Reyes SM, Stuardo RJA, Barros RC

Idioma: Español/Inglés
Referencias bibliográficas: 27
Paginas: 389-395
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RESUMEN

Este trabajo comparó el efecto de tres medios de cultivo sobre el desarrollo embrionario de ovocitos bovinos fecundados in vitro. Se utilizaron tres medios: a) Medio testigo (TCM – 199 + 10 mg/ml), b) medio testigo con células epiteliales del oviducto bovino (BOEC) y c) medio testigo más 5 mg/ml insulina, 5 mg/ml transferrina y 5 mg/ml selenio (ITS). Los ovocitos fecundados in vitro se incubaron separadamente en cada uno de los medios de cultivo durante seis días. Los resultados mostraron que después de 24 h de incubación los porcentajes de desarrollo al estado de dos células fueron de 44.9 44.1 y 43, respectivamente (P › 0.05). Sin embargo, el desarrollo hasta el estado blastocisto temprano (seis días de cultivo) fue mayor (P ‹ 0.05) en medio TCM – 199 + ITS (20.3%) comparado con los otros dos medios utilizados (12.3% y 1.7%) en TCM + BOEC y testigo, respectivamente.

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