2020, Number 4
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Rev Mex Patol Clin Med Lab 2020; 67 (4)
Distribution of Ct values for SARS-CoV-2 RT-PCR at of diagnosis in Mexican pediatric patients
Parra-Ortega I, Carbajal-Franco E, Vilchis-Ordoñez A, Ángeles-Floriano T, Nieto-Rivera B, López-Martínez I, Barrera-Badillo G, Klunder-Klunder M, López-Martínez B
Language: Spanish
References: 31
Page: 176-182
PDF size: 251.14 Kb.
ABSTRACT
Introduction: The information generated by the clinical laboratory to support the diagnosis of infection by the SARS-CoV-2 virus based on molecular tests, which supports the care of patients, the behavior of the results of diagnostic tests in pediatric patients has some characteristics important to highlight.
Material and methods: From March to July of 2020, 1,325 nasopharyngeal swab samples from pediatric patients were processed to identify the SARS-CoV-2 virus. In all cases, the values of the cycle threshold (Ct) of the viral genes and the internal control were recorded. In this study, the relative viral quantification was estimated only by the Ct value of ORF1ab gene.
Results: 176 (13.28%) positive results were identified, 103/176 (58.5%) were male aged 0-17 years, with a mean of 6 years 5 months and a median of 5 years 3 months, while 73/176 (41.5%) of the female sex, with an age range of 0-18 years with a mean of 6.2 years and a median of 4 years 5 months. The distribution of the Ct values. Values obtained by age group, a median of 31.9 to 34.01 was observed, and when comparing the distribution versus adult patients, no differences were observed.
Conclusion: Ct values at the time of diagnosis, identified in Mexican children, are higher than those reported in other parts of the world, which could suggest a lower amount of viral RNA in our population at the time of diagnosis.
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