Reytor ML, González Y, Pascual I, Hernández A, Chávez MA, Alonso DRM

Idioma: Ingles.
Referencias bibliográficas: 38
Paginas: 77-82
Archivo PDF: 121.28 Kb.

RESUMEN

Una de las estrategias más promisorias para contarrestar la malaria es controlar la infectividad del parásito Plasmodium, con fármacos dirigidos hacia sus blancos principales. Se ha demostrado que proteasas (pertenecientes a diferentes clases mecanísticas) participan en el mecanismo de infección, por lo que se han convertido en blancos promisorios para el tratamiento de la enfermedad. En este trabajo se investigaron extractos crudos y clarificados de cinco especies de ascidias cubanas en busca de actividad inhibidora de proteasas pertenecientes a diferentes clases mecanísticas. Las ascidias son animales invertebrados que presentan numerosas moléculas bioactivas, aunque solo unos pocos inhibidores de proteasas han sido aislados a partir de ellas. En esta investigación se informa por primera vez la presencia de actividad inhibidora de carboxipeptidasa A y B pancreática bovina, aminopeptidasa N de riñón porcino y subtilisina A de Bacillus licheniformis en algunos de los extractos de ascidias evaluados. Los extractos más promisorios se caracterizaron con relación al comportamiento de la concentración de extracto en función de la actividad enzimática residual, la determinación de los valores de IC50 y actividad inhibidora específica (SIA), y la evaluación de su actividad proteolítica a través de ensayos zimográficos. Investigaciones actuales se encuentran dirigidas a la purificación y caracterización de estas moléculas.

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