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2020, Number 1

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AbanicoVet 2020; 10 (1)

Exploratory study of the genotoxicity from recombinant bovine tuberculosis vaccines

Ramos-Ibarra M, Villa-Castellanos J, Barba-León J, Flores-Valdez M, Zavala-Aguirre L, Torres BO

Language: Spanish
References: 27
Page: 1-14
PDF size: 941.10 Kb.


ABSTRACT

BCG vaccine (Calmette-Guérin Bacillus) for bovine tuberculous control has a variable efficiency, been necessary to produce and evaluate new vaccines. Genotoxicity of two new recombinant antituberculous potential vaccines was evaluated in Holstein Freisan calves using micronucleated erythrocytes (EMN). Five treatments were analyzed: 1) Saline solution, 2) pVAX1 vector (CV), 3) Micobacterium bovis (Mv) type 1 vaccine (PE11 [VR1]), 4) type 2 (Mv) vaccine (PPE68 [VR2]) and 5) both VR1 & VR2 vaccines. Five blood samples were taken to each organism. First sample was taken before any treatment. Samples 2 to 4 were taken every 24 hours; the fifth sample was taken at day 90 postreatment. The samples were analyzed with microscope to obtain EMN/10,000 total erythrocytes. We observed a decrease at EMN, related to age increase of the organisms (Kruskal Wallis, 95%). A comparison with control group at the end of the treatments showed significant EMN reductions at VR2 and VR1&VR2 groups (P=0.02). It seems that the results show a cytoprotecting effect but also could be a masked myelosuppressive effect (cytotoxicity) because the EMN frequency decreases with myelosuppression. It is necessary to continue with studies with young organisms in order to confirm toxicity.


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