García-Contreras R, Scougall-Vilchis RJ, Contreras-Bulnes R, Adachi K, Sakagami H, Hibino Y, Nakajima H, Shimada J

Idioma: Español
Referencias bibliográficas: 34
Paginas: 175-182
Archivo PDF: 218.65 Kb.

RESUMEN

Objetivo: Investigar los efectos de la irradiación ultravioleta (UV) sobre placas de titanio (Ti) para la adhesión osteoblástica por medio de un método colorimétrico simple y reproducible para determinar la densidad celular.
Materiales y métodos: Dos diferentes tamaños (10x10x0.5 mm y 20x20x0.05 mm) de placas (n=10 c/gp) fueron obtenidos de una hoja de Ti puro y divididas en dos grupos (n=5/gp) para cada tamaño de placa. La superficie de las placas de Ti fue pulida, observada con microscopía electrónica de barrido (MEB) y estimada la rugosidad de la superficie pulida. Para el grupo experimental, las placas de Ti fueron irradiadas a una longitud de onda de 253.7 nm con luz UV durante 5, 20, 40, 60 minutos ó 4 y 6 horas. Células osteoblásticas de ratón MC3T3-E1 fueron cultivadas en medio alfa mínimo esencial (α-MEM) e inoculadas sobre cada placa de Ti. El número de células adheridas y proliferadas fue determinado por medio del método de MTT y el consumo de aminoácidos.
Resultados: 20 minutos de irradiación UV de las placas de Ti fue suficiente para incrementar significativamente (p‹0.05) la adhesión y proliferación celular, acompañado de un mayor consumo de aminoácidos.
Conclusiones: La irradiación UV sobre placas de Ti incrementó significativamente la adhesión celular por medio del método de MTT, confirmando la potencialidad de la luz UV.

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