a. Are essential for the treatment, prevention, diagnosis or study of the etiology of any disease

b. The experimental results cannot be obtained by means of other procedures

c. The results are not known in advance, nor is it possible to predict them

2. The animals selected for an investigation must be of the species, age and characteristics suitable for the type of study to be carried out, as well as the minimum number of animals necessary to obtain result

3. All animals destined for research must be legally acquired and come from accredited animal facilities

4. The animals must be housed in clean, dry facilities, adequately designed so that they are comfortable, with free access to water and food

5. The conditions that are offered to the animals should promote their health

6. Researchers must treat animals as beings that are capable of feeling pain and anguish

7. Any procedure that causes pain or anguish must be performed under analgesia or anesthesia

8. The administration of anesthetics and tranquilizers to the animals during the experiments must be supervised by a veterinarian

9. No painful or invasive procedure should begin until it is certain that the animal is under anesthesia

10. Researchers who work with animals must be aware that when they take the life of an animal into their hands, they also have the responsibility of ensuring a good death, providing euthanasia when necessary

11. At the end of the experiment, the animal must receive a dignified death, fast and with a minimum of pain