 38
Paginas: 206-215
Archivo PDF: 318.83 Kb.


RESUMEN

Los procesos inflamatorios son reconocidos como parte de la etiología de una gran cantidad de enfermedades cronicodegenerativas. La interacción entre las células del sistema inmunológico y las locales y moléculas como citosinas y quimiocinas permite la activación celular y la amplificación de la respuesta. Actualmente se reconoce la importancia de los factores fisicoquímicos como el flujo vascular, las fuerzas de rozamiento y la presión durante este evento, puesto que modulan la expresión génica y la activación endotelial, pero existen pocos estudios que recreen dicho microambiente celular. Por ello, surge la necesidad de diseñar nuevos modelos que simulen condiciones cercanas a las fisiológicas. Nuestro objetivo fue establecer un modelo ex vivo de vena humana que permitiera activar al endotelio en condiciones de flujo, para estudiar los componentes moleculares de la adhesión, considerando parámetros fisicoquímicos como el flujo y el rozamiento. Se empleó la vena endotelial umbilical humana, que se activó con el TNF-α, para determinar la adhesión de células monocíticas U937 circulantes, así como la secreción de citocinas y la expresión de la molécula de adhesión ICAM-1. Este modelo permitirá estudiar la adhesión leucocitaria en condiciones de flujo empleando distintos estímulos inflamatorios, así como las vías de señalización implicadas en diversas enfermedades.


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