2020, Number 1
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AbanicoVet 2020; 10 (1)
Evaluation of oocyst shedding of Eimeria maxima and Eimeria acervulina in broiler
Laverty L, Señas-Cuesta R, Martínez-González S, Selby C, Tellez-Jr G, Hernandez-Velasco X, Vuong C, Hargis B, Tellez-Isaias G, Graham D
Language: Spanish
References: 22
Page: 1-11
PDF size: 554.69 Kb.
ABSTRACT
The purpose of the present study was to evaluate the day and the time of sample collection of an
experimental challenge with
Eimeria maxima (EM) and
Eimeria acervulina (EA) in broiler chickens. Oneday
old male Cobb-Vantress broiler chickens were randomly allocated to one of three groups with ten
replicates (n=8 chickens/replicate). Chickens were placed in battery cages with a controlled age-appropriate
environment: Group 1) Negative control (no challenge or treatment); 2) Challenge control (Eimeria challenge
only); 3) Challenge + Salinomycin. Challenged chickens were orally gavaged with the mixed culture of
EM/EA (10,000 sporulated EM containing 4% wild-type EA) at 14 days of age. Performance parameters
were recorded at days 7, 14, 20, and 23. Lesions scores were recorded post-mortem on days 20 and 23.
Oocyst per gram (OPG) was performed on days six, seven, and eight post-challenge, and samples were
collected at 9:00 AM and 6:00 PM on each day, respectively. Oocyst counts were significantly different (P
‹ 0.05) between morning and afternoon on day six post coccidia challenge. The results of this study show
that the day and the time at which samples are collected can have a significant effect on the reliability and
validity of data.
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