Immunology   Evaluation of redox state and some immnunological markers in seropositive individuals to the linfotropic virus of human T cells type I

Lizette Gil; Yamina Daumy; Liliana Pérez; Alejandro Alvarez; Héctor Díaz; Caridad Luzardo; Rolando Tápanes; Jorge Pérez

2002, Number 2

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Bioquimia 2002; 27 (2)

Immunology
Evaluation of redox state and some immnunological markers in seropositive individuals to the linfotropic virus of human T cells type I

Gil L, Daumy Y, Pérez L, Alvarez A, Díaz H, Luzardo C, Tápanes R, Pérez J

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Language: Spanish
References: 31
Page: 26-31
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ABSTRACT

Human T cell lymphotrophic virus I (HTLV-I) is a retrovirus thatcauses adult cell leukemia and the neurological disorder knownas tropical spastic paraparesis. Pathology apparently resultsfrom the expression of viral and cellular genes which mightlead to cell transformation, immortalization and disease. Theaim of the present study is to compare levels of lipidperoxidation, peroxidation potential and sulfhydryl contentmeasured as oxidative stress indicators in 9 asymptomatic HTLV-Iseropositive (HTLV-I+) and 10 healthy individuals (HTLV-I -).These parameters are related to changes in the levels of CD3, CD4,CD8 and CD25 T cell subsets. Spectrophotometric techniques wereassayed for evaluation of oxidative stress indicators and flowcytometry for determination of T cell subsets. Significantdifferences were found (p ‹ 0,05) between HTLV-I+ and HTLV-I-subjects regarding to stress indicators, except the concentrationof total sulfhydryl group. The CD4+ T lymphocytes count staysinside the normal intervals whereas the CD8+ T lymphocytescount was slightly increased. Relative values of CD25 alsoshowed significant differences between HTLV-I+ and HTLV-I-subjects. These results reveal both the presence of a pro-oxidantstate with the activation of the cytotoxic cellular response andthe activation of cellular gene expression. These determinationsmay be of interest for knowledge of physiopathology of theinfection and for possible preventive therapeutic interventions.

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