González GN, Horta RM, Hernández AB, Fonte GL, García MG

Language: Spanish
References: 18
Page: 74-77
PDF size: 338.30 Kb.

ABSTRACT

Objective: to determine, by Real Time PCR, the frequency of Chlamydia trachomatis and Mycoplasma infections in women treated for infertility at the “Hermanos Ameijeiras” Hospital since February 2017 until January 2019.
Methods: The presence of C. trachomatis and Mycoplasma spp. was determined in vaginal samples of 436 females treated for infertility in the Genetic Department of the “Hermanos Ameijeiras” Hospital since February 2016 until January 2019. The determinations were made using the Light Mix kit C. trachomatis and Light Mix kit Mycoplasma gene/hom and Ureaplasma from TIB MOLBIOL, respectively, in a Cobas z480 thermal cycler (Roche). Frequency and percentage analyzes were performed using the statistical program SPSSv-20.
Results: Of the total of women studied (n=436) only 18 (4.1%) were positive for C. Trachomatis. The detection of Mycoplasma spp. showed that 171 (39.2 %) were positive for several of their species. Seven samples (1.61 %) showed coinfections by both bacterial.
Conclusions: The frequency of infection by C. trachomatis in women studied for infertility is low; on the other hand, those found for Mycoplasma spp. are relatively high.

REFERENCES

1. Newman L, Rowley J, Vander Hoorn S, Wijesooriya NS, Unemo M, Low N, et al. Global Estimates of the Prevalence and Incidence of Four Curable Sexually Transmitted Infections in 2012 Based on Systematic Review and Global Reporting. PloS one. 2015;10(12):e0143304-e.

2. Elwell C., Mirrashidi K., & Engel J. (2016). Chlamydia cell biology and pathogenesis. Nature reviews. Microbiology, 14(6), 385–400. doi:10.1038/nrmicro.2016.30.

3. Rivero D, Kourí V, Correa C, Martínez I, Llanes RI, Barreal RT, et al. Detección de Chlamydia trachomatis en muestras de exudado endocervical mediante una prueba de diagnóstico rápido y dos técnicas de reacción en cadena de la polimerasa. Revista Cubana de Endocrinología y Obstetricia [Internet] 2014 [citado 12 jul 2019];40(1):48-57.Disponible en Available from: http://scielo.sld.cu/pdf/gin/v40n1/gin06114.pdf

4. Panzetta ME, Valdivia RH, Saka HA. Chlamydia Persistence: A Survival Strategy to Evade Antimicrobial Effects in-vitro and in-vivo. Front Microbiol. 2018;9:3101. Published 2018 Dec 12. doi:10.3389/fmicb.2018.03101

5. Akande V, Turner C, Horner P, Horne A, Pacey A, British Fertility S. Impact of Chlamydia trachomatis in the reproductive setting: British Fertility Society Guidelines for practice. Human fertility (Cambridge, England). 2010;13(3):115-25. ISSN1742-8149/1464-7273, doi: 10.3109/14647273.2010.513893

6. Taylor-Robinson D. Mollicutes in vaginal microbiology: Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum and Mycoplasma genitalium. Research in Microbiology. 2017; 168(9):875-81. https://doi.org/10.1016/j.resmic.2017.02.009.

7. Ljubin-Sternak S, Meštrović T. Chlamydia trachomatis and Genital Mycoplasmas: Pathogens with an Impact on Human Reproductive Health. J Pathog. 2014; 2014:183167. doi:10.1155/2014/183167.

8. Horner P, Donders G, Cusini M, Gomberg M, Jensen JS, Unemo M. Should we be testing for urogenital Mycoplasma hominis, Ureaplasma parvum and Ureaplasma urealyticum in men and women? – a position statement from the European STI Guidelines Editorial Board. Journal of the European Academy of Dermatology and Venereology. 2018;32(11):1845-51. doi:10.1111/jdv.15146.

9. Dhawan B, Rawre J, Ghosh A, Malhotra N, Ahmed MM, Sreenivas V, et al. Diagnostic efficacy of a real time-PCR assay for Chlamydia trachomatis infection in infertile women in north India. The Indian journal of medical research. 2014;140(2):252-61. PubMed PMID: 25297359; PubMed Central PMCID: PMC4216500.

10. Declaración de Helsinki de la AMM: Asociación Médica Mundial; 2013 Contract No: Document Number |.

11. Frontela Noda M, Rodríguez Marín Y, Verdejas Varela OL, Valdés Martínez FJ. Infección por Chlamydia trachomatis en mujeres cubanas en edad reproductiva. Revista Cubana de Endocrinología Rev Cubana Endocrinol [Internet] 2006 [citado 12 jul 2019];17(2):1-.Disponible en: http://scielo.sld.cu/pdf/end/v17n2/end01206.pdf

12. García-Arata MI, González C, Álvarez-Santas E, Galán JC, Jaqueti J, Molina L, et al. Diagnostic bacteriology – non-culture based, including molecular and MALDI-TOF. In: ESCMID. Tco, editor. Real-time PCR for detection of Chlamydia trachomatis and Neisseria gonorrhoeae from clinical samples 2017; EV015 Molecular diagnostics and MALDI-TOF. 2017.

13. Pimenta J. Screening for genital chlamydial infection. British Medical Journal. 2000;321:629-31.doi: https://doi.org/10.1136/ bmj.321.7261.629

14. Mertz JK. Trends in the prevalence of Chlamydia infections: impact of community-wide testing. Sexually transmitted diseases. 1997;24:169-75. PMID: 9132985 Issn Print: 0148-5717

15. Ghazal-Aswad S. Prevalence of Chlamydia trachomatis infection among women in a Middle Eastern community. BMC women’s health. 2004;4:3 doi:10.1186/1472-6874-4-3.

16. Witkin SS, Minis E, Athanasiou A, Leizer J, Linhares IM. Chlamydia trachomatis: the Persistent Pathogen. Clinical and vaccine immunology :CVI.2017;24(10):e00203-17.ISNN:1556-679X1556-6811doi: 10.1128/CVI.00203-17

17. Suchland RJ, Rockey DD, Putman TE, Dimond ZE. Demonstration of Persistent Infections and Genome Stability by Whole-Genome Sequencing of Repeat-Positive, Same-Serovar Chlamydia trachomatis Collected From the Female Genital Tract. The Journal of Infectious Diseases. 2017;215(11):1657-65.ISNN 0022-1899 doi: 10.1093/infdis/jix155

18. Rashidi BH, Chamani-Tabriz L, Haghollahi F, Jeddi-Tehrani M, Naghizadeh MM, Shariat M, et al. Effects of Chlamydia trachomatis Infection on Fertility; A Case-Control Study. Journal of Reproduction & Infertility. 2013 Apr-Jun;14(2):67-72.ISNN 2228-5482/2251-676X URL https://www.ncbi.nlm.nih.gov/pubmed/23926567