medigraphic.com

1994, Número 3

<< Anterior Siguiente >>

Vet Mex 1994; 25 (3)


Aspectos fisiológicos acerca del congelamiento de semen

Palacios AA

Idioma: Español/Inglés
Referencias bibliográficas: 40
Paginas: 207-210
Archivo PDF: 414.02 Kb.


FRAGMENTO

Siendo el agua la fuente esencial para las funciones vitales de cualquier organismo, no es raro pensar que la falta o solidificación de ésta cause incompatibilidad para la ida. Sin embargo, la permanencia de una célula en estado de congelación se contrapone a lo anteriormente dicho, ya que con el método de congelamiento se puede preservar una célula a temperaturas extremadamente bajas, permitiendo que el metabolismo se reduzca "absolutamente", sin que pierda su potencial vital. A temperaturas de -196°C no hay reacciones bioquímicas ni energía térmica dentro de la célula, aún más, no hay evidencia de que puedan haber cambios de índole genético. No obstante, dicha estabilidad solamente puede ser mantenida a temperaturas por debajo de los 130°C, ya que, a temperaturas mayores, puede haber agua no congelada intracelularmente la cual permite funciones metabólicas, causando degradación de la célula.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. Abdelhakeam, A.A., Graham, E.F. and Vazquez, I.A.: Studies on the presence and absence of glycerol in unfrozen and frozen ram semen: Fertility trials and the effect of dilution methods on freezing ram semen in the absence of glycerol. Cryobiology, 28: 36-42 (1991).

  2. Amann, R.P. and Pickett, B.W.: Principles of cryopreservation and a review of cryopreservation of stallion spermatozoa. Equine vet. Sci., 7: 145-173 (1987).

  3. Arns, M.J., Webb, G.W., Kreider, J.L., Potter, G.D. and Evans, J.W.: Use of different nonglycosable sugars to mantain stallion sperm viability when frozen or stored at 37°C and 5°C in a bovine serum albumin medium. J. Reprod. Fertil., 35 (Suppl.): 135-141 (1987).

  4. Beall, P.T.: States of water in biological systems. Cryobiology., 20: 324-334 (1983).

  5. Bustamante, G.C.: Acción del sulfóxido de dimetil y glicerol como agentes crioprotectores del acrosoma del espermatozoide de carnero durante la congelación. Tesis de maestría. Fac. de Med. Vet. y Zoot. Universidad Nacional Autónoma de México. México, D.F., 1980.

  6. Clay, C.M., Slade, N.P., Amann, R.P. and Squires, E.L.: Effect of dilution, polyvinil alcohol (PVA) and bovine serum albumin (BSA) on stallion spermatoza motility. Proceedings of the 10th International Congress of Animal Reproduction and Artificial Insemination. Urbana, Illinois 1984. 167-169. Animal Reproduction and Artificial Insemination Association. Urbana, Illinois (1984 b).

  7. Cochran, J.D., Amann, R.P., Froman, D.P. and Pickett, B.W.: Effects of centrifugation, glycerol level, cooling to 5°C, freezing rate and thawing rate on the post-thaw motility of equine sperm. Theriogenology, 22: 25-34 (1984).

  8. Coulter, G.H. and Foote, R.H.: Lipid deficient extender for bovine spermatozoa: Its development and use in measuring freezing-induced lipid loss. J. Dairy Sci., 58: 82-87 (1975).

  9. Cristanelli, M.J., Amann, R.P., Squires, E.L. and Pickett, B.W.: Effects of egg yolk and glycerol levels in lactose-egg yolk extender and on freezing rate on the motility of frozen-thawed stallion spermatozoa. Theriogenology, 24: 681-686 (1985).

  10. Daw, A., Farrant, J. and Morris, G.J.: Membrane leakage of solutes after thermal shock or freezing. Cryobiology, 10: 126-133 (1973).

  11. Fahy, G.M.: The relevance of cryoprotectant "toxicity" to cryobiology. Cryobiology, 23: 1-13 (1986).

  12. Franks, F., Mathias, S.F., Galfre, P., Webster, S.D. and Brown, D.: Ice nucleation and freezing in undercooled cells. Cryobiology, 20: 298-309 (1983).

  13. Gómez, J.A.: The use of bovine blood serum as an extender for bull semen. M.Sc. thesis. Dep. Anim. Sci. Texas A&M University. College Station, Texas, 1968.

  14. Guay, P., Rondeau, M. and Boucher, S.: Effect of glycerol on motility, viability, aspartate aminotransferase release and of stallion semen before and after thawing. Equine vet. J., 13: 177-182 (1981).

  15. Heiskanen, M., Pirhonen, A., Koskinen, E. and Mãenpãã, P.H.: Motility and ATP content of extended equine spermatozoa in different storage conditions. J. Reprod. Fertil., 35 (Suppl.): 103-107 (1987).

  16. Jasko, D.J.: Evaluation of stallion semen. Memorias del Curso Internacional de Reproducción Equina. Fac. de Med. Vet. y Zoot. Universidad Nacional Autónoma de México. México, D.F. 1993. 99-118. Fue. de Med. Vet. y Zoot. Universidad Nacional Autónoma de México. México, D.F. (1993).

  17. Jasko, D.J., Moran, D.M.,Farlin, M.E., Squires, E.L., Amann, R.P. and Pickett, B.W.: Pregnancy rates utilizing fresh, cooled and frozen-thawed stallion semen. Memorias del Curso Internacional de Reproducción Equina. Fac. de Med. Vet. y Zoot. Universidad Nacional Autónoma de México. México, D.F. 1993. 77-98. Fac. de Med. Vet. y Zoot. Universidad Nacional Autónoma de México. México, D.F. (1993).

  18. Jiménez, D.A., Chandler, J.E., Adkinson, R.W., Barta, O., Ingraham, R.H. and Saxton, A.: Effect of serum sources and colostral whey on bovine semen quality and spermatozoa immunoglobulin G immunofluorescence. J. Dairy Sci., 69: 2704-2710 (1986).

  19. Jones, R.: The use of dimethyl sulfoxide, glycerol and reconstituted skim milk on the preservation of ram spermatozoa. Aust. J. Biol. Sci., 18: 887-900 (1965).

  20. Klem, M.E., Kreider, J.L. and Potter, G.D.: Influence of blood protein on the motility and viability of equine spermatozoa. Proceedings of the Horse Short Course. Dep. Anim. Sci. Texas A&M University. College Station,Texas. 1984. 37-38. Texas A&M University. College Station, Texas (1984).

  21. Koskinen, E., Junnila, M., Katila, T. and Soini, H.: A preliminary study on the use of betaine as a cryoprotective agent in deep freezing of stallion semen. J. vet. Med., 36: 110-114 (1989).

  22. Kreider, J.L., Tindall, W.C. and Potter, G.D.: Inclusion of bovine serum albumin in semen extenders to enhance maintenance of stallion sperm viability. Theriogenology, 23: 399-408 (1985).

  23. Lehninger, A.L.: Bioquímica. 2a ed. Omega, Barcelona, España, 1985.

  24. Levine, R.J., Ravi, M.M., Brown, M.H., Hurtt, M.E., Bentley, K.S. and Moho, K.L.: Computer assisted semen analysis: Results vary across technicians who prepare videotapes. Fertil. Steril., 52: 673-677 (1989).

  25. Mazur, P.: Freezing of living cells: Mechanisms and implications. Am. J. Physiol., 247: 125-142 (1984).

  26. Montes, R.C.: Comparación de tres fuentes biológicas para producir anticuerpos contra progesterona para utilizarse en radioinmunoanálisis o enzimoinmunoanálisis. Tesis de doctorado. Fac. de Med. Vet. y Zoot. Universidad Nacional Autónoma de México. México, D.F., 1991.

  27. Mortimer, D., Serres, C., Mortimer, S.T. and Jouannet, P.: Influence of image sampling frequency on the perceived movement characteristics of progressively motile human spermatozoa. Gamete Res., 20: 313-327 (1988).

  28. Palacios, A., Valencia, J. y Zarco, L.: Efecto del sistema de envasado y la concentración espermática sobre el daño acrosomal y la motilidad posdescongelación del semen de equino. Vet. Méx., 23: 315-318 (1992).

  29. Parks. J.E. and Graham, J.K.: Effects of cryopreservation procedures on sperm membranes. Theriogenology, 38: 209-222 (1992).

  30. Pedigo, N.G., Vernon, M.W. and Curry, T.E.: Characterization of a computerized semen analysis system. Fertil. Stetil., 52: 659-666 (1989).

  31. Phillips, P.H. and Lardy, H.A.: A yolk-buffer pabulum for the preservation of bull semen. J. Dairy Sci., 23: 399-404 (1940).

  32. Pontbriand, D., Howard, J.G., Schiewe, M.C., Stuart, L.D. and Wildt, D.E.: Effect of cryoprotective diluent and method of freeze-thawing on survival and acrosomal integrity of ram spermatozoa. Cryobiology, 26: 341-354 (1989).

  33. Province, C.A., Squires, E.L., Pickett, B.W. and Amann, R.P.: Cooling rates, storage temperatures and fertility of extended equine spermatozoa. Theriogenology, 23: 925-934 (1985).

  34. Pruitt, J.B.: Fertility of stallion semen extended with a sucrose- BSA extender, M.Sc. thesis. Dep. Anim. Sci. Texas A&M University. College Station, Texas, 1985.

  35. Reid, J.L.: An application of a bovine serum albumin extender for stallion semen. M. Agriculture thesis. College of Agriculture. Texas A&M University. College Station, Texas, 1986.

  36. Rudolph, A.S., Crowe, J.I., Spargo, B. and Gowe, L.M.: Interaction of cryoprotectants with lipid bilayers Cryobiology, 23: 543 (1986) (Abstract).

  37. Salisbury, G.W., Fullers, H.K. and Willett, E.L.: Preservation of bovine spermatozoa in yolk-citrate diluent and field results from its use. J. Dairy Sci., 24: 910 (1941).

  38. Senger, P.L., McCutchan, J.F. and Hillers, J.K.: Influence of blood serum from bulls and heifers on head-to-head agglutination and acrosomal maintenance in bovine spermatozoa. Biol. Reprod. 25: 433-437 (1981) (Abstract).

  39. Watson, P.F.: The interaction of egg yolk and ram spermatozoa studied with a fluorescent probe. J. Reprod. Fertil., 42: 105-111 (1975).

  40. Watson, P.F.: Problems in the cryopreservation of mammalian spermatozoa. Cryobiology, 23: 547 (1986) (Abstract).




2020     |     www.medigraphic.com