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2013, Número 3

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Biotecnol Apl 2013; 30 (3)


Caracterización molecular y bioquímica de plantas de tomate (Solanum lycopersicum L.) cv. Micro-Tom sometidas a estrés inducido por plomo (Pb)

Pérez S, Ahmed AIS, Cabezas D

Idioma: Ingles.
Referencias bibliográficas: 53
Paginas: 194-198
Archivo PDF: 196.53 Kb.


RESUMEN

Se estudió la respuesta de plantas de tomate Solanum lycopersicum L. cv. Micro-Tom (MT) sometidas a estrés inducido por plomo (5 y 10 mg/kg de PbAc2) y en las fases de desarrollo vegetativo germinación-floración y floración-fructificación. Se determinaron los niveles de expresión en estas condiciones de los genes inducidos por metales pesados superóxido dismutasa (SOD; EC 1.15.1.1) e isoflavona reductasa (IFR; EC 1.6.4.2), y otro de potencial interés en este campo (proteína tumoral controlada durante la transcripción-TCTP). En la primera fase, la TCTP fue reprimida en el grupo control y a la máxima concentración empleada de PbAc2, la expresión de la SOD fue baja en las dos concentraciones y la IFR fue mayor a 10 mg/kg. En la segunda fase, los tres genes se expresaron en las dos concentraciones, la TCTP y la SOD a mayores niveles en 5 mg/kg de PbAc2. Se determinó por densidad óptica la actividad específica de SOD y de la glutatión reductasa (GR; EC 1.6.4.2), junto con la concentración de proteínas totales. Ambas enzimas antioxidantes mostraron mayor actividad específica a 10 mg/kg de PbAc2, con diferencias estadísticamente significativas con respecto al tratamiento control, y la GR también mostró diferencias estadísticamente significativas con respecto al tratamiento con 5 mg/kg. La concentración de proteínas totales fue mayor en el grupo control, y estadísticamente significativa con respecto a la del tratamiento con 10 mg/kg. Esta investigación sugiere que la toxicidad por plomo provoca la inducción de enzimas antioxidantes claves del sistema de defensa en las plantas de tomate.


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