2005, Número 4
Clonación, secuenciación y expresión del gen prn de pertactina de Bordetella bronchiseptica de origen canino
Basurto-Alcántara FJ, Hernández-Castro R, Verdugo-Rodríguez A, Rosales ME, Montaraz CJA
Idioma: Español/Inglés
Referencias bibliográficas: 31
Paginas: 425-436
Archivo PDF: 566.12 Kb.
RESUMEN
La pertactina (Prn) es una proteína de la membrana externa de
Bordetella bronchiseptica con propiedades de adhesina y de autotransportador del sistema de secreción tipo V, que posee dos regiones variables en la estructura primaria de la proteína y que induce inmunidad protectora en cerdos y ratones. En este trabajo, el gen
prn fue clonado, secuenciado y expresado en
E. coli a partir de un aislamiento de campo, cepa P98 de
Bordetella bronchiseptica de origen canino. La detección de Prn fue realizada con el empleo de los anticuerpos contra
Bordetella, mediante Inmunoblot. Con el análisis de la secuencia de aminoácidos se identificó el dominio RGD de adherencia a células epiteliales en los aminoácidos 261 al 263 y las dos regiones de repeticiones variables ubicadas entre el aminoácido Gly 264 a la Pro 278 que corresponde a la región I y de la Pro 568 a la Pro 587 para la región II.
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