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2009, Número 1

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Vet Mex 2009; 40 (1)


Efecto del virus del síndrome reproductivo y respiratorio porcino (PRRS) en células dendríticas de cerdo derivadas de monocitos

Flores-Mendoza L, Silva-Campa E, Reséndiz M, Mata-Haro V, Osorio FA, Hernández J

Idioma: Español/Inglés
Referencias bibliográficas: 48
Paginas: 39-54
Archivo PDF: 547.62 Kb.


RESUMEN

Las células dendríticas (DC) son las presentadoras de antígeno más importantes del sistema inmune. Su localización anatómica (piel, mucosas y sangre periférica), la expresión de receptores para reconocer patógenos, la expresión de moléculas de coestimulación (CD80/86), del complejo principal de histocompatibilidad (MHC) clases I y II, y la producción de citocinas (IFN-α, IL-10, IL-12), les confiere una característica única para regular las respuestas inmune innata y adaptativa. El objetivo de este trabajo fue evaluar el efecto del virus de síndrome reproductivo y respiratorio porcino (PRRS) en DC maduras. Se generaron células dendríticas a partir de monocitos utilizando IL-4 y GM-CSF y se estimularon con lipopolisacárido (LPS) para inducir su maduración. Los resultados muestran que la expresión de las moléculas CD14 y CD172a no se altera en las DC infectadas, mientras que la expresión de MHC II y CD80/86 se ve disminuida. Esta disminución parece afectar la proliferación alogénica de linfocitos estimulados con DC infectadas. Asimismo, el virus aumenta la expresión del ARNm de IL-10 y TNF-α, y disminuye la de IL-1β e IL-6. Lo anterior explica, en parte, la inmunopatología de la enfermedad.


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