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2012, Número 1

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Biotecnol Apl 2012; 29 (1)


Inducción de quitinasas y glucanasas en cepas de Trichoderma spp. promisorias como agentes para el control biológico

González I, Infante D, Martínez B, Arias Y, González N, Miranda I, Peteira B

Idioma: Ingles.
Referencias bibliográficas: 32
Paginas: 12-16
Archivo PDF: 158.86 Kb.


RESUMEN

La secreción de enzimas hidrolíticas es uno de los mecanismos de los hongos del género Trichoderma, para ejercer el control biológico de plagas y enfermedades. Las quitinasas y glucanasas son dos de estas enzimas que degradan la pared celular de los hongos fitopatógenos y la cutícula de insectos y nematodos. Se evaluó la dinámica de inducción de esas enzimas en diez cepas de Trichoderma spp. en tres medios líquidos con inductores diferentes: medio basal, medio basal suplementado con quitina al 0.5% y medio basal suplementado con gelatina al 0.2%. Las actividades quitinasas y glucanasas se evaluaron al primer, tercer, quinto y séptimo día del cultivo. Los mayores valores de la actividad quitinasa se alcanzaron en los medios basal y basal suplementado con quitina, mientras que los medios basal y basal suplementado con gelatina resultaron mejores inductores de las β-1.3-glucanasas.


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