Reséndiz M, Montalvo-Corral M, Flores-Mendoza L, Ramírez-Mendoza H, Segalés J, Hernández J

Idioma: Español/Inglés
Referencias bibliográficas: 30
Paginas: 45-58
Archivo PDF: 447.75 Kb.

RESUMEN

Los objetivos de este trabajo fueron la cuantificación de circovirus porcino tipo 2 (PCV2) de virus del síndrome respiratorio y reproductivo porcino (PRRSV), así como la expresión de citocinas (IL-6, IL-12, TNF-α, e IFN-γ) en nódulos linfáticos inguinales superficiales (NLIS) de cerdos afectados por el síndrome de desmedro posdestete (postweaning multisystemic wasting syndrome, PMWS) y en cerdos sanos. Con base en el diagnóstico clínico y a la cuantificación de PCV2 por hibridación in situ y qRT-PCR, los cerdos se distribuyeron en tres grupos: 1) PMWS, cerdos con signos de desmedro y con cargas virales altas en NLIS (n = 4); 2) con desmedro sin PMWS, cerdos con signos de desmedro y cargas virales de intermedias a bajas en NLIS (n = 3); y 3) cerdos sanos, sin signos clínicos y con cargas virales bajas (n = 3). El PRRSV fue detectado en tres de los cerdos afectados por el PMWS y en dos cerdos con desmedro, pero sin el síndrome. Se caracterizaron las secuencias nucleotídicas del ORF2 de los PCV2 encontrados y los análisis genéticos revelaron la presencia de 2 genotipos PCV2a y PCV2b en las granjas afectadas con el PMWS. El perfil de expresión de citocinas mostró una baja expresión de IFN-γ en los cerdos con PMWS, mientras que los cerdos con desmedro sin PMWS mostraron valores elevados de esta citocina. Estos resultados sugieren que existe un desbalance en la producción de citocinas que puede estar implicado en la patogénesis de la enfermedad.

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